{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:00:13Z","timestamp":1770980413496,"version":"3.50.1"},"reference-count":28,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Fundac\u0327\u00e3o para a Ci\u00eancia e Tecnologia and Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior","doi-asserted-by":"publisher","award":["UIDB\/50006\/2020|UIDP\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020|UIDP\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Fundac\u0327\u00e3o para a Ci\u00eancia e Tecnologia and Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior","doi-asserted-by":"publisher","award":["SFRH\/BPD\/111181\/2015"],"award-info":[{"award-number":["SFRH\/BPD\/111181\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT","doi-asserted-by":"publisher","award":["UIDB\/50006\/2020|UIDP\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020|UIDP\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT","doi-asserted-by":"publisher","award":["SFRH\/BPD\/111181\/2015"],"award-info":[{"award-number":["SFRH\/BPD\/111181\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Separations"],"abstract":"<jats:p>This study aimed to investigate the influence of various production stages on the quality and spoilage conditions of traditionally dry-cured chorizo. To accomplish this, we employed an experimental design that examined three key production parameters: the batch, the filling stage, and the food smoking process. The study was conducted in collaboration with a local producer who adheres to traditional curing methods utilizing oak wood smoke and heat. Biogenic amine levels were closely monitored throughout the process. This involved their extraction and derivatization through the salting-out technique, followed by identification and quantification using LC-ESI\/MSn and HPLC-DAD, respectively. The findings suggest that both raw materials and the production process are well controlled during the filling stage. However, it became evident that the 14-day oak wood smoking period had a significant impact on biogenic amine formation, whose total mean values increased from 126 to 1385 mg kg\u22121, particularly with respect to putrescine (PUT), cadaverine (CAD), and tyramine (TYR), although these levels remained below the oral toxicity limit (2000 mg kg\u22121). Consequently, the concentration of these compounds can influence the quality and safety of traditionally dry-cured chorizos. Therefore, the combined levels of PUT, CAD, and TYR can serve as a valuable quality indicator for these products.<\/jats:p>","DOI":"10.3390\/separations10120585","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T03:35:06Z","timestamp":1701056106000},"page":"585","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of the Smoking Process on Biogenic Amine Levels in Traditional Dry-Cured Chorizo"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1438-1353","authenticated-orcid":false,"given":"In\u00eas M.","family":"Valente","sequence":"first","affiliation":[{"name":"LAQV\/REQUIMTE, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"given":"Cl\u00e1udia S.","family":"Sousa","sequence":"additional","affiliation":[{"name":"LAQV\/REQUIMTE, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9441-7867","authenticated-orcid":false,"given":"Lu\u00eds F.","family":"Guido","sequence":"additional","affiliation":[{"name":"LAQV\/REQUIMTE, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.meatsci.2007.08.009","article-title":"A comparison of the physicochemical and sensory characteristics of alheira samples from different-sized producers","volume":"79","author":"Patarata","year":"2008","journal-title":"Meat Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1111\/j.1365-2672.2008.03947.x","article-title":"Characterization of microbial population of \u2018Alheira\u2019 (a traditional Portuguese fermented sausage) by PCR-DGGE and traditional cultural microbiological methods","volume":"105","author":"Albano","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1016\/j.fct.2012.03.054","article-title":"Comparative survey of PAHs incidence in Portuguese traditional meat and blood sausages","volume":"50","author":"Roseiro","year":"2012","journal-title":"Food Chem. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.meatsci.2012.09.016","article-title":"Nutritional value and influence of the thermal processing on a traditional Portuguese fermented sausage (alheira)","volume":"93","author":"Campos","year":"2013","journal-title":"Meat Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.meatsci.2006.02.011","article-title":"Chemical and microbiological characterization of alheira: A typical Portuguese fermented sausage with particular reference to factors relating to food safety","volume":"73","author":"Ferreira","year":"2006","journal-title":"Meat Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.fm.2006.12.007","article-title":"Chemical and microbiological characterisation of \u201cSalpicao de Vinhais\u201d and \u201cChourica de Vinhais\u201d: Traditional dry sausages produced in the North of Portugal","volume":"24","author":"Ferreira","year":"2007","journal-title":"Food Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.1016\/j.fct.2011.06.036","article-title":"Polycyclic aromatic hydrocarbons incidence in Portuguese traditional smoked meat products","volume":"49","author":"Santos","year":"2011","journal-title":"Food Chem. