{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T15:39:20Z","timestamp":1765381160112,"version":"3.46.0"},"reference-count":48,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["2022.07897.PTDC","2025.05501.BD","SFRH\/BPD\/116067\/2016"],"award-info":[{"award-number":["2022.07897.PTDC","2025.05501.BD","SFRH\/BPD\/116067\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union through Norte 2020\u2014European Regional Development Fund"},{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"crossref"}]},{"name":"PPBI\u2014Portuguese Platform of Bioimaging","award":["PPBI-POCI-01-0145-FEDER-022122","02\/C06-i06\/2024"],"award-info":[{"award-number":["PPBI-POCI-01-0145-FEDER-022122","02\/C06-i06\/2024"]}]},{"name":"FCT\/MCTES through national funds","award":["UID\/50006\/2025","UIDB\/04730\/2020","UIDP\/04730\/2020"],"award-info":[{"award-number":["UID\/50006\/2025","UIDB\/04730\/2020","UIDP\/04730\/2020"]}]},{"name":"FCT"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Dual-emission molecularly imprinted membranes (dual@MIPs@mbr) were developed as a proof-of-concept platform for the selective and instrument-free detection of the cancer biomarker carbohydrate antigen 19-9 (CA 19-9). The system integrates a ratiometric fluorescence response by embedding yellow-emitting quantum dots (y-QDs), serving as target-responsive probes, and blue-emitting carbon dots (b-CDs), acting as an internal reference, within a CA 19-9-imprinted polymeric matrix. Specific rebinding of CA 19-9 to the imprinted cavities induced selective quenching of the y-QDs while preserving the b-CDs emission, yielding a visible color shift from yellow\/green to blue. This behavior enabled the quantification of CA 19-9 over a linear range of 4\u2013400 U mL\u22121, with a limit of detection of 0.056 U mL\u22121 in diluted serum. The membranes showed good selectivity against common serum interferents and maintained short-term photochemical stability. Although the method has not yet been validated using real clinical samples, the pronounced ratiometric response and simple visual readout demonstrate its potential as a low-cost, portable sensing approach for future point-of-care cancer biomarker analysis.<\/jats:p>","DOI":"10.3390\/s25237363","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T18:42:02Z","timestamp":1764960122000},"page":"7363","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecularly Imprinted Membranes: Dual@MIPs@mbr for On-Site Detection of CA 19-9"],"prefix":"10.3390","volume":"25","author":[{"given":"Eduarda","family":"Rodrigues","sequence":"first","affiliation":[{"name":"CIETI-LabRISE, ISEP, Polytechnic of Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"}]},{"given":"Ana","family":"Xu","sequence":"additional","affiliation":[{"name":"CIETI-LabRISE, ISEP, Polytechnic of Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1148-2159","authenticated-orcid":false,"given":"Paula","family":"Sampaio","sequence":"additional","affiliation":[{"name":"i3S\u2014Institute of Research and Innovation in Health, University of Porto, 4200-135 Porto, Portugal"},{"name":"IBMC\u2014Institute for Molecular and Cell Biology, University of Porto, 4150-180 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8722-3172","authenticated-orcid":false,"given":"Rafael","family":"Castro","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira No 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7879-0143","authenticated-orcid":false,"given":"David","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira No 228, 4050-313 Porto, Portugal"}]},{"given":"Jo\u00e3o","family":"Santos","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira No 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2374-2467","authenticated-orcid":false,"given":"Ana","family":"Piloto","sequence":"additional","affiliation":[{"name":"CIETI-LabRISE, ISEP, Polytechnic of Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"},{"name":"LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira No 228, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1533033820962117","DOI":"10.1177\/1533033820962117","article-title":"Pancreatic Cancer: A Review of Risk Factors, Diagnosis, and Treatment","volume":"19","author":"Zhao","year":"2020","journal-title":"Technol. 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