{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:19:17Z","timestamp":1762867157117,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2017,6,25]],"date-time":"2017-06-25T00:00:00Z","timestamp":1498348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Digital Microfluidics (DMF) has emerged as a disruptive methodology for the control and manipulation of low volume droplets. In DMF, each droplet acts as a single reactor, which allows for extensive multiparallelization of biological and chemical reactions at a much smaller scale. DMF devices open entirely new and promising pathways for multiplex analysis and reaction occurring in a miniaturized format, thus allowing for healthcare decentralization from major laboratories to point-of-care with accurate, robust and inexpensive molecular diagnostics. Here, we shall focus on DMF platforms specifically designed for nucleic acid amplification, which is key for molecular diagnostics of several diseases and conditions, from pathogen identification to cancer mutations detection. Particular attention will be given to the device architecture, materials and nucleic acid amplification applications in validated settings.<\/jats:p>","DOI":"10.3390\/s17071495","type":"journal-article","created":{"date-parts":[[2017,6,27]],"date-time":"2017-06-27T02:58:05Z","timestamp":1498532285000},"page":"1495","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Digital Microfluidics for Nucleic Acid Amplification"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3359-3773","authenticated-orcid":false,"given":"Beatriz","family":"Coelho","sequence":"first","affiliation":[{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"given":"Bruno","family":"Veigas","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"},{"name":"UCIBIO, Departamento de Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"given":"Elvira","family":"Fortunato","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1997-7669","authenticated-orcid":false,"given":"Rodrigo","family":"Martins","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7350-649X","authenticated-orcid":false,"given":"Hugo","family":"\u00c1guas","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6608-3422","authenticated-orcid":false,"given":"Rui","family":"Igreja","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"given":"Pedro","family":"Baptista","sequence":"additional","affiliation":[{"name":"UCIBIO, Departamento de Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2017,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.cbpa.2010.06.187","article-title":"Let\u2019s get digital: Digitizing chemical biology with microfluidics","volume":"14","author":"Jebrail","year":"2010","journal-title":"Curr. 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