{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T14:56:05Z","timestamp":1775832965599,"version":"3.50.1"},"reference-count":21,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T00:00:00Z","timestamp":1669680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["CEECIND\/03986\/2018"],"award-info":[{"award-number":["CEECIND\/03986\/2018"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UIDB\/00127\/2020"],"award-info":[{"award-number":["UIDB\/00127\/2020"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["COMPETE2020"],"award-info":[{"award-number":["COMPETE2020"]}]},{"name":"IEETA\/UA R&amp;D unit","award":["CEECIND\/03986\/2018"],"award-info":[{"award-number":["CEECIND\/03986\/2018"]}]},{"name":"IEETA\/UA R&amp;D unit","award":["UIDB\/00127\/2020"],"award-info":[{"award-number":["UIDB\/00127\/2020"]}]},{"name":"IEETA\/UA R&amp;D unit","award":["COMPETE2020"],"award-info":[{"award-number":["COMPETE2020"]}]},{"name":"European Regional Development Fund","award":["CEECIND\/03986\/2018"],"award-info":[{"award-number":["CEECIND\/03986\/2018"]}]},{"name":"European Regional Development Fund","award":["UIDB\/00127\/2020"],"award-info":[{"award-number":["UIDB\/00127\/2020"]}]},{"name":"European Regional Development Fund","award":["COMPETE2020"],"award-info":[{"award-number":["COMPETE2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Pain is a complex phenomenon that arises from the interaction of multiple neuroanatomic and neurochemical systems with several cognitive and affective processes. Nowadays, the assessment of pain intensity still relies on the use of self-reports. However, recent research has shown a connection between the perception of pain and exacerbated stress response in the Autonomic Nervous System. As a result, there has been an increasing analysis of the use of autonomic reactivity with the objective to assess pain. In the present study, the methods include pre-processing, feature extraction, and feature analysis. For the purpose of understanding and characterizing physiological responses of pain, different physiological signals were, simultaneously, recorded while a pain-inducing protocol was performed. The obtained results, for the electrocardiogram (ECG), showed a statistically significant increase in the heart rate, during the painful period compared to non-painful periods. Additionally, heart rate variability features demonstrated a decrease in the Parasympathetic Nervous System influence. The features from the electromyogram (EMG) showed an increase in power and contraction force of the muscle during the pain induction task. Lastly, the electrodermal activity (EDA) showed an adjustment of the sudomotor activity, implying an increase in the Sympathetic Nervous System activity during the experience of pain.<\/jats:p>","DOI":"10.3390\/s22239276","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T02:09:58Z","timestamp":1669687798000},"page":"9276","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of Physiological Responses during Pain Induction"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5717-1415","authenticated-orcid":false,"given":"Raquel","family":"Sebasti\u00e3o","sequence":"first","affiliation":[{"name":"IEETA, DETI, LASI, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Ana","family":"Bento","sequence":"additional","affiliation":[{"name":"DFis, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8650-9219","authenticated-orcid":false,"given":"Susana","family":"Br\u00e1s","sequence":"additional","affiliation":[{"name":"IEETA, DETI, LASI, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1976","DOI":"10.1097\/j.pain.0000000000001939","article-title":"The revised International Association for the Study of Pain definition of pain: Concepts, challenges, and compromises","volume":"161","author":"Raja","year":"2020","journal-title":"Pain"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1016\/j.pain.2012.05.010","article-title":"Multi-parameter autonomic-based pain assessment: More is more?","volume":"153","author":"Loggia","year":"2012","journal-title":"Pain"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1111\/anae.13018","article-title":"Assessing pain objectively: The use of physiological markers","volume":"70","author":"Cowen","year":"2015","journal-title":"Anaesthesia"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/0031-9384(90)90064-B","article-title":"Influence of cold pain in the hand on skin impedance, heart rate and skin temperature","volume":"47","author":"Hampf","year":"1990","journal-title":"Physiol. 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