{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T03:34:00Z","timestamp":1764646440981,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T00:00:00Z","timestamp":1679011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["2020.06656.BD"],"award-info":[{"award-number":["2020.06656.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Separations"],"abstract":"<jats:p>Moringa oleifera L. tree (Mo) has emerged as a rich alternative source of bioactive compounds to design cosmetic formulations. Supercritical carbon dioxide fluid extraction (SFE-CO2) was successfully applied on the screening of Mo seed, leaf, and root extracts. The extraction yield was evaluated by response surface methodology (RSM), for pressure and temperature ranges of 117\u2013273 bar and 41\u201360 \u00b0C, respectively, using a design of experiments (DOE). The pressure significantly affected the results (\u03b1=0.05), with the highest extraction efficiency obtained at conditions above 195 bar. The extracts\u2019 composition, evaluated by gas chromatography-mass spectrometer (GC-MS), revealed an increasing correlation between the pressure, total extract solubility, and mass of extract at a constant temperature, due to the higher extraction yield. Seed extracts presented more than 80% of oleic acid in relative composition (8.04 mgcompound\u2219gplantpart\u22121). Leaf extracts performed well for the obtainment of linolenic acid (&gt;20%; 3.10 mg\u2219g\u22121), nonacosane (&gt;22%; 0.46 mg\u2219g\u22121), and \u03b1-tocopherol (&gt;20%; 0.21 mg\u2219g\u22121). Mo root resulted in higher relative composition for sterol molecules, despite its very low affinity with CO2. The most promising bioactive compounds, oleic acid and \u03b1-tocopherol, were more abundant when operating at 250 bar at 45 \u00b0C and 195 bar at 55 \u00b0C, for Mo seed and leaf SFE-CO2 extracts, respectively.<\/jats:p>","DOI":"10.3390\/separations10030210","type":"journal-article","created":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T03:09:37Z","timestamp":1679281777000},"page":"210","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Moringa oleifera L. Screening: SFE-CO2 Optimisation and Chemical Composition of Seed, Leaf, and Root Extracts as Potential Cosmetic Ingredients"],"prefix":"10.3390","volume":"10","author":[{"given":"J\u00falia C.","family":"Kessler","sequence":"first","affiliation":[{"name":"LSRE-LCM\u2014Laboratory of Separation and Reaction Engineering\u2013Laboratory of Catalysis and Materials, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Laborat\u00f3rio Para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"given":"Yaidelin A.","family":"Manrique","sequence":"additional","affiliation":[{"name":"LSRE-LCM\u2014Laboratory of Separation and Reaction Engineering\u2013Laboratory of Catalysis and Materials, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Isabel M.","family":"Martins","sequence":"additional","affiliation":[{"name":"LSRE-LCM\u2014Laboratory of Separation and Reaction Engineering\u2013Laboratory of Catalysis and Materials, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0715-4761","authenticated-orcid":false,"given":"Al\u00edrio E.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"LSRE-LCM\u2014Laboratory of Separation and Reaction Engineering\u2013Laboratory of Catalysis and Materials, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6844-333X","authenticated-orcid":false,"given":"Maria Filomena","family":"Barreiro","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Laborat\u00f3rio Para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"given":"Madalena M.","family":"Dias","sequence":"additional","affiliation":[{"name":"LSRE-LCM\u2014Laboratory of Separation and Reaction Engineering\u2013Laboratory of Catalysis and Materials, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108","DOI":"10.3389\/fphar.2018.00108","article-title":"Moringa genus: A review of phytochemistry and pharmacology","volume":"9","author":"Rani","year":"2018","journal-title":"Front. 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