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These applications, namely studies involving PPG data acquisition during day-to-day activities, require reliable and continuous measurements, which are often performed at the index finger or wrist. However, some PPG sensors are susceptible to saturation, motion artifacts, and discomfort upon their use. In this paper, an off-the-shelf PPG sensor was benchmarked and modified to improve signal saturation. Moreover, this paper explores the feasibility of using an optimized sensor in the lower limb as an alternative measurement site. Data were collected from 28 subjects with ages ranging from 18 to 59 years. To validate the sensors\u2019 performance, signal saturation and quality, wave morphology, performance of automatic systolic peak detection, and heart-rate estimation, were compared. For the upper and lower limb locations, the index finger and the first toe were used as reference locations, respectively. Lowering the amplification stage of the PPG sensor resulted in a significant reduction in signal saturation, from 18% to 0.5%. Systolic peak detection at rest using an automatic algorithm showed a sensitivity and precision of 0.99 each. The posterior wrist and upper arm showed pulse wave morphology correlations of 0.93 and 0.92, respectively. For these locations, peak detection sensitivity and precision were 0.95, 0.94 and 0.89, 0.89, respectively. Overall, the adjusted PPG sensors are a good alternative for obtaining high-quality signals at the fingertips, and for new measurement sites, the posterior pulse and the upper arm allow for high-quality signal extraction.<\/jats:p>","DOI":"10.3390\/s24010214","type":"journal-article","created":{"date-parts":[[2023,12,31]],"date-time":"2023-12-31T04:51:51Z","timestamp":1703998311000},"page":"214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Benchmarking of Sensor Configurations and Measurement Sites for Out-of-the-Lab Photoplethysmography"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3240-1164","authenticated-orcid":false,"given":"Max Nobre","family":"Supelnic","sequence":"first","affiliation":[{"name":"Department of Bioengineering (DBE), Instituto Superior T\u00e9cnico (IST), 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3604-1272","authenticated-orcid":false,"given":"Afonso Fortes","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2014Microsistemas e Nanotecnologias (INESC MN), 1000-029 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0514-7517","authenticated-orcid":false,"given":"Patr\u00edcia Justo","family":"Bota","sequence":"additional","affiliation":[{"name":"Department of Bioengineering (DBE), Instituto Superior T\u00e9cnico (IST), 1049-001 Lisbon, Portugal"},{"name":"Instituto de Telecomunica\u00e7\u00f5es (IT), 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0014-1543","authenticated-orcid":false,"given":"Lu\u00eds","family":"Br\u00e1s-Ros\u00e1rio","sequence":"additional","affiliation":[{"name":"Cardiology Department, Santa Maria University Hospital (CHLN), Lisbon Academic Medical Centre, 1649-028 Lisbon, Portugal"},{"name":"Cardiovascular Centre of the University of Lisbon, Lisbon School of Medicine, 1649-028 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6764-8432","authenticated-orcid":false,"given":"Hugo","family":"Pl\u00e1cido da Silva","sequence":"additional","affiliation":[{"name":"Department of Bioengineering (DBE), Instituto Superior T\u00e9cnico (IST), 1049-001 Lisbon, Portugal"},{"name":"Instituto de Telecomunica\u00e7\u00f5es (IT), 1049-001 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,29]]},"reference":[{"key":"ref_1","unstructured":"(2023, November 17). 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