{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:52:42Z","timestamp":1774324362293,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T00:00:00Z","timestamp":1658361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CNPq, FAPERJ and CAPES","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"CNPq, FAPERJ and CAPES","award":["C.2020"],"award-info":[{"award-number":["C.2020"]}]},{"name":"Funda\u00e7\u00e3o Carolina","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Funda\u00e7\u00e3o Carolina","award":["C.2020"],"award-info":[{"award-number":["C.2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Technologies"],"abstract":"<jats:p>The increased mortality rates associated with antibiotic resistance has become a significant public health problem worldwide. Living beings produce a variety of endogenous compounds to defend themselves against exogenous pathogens. The knowledge of these endogenous compounds may contribute to the development of improved bioactive ingredients with antimicrobial properties, useful against conventional antibiotic resistance. Cowpea is an herbaceous legume of great interest due to its high protein content and high productivity rates. The study of genetic homology of vicillin (7S) from cowpea (Vigna unguiculata L.) with vicilins from soybean and other beans, such as adzuki, in addition to the need for further studies about potential biological activities of this vegetable, led us to seek the isolation of the vicilin fraction from cowpea and to evaluate the potential in vitro inhibitory action of pathogenic microorganisms. The cowpea beta viginin protein was isolated, characterized, and hydrolyzed in silico and in vitro by two enzymes, namely, pepsin and chymotrypsin. The antimicrobial activity of the protein hydrolysate fractions of cowpea flour was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa, confirming the potential use of the peptides as innovative antimicrobial agents.<\/jats:p>","DOI":"10.3390\/technologies10040089","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T22:38:50Z","timestamp":1658443130000},"page":"89","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Extraction and Characterization of \u03b2-Viginin Protein Hydrolysates from Cowpea Flour as a New Manufacturing Active Ingredient"],"prefix":"10.3390","volume":"10","author":[{"given":"Taline S.","family":"Almeida","sequence":"first","affiliation":[{"name":"Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"},{"name":"University of Tiradentes (Unit), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"}]},{"given":"Caio A.","family":"da Cruz Souza","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Federal University of Bahia, Salvador 40170-115, Brazil"}]},{"given":"Mariana B.","family":"de Cerqueira e Silva","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Federal University of Bahia, Salvador 40170-115, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9844-9313","authenticated-orcid":false,"given":"Fabiana P. R.","family":"Batista","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Federal University of Bahia, Salvador 40170-115, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3558-7019","authenticated-orcid":false,"given":"Ederlan S.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Federal University of Bahia, Salvador 40170-115, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0821-8592","authenticated-orcid":false,"given":"Andr\u00e9 L. S.","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3469-4672","authenticated-orcid":false,"given":"Laura N.","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2976-6264","authenticated-orcid":false,"given":"Carlisson R.","family":"Melo","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"},{"name":"University of Tiradentes (Unit), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"}]},{"given":"Cristiane","family":"Bani","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Sergipe, Aracaju 49100-000, Brazil"}]},{"given":"M. Lucia","family":"Bianconi","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica M\u00e9dica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil"}]},{"given":"Juliana C.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"},{"name":"University of Tiradentes (Unit), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"}]},{"given":"Ricardo L. C.","family":"de Albuquerque-J\u00fanior","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"},{"name":"University of Tiradentes (Unit), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3798-5512","authenticated-orcid":false,"given":"Raquel","family":"de Melo Barbosa","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Rio Grande do Norte, R. Gen. Gustavo Cordeiro de Faria, S\/N-Petr\u00f3polis, Natal 59012-570, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3524-6900","authenticated-orcid":false,"given":"Matheus M.","family":"Pereira","sequence":"additional","affiliation":[{"name":"CICECO-Aveiro Institute of Materials, Departament of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9737-6017","authenticated-orcid":false,"given":"Eliana B.","family":"Souto","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"},{"name":"REQUIMTE\/UCIBIO, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5932-9842","authenticated-orcid":false,"given":"Cleide M. F.","family":"Soares","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"},{"name":"University of Tiradentes (Unit), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6527-6612","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Severino","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"},{"name":"University of Tiradentes (Unit), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/nrd4675","article-title":"Antibiotic resistance breakers: Can repurposed drugs fill the antibiotic discovery void?","volume":"14","author":"Brown","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100065","DOI":"10.1016\/j.afres.2022.100065","article-title":"Recent Findings on the Isolation, Identification and Quantification of Bioactive Peptides","volume":"2","author":"Okoye","year":"2022","journal-title":"Appl. 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