{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T14:24:21Z","timestamp":1777040661353,"version":"3.51.4"},"reference-count":49,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T00:00:00Z","timestamp":1584144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UID\/CTM\/50025\/2019; UID\/Multi\/04378\/2019; Inn-INDIGO\/0002\/2015; PTDC\/BTM-SAL\/31201\/2017; SFRH\/BPD\/124311\/2016"],"award-info":[{"award-number":["UID\/CTM\/50025\/2019; UID\/Multi\/04378\/2019; Inn-INDIGO\/0002\/2015; PTDC\/BTM-SAL\/31201\/2017; SFRH\/BPD\/124311\/2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Microfluidic (MF) advancements have been leveraged toward the development of state-of-the-art platforms for molecular diagnostics, where isothermal amplification schemes allow for further simplification of DNA detection and quantification protocols. The MF integration with loop-mediated isothermal amplification (LAMP) is today the focus of a new generation of chip-based devices for molecular detection, aiming at fast and automated nucleic acid analysis. Here, we combined MF with droplet digital LAMP (ddLAMP) on an all-in-one device that allows for droplet generation, target amplification, and absolute quantification. This multilayer 3D chip was developed in less than 30 minutes by using a low-cost and extremely adaptable production process that exploits direct laser writing technology in \u201cShrinky-dinks\u201d polystyrene sheets. ddLAMP and target quantification were performed directly on-chip, showing a high correlation between target concentration and positive droplet score. We validated this integrated chip via the amplification of targets ranging from five to 500,000 copies\/reaction. Furthermore, on-chip amplification was performed in a 10 \u00b5L volume, attaining a limit of detection of five copies\/\u00b5L under 60 min. This technology was applied to quantify a cancer biomarker, c-MYC, but it can be further extended to any other disease biomarker.<\/jats:p>","DOI":"10.3390\/s20061624","type":"journal-article","created":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T08:20:44Z","timestamp":1584519644000},"page":"1624","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Fast Prototyping Microfluidics: Integrating Droplet Digital Lamp for Absolute Quantification of Cancer Biomarkers"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9935-4703","authenticated-orcid":false,"given":"Beatriz","family":"Oliveira","sequence":"first","affiliation":[{"name":"UCIBIO, Departamento de Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9265-4591","authenticated-orcid":false,"given":"Bruno","family":"Veigas","sequence":"additional","affiliation":[{"name":"UCIBIO, Departamento de Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"},{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2054-4438","authenticated-orcid":false,"given":"Alexandra R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"UCIBIO, Departamento de Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7350-649X","authenticated-orcid":false,"given":"Hugo","family":"\u00c1guas","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1997-7669","authenticated-orcid":false,"given":"Rodrigo","family":"Martins","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"given":"Elvira","family":"Fortunato","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5255-7095","authenticated-orcid":false,"given":"Pedro Viana","family":"Baptista","sequence":"additional","affiliation":[{"name":"UCIBIO, Departamento de Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Francesko, A., Cardoso, V.F., and Lanceros-M\u00e9ndez, S. 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