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7925","DOI":"10.3390\/nu7095371","article-title":"Food Processing and the Mediterranean Diet","volume":"7","author":"Hoffman","year":"2015","journal-title":"Nutrients"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0378-4347(01)00438-8","article-title":"Determination of polycyclic aromatic hydrocarbons in smoked meat products and smoke flavouring food additives","volume":"770","author":"Simko","year":"2002","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.meatsci.2012.06.004","article-title":"Profiles and concentrations of heterocyclic aromatic amines formed in beef during various heat treatments depend on the time of ripening and muscle type","volume":"92","author":"Szterk","year":"2012","journal-title":"Meat Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6903","DOI":"10.1002\/fsn3.2633","article-title":"Chemical hazards in smoked meat and fish","volume":"9","author":"Kpoclou","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Serio, A., Laika, J., Maggio, F., Sacchetti, G., D\u2019Alessandro, F., Rossi, C., Martuscelli, M., Chaves-Lopez, C., and Paparella, A. (2020). Casing Contribution to Proteolytic Changes and Biogenic Amines Content in the Production of an Artisanal Naturally Fermented Dry Sausage. Foods, 9.","DOI":"10.3390\/foods9091286"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0168-1605(03)00080-1","article-title":"Biogenic amines in dry fermented sausages: A review","volume":"88","author":"Suzzi","year":"2003","journal-title":"Int. J. Food Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1590\/S1517-83822013000200010","article-title":"The influences of fish infusion broth on the biogenic amines formation by lactic acid bacteria","volume":"44","author":"Kuley","year":"2013","journal-title":"Braz. J. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2634","DOI":"10.1002\/jsfa.10928","article-title":"Biogenic amines: Formation, action and toxicity\u2014A review","volume":"101","author":"Lukasiewicz","year":"2021","journal-title":"J. Sci. Food Agric."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Gardini, F., Ozogul, Y., Suzzi, G., Tabanelli, G., and Ozogul, F. (2016). Technological Factors Affecting Biogenic Amine Content in Foods: A Review. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.01218"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.talanta.2014.02.026","article-title":"Analysis of biogenic amines in wines by salting-out assisted liquid-liquid extraction and high-performance liquid chromatography with fluorimetric detection","volume":"124","author":"Ramos","year":"2014","journal-title":"Talanta"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.chroma.2013.08.014","article-title":"Another glimpse over the salting-out assisted liquid-liquid extraction in acetonitrile\/water mixtures","volume":"1308","author":"Valente","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2392","DOI":"10.1007\/s12161-019-01587-4","article-title":"Development of an Analytical Method for the Simultaneous Measurement of 10 Biogenic Amines in Meat: Application to Beninese Grilled Pork Samples","volume":"12","author":"Douny","year":"2019","journal-title":"Food Anal. Method"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"112684","DOI":"10.1016\/j.foodres.2023.112684","article-title":"Novel insights from protein degradation: Deciphering the dynamic evolution of biogenic amines as a quality indicator in pork during storage","volume":"167","author":"Gu","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Visciano, P., Schirone, M., and Paparella, A. (2020). An Overview of Histamine and Other Biogenic Amines in Fish and Fish Products. Foods, 9.","DOI":"10.3390\/foods9121795"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/j.foodchem.2009.03.061","article-title":"Effect of intensity of smoking treatment on the free amino acids and biogenic amines occurrence in dry cured ham","volume":"116","author":"Martuscelli","year":"2009","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1778","DOI":"10.1016\/j.foodchem.2011.05.106","article-title":"Reduced biogenic amine contents in via addition of selected lactic acid bacteria","volume":"129","author":"Rabie","year":"2011","journal-title":"Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"107115","DOI":"10.1016\/j.foodcont.2020.107115","article-title":"Contamination of traditionally smoked cheeses with polycyclic aromatic hydrocarbons and biogenic amines","volume":"112","author":"Domagala","year":"2020","journal-title":"Food Control"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1007\/s10068-017-0239-3","article-title":"Biogenic amines in foods","volume":"26","author":"Doeun","year":"2017","journal-title":"Food Sci. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1007\/s13197-016-2224-x","article-title":"Biogenic amines in seafood: A review","volume":"53","author":"Biji","year":"2016","journal-title":"J. Food Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"127303","DOI":"10.1016\/j.foodchem.2020.127303","article-title":"The biogenic amine tryptamine, unlike \u03b2-phenylethylamine, shows cytotoxicity at concentrations that have been found in foods","volume":"331","author":"Redruello","year":"2020","journal-title":"Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1080\/19393210.2023.2186489","article-title":"Biogenic amines and volatile -nitrosamines in Chinese smoked-cured bacon (Larou) from industrial and artisanal origins","volume":"16","author":"Li","year":"2023","journal-title":"Food Addit. Contam. Part B-Surveill."}],"container-title":["Separations"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2297-8739\/10\/12\/585\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:31:12Z","timestamp":1760131872000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2297-8739\/10\/12\/585"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,27]]},"references-count":28,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["separations10120585"],"URL":"https:\/\/doi.org\/10.3390\/separations10120585","relation":{},"ISSN":["2297-8739"],"issn-type":[{"value":"2297-8739","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,27]]}}}