{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T01:59:42Z","timestamp":1773107982399,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T00:00:00Z","timestamp":1569542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sustainability"],"abstract":"<jats:p>A rich heritage of traditional pear varieties is kept in national Portuguese collections. Out of these varieties, \u201cRocha\u201d dominates national pear production. Although a noticeable phenotypic variation among clones of this variety has been reported, little is known about its genetic variability, as to date molecular studies have been performed on a single \u201cRocha\u201d clone. Eleven Simple Sequence Repeats (SSR) markers were used to assess the genetic diversity of 130 local cultivars, 80 of them being \u201cRocha\u201d clones. The results allowed the differentiation of 75 genotypes of which 29 are \u201cRocha\u201d. Three synonyms groups and four homonymous groups of other local varieties were confirmed. A Bayesian model-based clustering approach identified two distinct clusters. Using flow cytometry, six cultivars were found to be triploids. These results show high genetic variability among \u201cRocha\u201d clones. In conclusion, there is a need for different \u201cRocha\u201d clones to be preserved to enable the correct selection of the multiplication material.<\/jats:p>","DOI":"10.3390\/su11195340","type":"journal-article","created":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T11:14:35Z","timestamp":1569582875000},"page":"5340","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Genetic Diversity and Structure of the Portuguese Pear (Pyrus communis L.) Germplasm"],"prefix":"10.3390","volume":"11","author":[{"given":"\u00c1lvaro","family":"Queiroz","sequence":"first","affiliation":[{"name":"Instituto Polit\u00e9cnico de Viana do Castelo, Escola Superior Agr\u00e1ria, P 4990-706 Ponte de Lima, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9343-4884","authenticated-orcid":false,"given":"Joana","family":"Bagoin Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Unidade de Investiga\u00e7\u00e3o de Biotecnologia e Recursos Gen\u00e9ticos, Quinta do Marqu\u00eas, 2784-505 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8596-7821","authenticated-orcid":false,"given":"Claudia","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3oAgr\u00e1ria e Veterin\u00e1ria, Esta\u00e7\u00e3o Nacional de Fruticultura Vieira de Natividade, 2460-059 Alcoba\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8503-7563","authenticated-orcid":false,"given":"Fernanda","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Unidade de Investiga\u00e7\u00e3o de Biotecnologia e Recursos Gen\u00e9ticos, Quinta do Marqu\u00eas, 2784-505 Oeiras, Portugal"}]},{"given":"Rui","family":"Maia de Sousa","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3oAgr\u00e1ria e Veterin\u00e1ria, Esta\u00e7\u00e3o Nacional de Fruticultura Vieira de Natividade, 2460-059 Alcoba\u00e7a, Portugal"}]},{"given":"Wanda","family":"Viegas","sequence":"additional","affiliation":[{"name":"LEAF, Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1995-095X","authenticated-orcid":false,"given":"Maria Manuela","family":"Veloso","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Unidade de Investiga\u00e7\u00e3o de Biotecnologia e Recursos Gen\u00e9ticos, Quinta do Marqu\u00eas, 2784-505 Oeiras, Portugal"},{"name":"LEAF, Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,27]]},"reference":[{"key":"ref_1","unstructured":"(2019, April 01). 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