{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:38:41Z","timestamp":1760985521808,"version":"build-2065373602"},"reference-count":85,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2016,12,21]],"date-time":"2016-12-21T00:00:00Z","timestamp":1482278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteomes"],"abstract":"<jats:p>Proteomics is a field of growing importance in animal and aquatic sciences. Similar to other proteomic approaches, top-down proteomics is slowly making its way within the vast array of proteomic approaches that researchers have access to. This opinion and mini-review article is dedicated to top-down proteomics and how its use can be of importance to animal and aquatic sciences. Herein, we include an overview of the principles of top-down proteomics and how it differs regarding other more commonly used proteomic methods, especially bottom-up proteomics. In addition, we provide relevant sections on how the approach was or can be used as a research tool and conclude with our opinions of future use in animal and aquatic sciences.<\/jats:p>","DOI":"10.3390\/proteomes4040038","type":"journal-article","created":{"date-parts":[[2016,12,23]],"date-time":"2016-12-23T04:09:09Z","timestamp":1482466149000},"page":"38","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Top-Down Proteomics and Farm Animal and Aquatic Sciences"],"prefix":"10.3390","volume":"4","author":[{"given":"Alexandre","family":"Campos","sequence":"first","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, 4450-208 Matosinhos, Portugal"}]},{"given":"Andr\u00e9","family":"De Almeida","sequence":"additional","affiliation":[{"name":"Clinical Department, RUSVM\u2014Ross University School of Veterinary Medicine, PO Box 334, Basseterre, Saint Kitts and Nevis"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4187","DOI":"10.1016\/j.jprot.2012.05.014","article-title":"Proteomics, a new tool for farm animal science","volume":"75","author":"Eckersall","year":"2012","journal-title":"J. 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