{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T12:48:24Z","timestamp":1763642904873,"version":"build-2065373602"},"reference-count":73,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T00:00:00Z","timestamp":1592352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sustainability"],"abstract":"<jats:p>Ruditapes decussatus is a native clam from the Southern Europe and Mediterranean area, relevant to the development of sustainable aquaculture in these regions. As sessile organisms, bivalves are likely to be exposed to chemical contaminations and environmental changes in the aquatic compartment and are widely used as bioindicator species. Carbon-based nanomaterials (CNTs) use is increasing and, consequently, concentrations of these contaminants in aquatic systems will rise. Therefore, it is imperative to assess the potential toxic effects of such compounds and the interactions with environmental factors such as water temperature. For this, we exposed R. decussatus clams to four different water temperatures (10, 15, 20 and 25 \u00b0C) in the presence or absence of CNTs for 96 h. Different parameters related with oxidative stress status, aerobic metabolism, energy reserves and neurotoxicity were evaluated. The relationship and differences among water temperatures and contamination were highlighted by principal coordinates analysis (PCO). CNTs exposure increased oxidative damage as protein carbonylation (PC) in exposed clams at 10 \u00b0C. Higher temperatures (25 \u00b0C) were responsible for the highest redox status (ratio between reduced and oxidized glutathione, GSH\/GSSG) observed as well as neurotoxic effects (acetylcholinesterase\u2014AChE activity). Antioxidant defenses were also modulated by the combination of CNTs exposure with water temperatures, with decrease of glutathione peroxidase (GR) activity at 15 \u00b0C and of glutathione S-transferases (GSTs) activity at 20 \u00b0C, when compared with unexposed clams. Clams energy reserves were not altered, probably due to the short exposure period. Overall, the combined effects of CNTs exposure and increasing water temperatures can impair R. decussatus cellular homeostasis inducing oxidative stress and damage.<\/jats:p>","DOI":"10.3390\/su12124939","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T13:11:32Z","timestamp":1592399492000},"page":"4939","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Environmental Fate of Multistressors on Carpet Shell Clam Ruditapes decussatus: Carbon Nanoparticles and Temperature Variation"],"prefix":"10.3390","volume":"12","author":[{"given":"Lucia","family":"De Marchi","sequence":"first","affiliation":[{"name":"Interuniversity Consortium of Marine Biology of Leghorn \u201cG. Bacci\u201d, 57128 Livorno, Italy"},{"name":"Department of Biology, University of Pisa, Via Derna 1, 56126 Pisa, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4385-6074","authenticated-orcid":false,"given":"Rui Jorge Miranda","family":"Rocha","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM) &amp; Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9839-6290","authenticated-orcid":false,"given":"Andreia C.M.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM) &amp; Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0879-9470","authenticated-orcid":false,"given":"Amadeu M.V.M.","family":"Soares","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM) &amp; Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Carlo","family":"Pretti","sequence":"additional","affiliation":[{"name":"Interuniversity Consortium of Marine Biology of Leghorn \u201cG. Bacci\u201d, 57128 Livorno, Italy"},{"name":"Department of Veterinary Sciences, University of Pisa, 56122 San Piero a Grado (PI), Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4680-9975","authenticated-orcid":false,"given":"Federica","family":"Chiellini","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Industrial Chemistry, University of Pisa, Udr INSTM Pisa, 56126 Pisa, Italy"}]},{"given":"Rosa","family":"Freitas","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM) &amp; Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,17]]},"reference":[{"key":"ref_1","unstructured":"IPCC (2014). 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