{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:15:51Z","timestamp":1760231751207,"version":"build-2065373602"},"reference-count":67,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology (FCT), Ministry of Science, Technology, and Higher Education (MCTES), European Social Fund (FSE)","award":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"],"award-info":[{"award-number":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"]}]},{"DOI":"10.13039\/501100008530","name":"Integrated Research in Environment, Agro-Chain and Technology","doi-asserted-by":"publisher","award":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"],"award-info":[{"award-number":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Regional Development Fund (ERDF)","award":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"],"award-info":[{"award-number":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"]}]},{"name":"National Funds of FCT","award":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"],"award-info":[{"award-number":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"]}]},{"name":"National Funds of FCT","award":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"],"award-info":[{"award-number":["SFRH\/BD\/141503\/2018","NORTE-01-0145-FEDER-000017","UIDB\/04033\/2020","UIDB\/QUI\/00616\/2020","UIDP\/00616\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sustainability"],"abstract":"<jats:p>The Vilari\u00e7a River was restored in order to improve the fish habitat due to the construction of dams. However, due to the pressure that agriculture exerts on water resources, it is crucial to evaluate its consequences on fish health from a histological perspective. The gonad histopathological changes of two cyprinid species were assessed microscopically and evaluated with semi-quantitative analysis. Histopathological changes in the gonad, gill, and liver were used as biomarkers in the determination of the Integrated Biomarker Response (IBR), as well as to evaluate the histopathological scores between seasons. The observation of the gonad histopathological changes showed that interstitial fibrosis and macrophage aggregates were present exclusively in the Summer and more prevalent in males of large size. In addition, the histopathological scores of the three organs were higher in the Summer. In terms of the severity degree, 98% and 68% of the fish showed pronounced and severe histopathological changes in the gill and liver, respectively, while 28% of the fish showed slight histopathological changes in the gonad. The IBR showed higher values in the Summer and in the middle stream and downstream, which is in agreement with the metal concentrations. Thus, this work showed a relationship between the anomalies present in the fish organs and the quality of the water (classified as polluted). Therefore, minimization measures are presented, such as improving the irrigation methods, preserving the vegetation cover; promoting reforestation in degraded and burned areas; and improving the riparian vegetation.<\/jats:p>","DOI":"10.3390\/su141912596","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:50:01Z","timestamp":1665449401000},"page":"12596","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Combining Multiple Biomarkers to Evaluate the Environmental Stress in Cyprinid Populations"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8454-5400","authenticated-orcid":false,"given":"Regina Maria Bessa","family":"Santos","sequence":"first","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environment and Biological Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"},{"name":"Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8602-7663","authenticated-orcid":false,"given":"Rui Manuel Vitor","family":"Cortes","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environment and Biological Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"},{"name":"Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9486-7160","authenticated-orcid":false,"given":"Lu\u00eds Filipe Sanches","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environment and Biological Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"},{"name":"Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2399-5261","authenticated-orcid":false,"given":"Fernando Ant\u00f3nio Leal","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Chemistry Research Centre, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5038-6085","authenticated-orcid":false,"given":"Simone Da Gra\u00e7a Pinto","family":"Varandas","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environment and Biological Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"},{"name":"Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4026-5965","authenticated-orcid":false,"given":"Sandra Mariza Veiga","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environment and Biological Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"},{"name":"Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, University of Tr\u00e1s-os-Montes and Alto Douro, Ap. 1013, 5001-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.etap.2008.06.001","article-title":"Heavy metals: Implications associated to fish consumption","volume":"26","year":"2008","journal-title":"Environ. 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