{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T22:46:37Z","timestamp":1772837197193,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dire\u00e7\u00e3o Regional da Ci\u00eancia e Tecnologia (DRCT) funds","award":["M1.1.C\/PROJ.EXPLORAT\u00d3RIOS\/003\/2022\u2014PotBioCJap"],"award-info":[{"award-number":["M1.1.C\/PROJ.EXPLORAT\u00d3RIOS\/003\/2022\u2014PotBioCJap"]}]},{"name":"Dire\u00e7\u00e3o Regional da Ci\u00eancia e Tecnologia (DRCT) funds","award":["M3.1.a\/F\/008\/2021"],"award-info":[{"award-number":["M3.1.a\/F\/008\/2021"]}]},{"name":"Fundo Regional da Ci\u00eancia e Tecnologia (FRCT)","award":["M1.1.C\/PROJ.EXPLORAT\u00d3RIOS\/003\/2022\u2014PotBioCJap"],"award-info":[{"award-number":["M1.1.C\/PROJ.EXPLORAT\u00d3RIOS\/003\/2022\u2014PotBioCJap"]}]},{"name":"Fundo Regional da Ci\u00eancia e Tecnologia (FRCT)","award":["M3.1.a\/F\/008\/2021"],"award-info":[{"award-number":["M3.1.a\/F\/008\/2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Separations"],"abstract":"<jats:p>In the Azores Archipelago, Cryptomeria japonica is, currently, the most cultivated forestry tree for timber production, landscaping, and gardening, generating large amounts of foliage waste that is used for local essential oils (EOs) production. However, the existing literature on the biological potential of EOs from different C. japonica foliage parts, such as female cones (FC), remains limited. Thus, in the present study, EOs extracted by hydrodistillation from Azorean C. japonica immature and mature FC (IFC and MFC), as well as some major EO components, were screened for their: (i) antioxidant capacity, evaluated by DPPH free-radical-scavenging activity (FRSA) and \u03b2-carotene-linoleic acid bleaching activity (BCBA), (ii) antimicrobial activities, assessed by the disc diffusion method against eight bacteria and one fungus, and (iii) toxicity against Artemia salina. Among both FC EO samples, the IFC EO exhibited the best DPPH\u2013FRSA, BCBA, and growth inhibitory activity against Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, and Penicillium italicum, as well as a slightly increased toxic potential, due to their differential compositions, as assessed by GC\u2013MS analysis. Thus, the FC\u2019s maturation process decreased their EOs\u2019 bioactivities. In conclusion, this finding could help in determining the optimal developmental stage for enhancing the antioxidant and antimicrobial compounds content in FC EOs. In turn, this contributes to increasing the commercial potential of C. japonica\u2019s EO industry.<\/jats:p>","DOI":"10.3390\/separations11040102","type":"journal-article","created":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T11:11:09Z","timestamp":1712229069000},"page":"102","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Variations in Essential Oil Biological Activities of Female Cones at Different Developmental Stages from Azorean Cryptomeria japonica (Thunb. ex L.f.) D. Don (Cupressaceae)"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6897-4886","authenticated-orcid":false,"given":"Alexandre","family":"Janeiro","sequence":"first","affiliation":[{"name":"Institute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"},{"name":"Department of Physics, Chemistry and Engineering (DCFQE), Faculty of Science and Technology, University of the Azores, 9500-321 Ponta Delgada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5139-0828","authenticated-orcid":false,"given":"Ana","family":"Lima","sequence":"additional","affiliation":[{"name":"Institute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"},{"name":"Department of Physics, Chemistry and Engineering (DCFQE), Faculty of Science and Technology, University of the Azores, 9500-321 Ponta Delgada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3527-6237","authenticated-orcid":false,"given":"Filipe","family":"Arruda","sequence":"additional","affiliation":[{"name":"Institute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"},{"name":"Department of Biology (DB), Faculty of Science and Technology, University of the Azores, 9500-321 Ponta Delgada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0200-9768","authenticated-orcid":false,"given":"Tanner","family":"Wortham","sequence":"additional","affiliation":[{"name":"The Perfumery, 621 Park East Blvd, New Albany, IN 47150, USA"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8718-7575","authenticated-orcid":false,"given":"T\u00e2nia","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Biology (DB), Faculty of Science and Technology, University of the Azores, 9500-321 Ponta Delgada, Portugal"}]},{"given":"Jos\u00e9","family":"Baptista","sequence":"additional","affiliation":[{"name":"Institute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"},{"name":"Department of Physics, Chemistry and Engineering (DCFQE), Faculty of Science and Technology, University of the Azores, 9500-321 Ponta Delgada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6736-8729","authenticated-orcid":false,"given":"Elisabete","family":"Lima","sequence":"additional","affiliation":[{"name":"Institute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"},{"name":"Department of Physics, Chemistry and Engineering (DCFQE), Faculty of Science and Technology, University of the Azores, 9500-321 Ponta Delgada, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.molp.2022.12.006","article-title":"Specialized metabolites as versatile tools in shaping plant-microbe associations","volume":"16","author":"Singh","year":"2023","journal-title":"Mol. 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