{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T17:15:14Z","timestamp":1778001314633,"version":"3.51.4"},"reference-count":34,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T00:00:00Z","timestamp":1643068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BTA-BTA\/30902\/2017"],"award-info":[{"award-number":["PTDC\/BTA-BTA\/30902\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Unit on Applied Molecular Biosciences \u2013 UCIBIO","award":["UIDP\/04378\/2020 and UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020 and UIDB\/04378\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy - i4HB","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sustainability"],"abstract":"<jats:p>The fishing industry produces vast amounts of saline organic side streams that require adequate treatment and disposal. The bioconversion of saline resources into value-added products, such as biodegradable polyhydroxyalkanoates (PHAs), has not yet been fully explored. This study investigated PHA production by mixed microbial cultures under 30 gNaCl\/L, the highest NaCl concentration reported for the acclimatization of a PHA-accumulating mixed microbial culture (MMC). The operational conditions used during the culture-selection stage resulted in an enriched PHA-accumulating culture dominated by the Rhodobacteraceae family (95.2%) and capable of storing PHAs up to 84.1% wt. (volatile suspended solids (VSS) basis) for the highest organic loading rate (OLR) applied (120 Cmmol\/(L.d)). This culture presented a higher preference for the consumption of valeric acid (0.23 \u00b1 0.03 CmolHVal\/(CmolX.h)), and the 3HV monomer polymerization (0.33 \u00b1 0.04 CmmolHV\/(CmmolX.h) was higher as well. As result, a P(3HB-co-3HV)) with high HV content (63% wt.) was produced in the accumulation tests conducted at higher OLRs and with 30 gNaCl\/L. A global volumetric PHA productivity of 0.77 gPHA\/(L.h) and a specific PHA productivity of 0.21 gPHA\/(gX.h) were achieved. These results suggested the significant potential of the bioconversion of saline resources into value-added products, such as PHAs.<\/jats:p>","DOI":"10.3390\/su14031346","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T21:07:11Z","timestamp":1643144831000},"page":"1346","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Polyhydroxyalkanoates Production by Mixed Microbial Culture under High Salinity"],"prefix":"10.3390","volume":"14","author":[{"given":"Jo\u00e3o M.","family":"Carvalho","sequence":"first","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7629-6934","authenticated-orcid":false,"given":"Bruno C.","family":"Marreiros","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4000-1836","authenticated-orcid":false,"given":"Maria A. M.","family":"Reis","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,25]]},"reference":[{"key":"ref_1","unstructured":"(2021, November 29). Fish & Seafood\u2014Worldwide|Statista Market Forecast. Available online: https:\/\/www.statista.com\/outlook\/cmo\/food\/fish-seafood\/worldwide."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ching, Y.C., and Redzwan, G. (2017). Biological treatment of fish processing saline wastewater for reuse as liquid fertilizer. Sustainability, 9.","DOI":"10.3390\/su9071062"},{"key":"ref_3","unstructured":"(2021, May 21). 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