{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T04:16:49Z","timestamp":1776485809402,"version":"3.51.2"},"reference-count":24,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:00:00Z","timestamp":1672963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT\u2014Foundation for Science and Technology","doi-asserted-by":"publisher","award":["BD 138724\/2018"],"award-info":[{"award-number":["BD 138724\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT\u2014Foundation for Science and Technology","doi-asserted-by":"publisher","award":["UIDB\/05183\/2020"],"award-info":[{"award-number":["UIDB\/05183\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT\u2014Foundation for Science and Technology","doi-asserted-by":"publisher","award":["UID\/05367\/2020"],"award-info":[{"award-number":["UID\/05367\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MED\u2013Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, \u00c9vora University","award":["BD 138724\/2018"],"award-info":[{"award-number":["BD 138724\/2018"]}]},{"name":"MED\u2013Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, \u00c9vora University","award":["UIDB\/05183\/2020"],"award-info":[{"award-number":["UIDB\/05183\/2020"]}]},{"name":"MED\u2013Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, \u00c9vora University","award":["UID\/05367\/2020"],"award-info":[{"award-number":["UID\/05367\/2020"]}]},{"name":"PROGRAMATICO\u2014Research Unit INESC MN, Microsystems and Nanotechnologies)","award":["BD 138724\/2018"],"award-info":[{"award-number":["BD 138724\/2018"]}]},{"name":"PROGRAMATICO\u2014Research Unit INESC MN, Microsystems and Nanotechnologies)","award":["UIDB\/05183\/2020"],"award-info":[{"award-number":["UIDB\/05183\/2020"]}]},{"name":"PROGRAMATICO\u2014Research Unit INESC MN, Microsystems and Nanotechnologies)","award":["UID\/05367\/2020"],"award-info":[{"award-number":["UID\/05367\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The potato cyst nematode (PCN), Globodera pallida, has acquired significant importance throughout Europe due to its widespread prevalence and negative effects on potato production. Thus, rapid and reliable diagnosis of PCN is critical during surveillance programs and for the implementation of control measures. The development of innovative technologies to overcome the limitations of current methodologies in achieving early detection is needed. Lab-on-a-chip devices can swiftly and accurately detect the presence of certain nucleotide sequences with high sensitivity and convert the presence of biological components into an understandable electrical signal by combining biosensors with microfluidics-based biochemical analysis. In this study, a specific DNA-probe sequence and PCR primers were designed to be used in a magnetoresistive biosensing platform to amplify the internal transcribed spacer region of the ribosomal DNA of G. pallida. Magnetic nanoparticles were used as the labelling agents of asymmetric PCR product through biotin\u2013streptavidin interaction. Upon target hybridization to sensor immobilized oligo probes, the fringe field created by the magnetic nanoparticles produces a variation in the sensor\u2019s electrical resistance. The detection signal corresponds to the concentration of target molecules present in the sample. The results demonstrate the suitability of the magnetic biosensor to detect PCR target product and the specificity of the probe, which consistently distinguishes G. pallida (DV\/V &gt; 1%) from other cyst nematodes (DV\/V &lt; 1%), even when DNA mixtures were tested at different concentrations. This shows the magnetic biosensor\u2019s potential as a bioanalytical device for field applications and border phytosanitary inspections.<\/jats:p>","DOI":"10.3390\/s23020647","type":"journal-article","created":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T03:19:54Z","timestamp":1672975194000},"page":"647","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2432-1234","authenticated-orcid":false,"given":"Maria Jo\u00e3o","family":"Camacho","sequence":"first","affiliation":[{"name":"INIAV, I.P.\u2014National Institute for Agriculture and Veterinary Research, 2780-159 Oeiras, Portugal"},{"name":"NemaLab, MED\u2013Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, University of \u00c9vora, 7006-554 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3076-6686","authenticated-orcid":false,"given":"D\u00e9bora C.","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"INESC-MN- Microsystems and Nanotechnologies, 1000-029 Lisbon, Portugal"},{"name":"IST\u2014Instituto Superior T\u00e9cnico, University of Lisbon, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6781-5234","authenticated-orcid":false,"given":"Eug\u00e9nia","family":"de Andrade","sequence":"additional","affiliation":[{"name":"INIAV, I.P.\u2014National Institute for Agriculture and Veterinary Research, 2780-159 Oeiras, Portugal"},{"name":"GREEN-IT Bioresources for Sustainability, ITQB NOVA, 2780-157 Oeiras, Portugal"}]},{"given":"Ver\u00f3nica C.","family":"Martins","sequence":"additional","affiliation":[{"name":"INESC-MN- Microsystems and Nanotechnologies, 1000-029 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6883-5288","authenticated-orcid":false,"given":"Maria L.","family":"In\u00e1cio","sequence":"additional","affiliation":[{"name":"INIAV, I.P.\u2014National Institute for Agriculture and Veterinary Research, 2780-159 Oeiras, Portugal"},{"name":"GREEN-IT Bioresources for Sustainability, ITQB NOVA, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4145-5031","authenticated-orcid":false,"given":"Manuel","family":"Mota","sequence":"additional","affiliation":[{"name":"NemaLab, MED\u2013Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, University of \u00c9vora, 7006-554 \u00c9vora, Portugal"}]},{"given":"Paulo P.","family":"Freitas","sequence":"additional","affiliation":[{"name":"INESC-MN- Microsystems and Nanotechnologies, 1000-029 Lisbon, Portugal"},{"name":"INL\u2014International Iberian Nanotechnology Laboratory, 4715-330 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,6]]},"reference":[{"key":"ref_1","unstructured":"Wollenweber, H.W. 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