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In animal models exposed to several classes of pesticides, neurotoxic effects have been described, including the reduction of acetylcholinesterase activity in tissue homogenates. However, in homogenates, the reduction in enzymatic activity may also result from lower enzymatic expression and not only from enzymatic inhibition. Thus, in this work, we aimed to investigate the neurotoxic potential of four distinct pesticides: glyphosate (herbicide), imazalil (fungicide), imidacloprid (neonicotinoid insecticide) and lambda-cyhalothrin (pyrethroid insecticide), by assessing their inhibitory effect on the activity of acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase, by using direct in vitro enzymatic inhibition methods. All pesticides dose-dependently inhibited AChE activity, with an inhibition of 11 \u00b1 2% for glyphosate, 48 \u00b1 2% for imidacloprid, 49 \u00b1 3% for imazalil and 50 \u00b1 3% for lambda-cyhalothrin, at 1 mM. Only imazalil inhibited BChE. Imazalil induced dose-dependent inhibition of BChE with identical pattern as that observed for AChE; however, for lower concentrations (up to 500 \u03bcM), imazalil showed higher specificity for AChE, and for higher concentrations, the same specificity was found. Imazalil, at 1 mM, inhibited the activity of BChE by 49 \u00b1 1%. None of the pesticides, up to 1 mM, inhibited tyrosinase activity. In conclusion, the herbicide glyphosate shows specificity for AChE but low inhibitory capacity, the insecticides imidacloprid and \u03bb-cyhalothrin present selective AChE inhibition, while the fungicide IMZ is a broad-spectrum cholinesterase inhibitor capable of inhibiting AChE and BChE in an equal manner. Among these pesticides, the insecticides and the fungicide are the ones with higher neurotoxic potential.<\/jats:p>","DOI":"10.3390\/toxics10080448","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T23:33:01Z","timestamp":1659569581000},"page":"448","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Neurotoxicity Assessment of Four Different Pesticides Using In Vitro Enzymatic Inhibition Assays"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1564-3661","authenticated-orcid":false,"given":"Carlos","family":"Martins-Gomes","sequence":"first","affiliation":[{"name":"Department of Biology and Environment, School of Life Sciences and Environment, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"},{"name":"Center for Research and Technology of Agro-Environmental and Biological Sciences (CITAB-UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-6205","authenticated-orcid":false,"given":"Tiago E.","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Department of Biology and Environment, School of Life Sciences and Environment, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"},{"name":"Center for Research and Technology of Agro-Environmental and Biological Sciences (CITAB-UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5618-1996","authenticated-orcid":false,"given":"T\u00e2nia L.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biology and Environment, School of Life Sciences and Environment, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"},{"name":"Center for Research and Technology of Agro-Environmental and Biological Sciences (CITAB-UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3794-8159","authenticated-orcid":false,"given":"Tatiana","family":"Andreani","sequence":"additional","affiliation":[{"name":"Center for Research and Technology of Agro-Environmental and Biological Sciences (CITAB-UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"},{"name":"GreenUPorto\u2014Sustainable Agrifood Production Research Centre & Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7524-9914","authenticated-orcid":false,"given":"Am\u00e9lia M.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biology and Environment, School of Life Sciences and Environment, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"},{"name":"Center for Research and Technology of Agro-Environmental and Biological Sciences (CITAB-UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,3]]},"reference":[{"key":"ref_1","unstructured":"S\u00edlvio, V. (2019). Toxicological Aspects of Pesticides. Sustainable Agrochemistry: A Compendium of Technologies, Springer International Publishing."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2478\/v10102-009-0001-7","article-title":"Impact of pesticides use in agriculture: Their benefits and hazards","volume":"2","author":"Aktar","year":"2009","journal-title":"Interdiscip. Toxicol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3390\/jox12010003","article-title":"Glyphosate vs. Glyphosate-Based Herbicides Exposure: A Review on Their Toxicity","volume":"12","author":"Silva","year":"2022","journal-title":"J. Xenobiotics"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"402","DOI":"10.3390\/ijerph8051402","article-title":"Pesticide exposure, safety issues, and risk assessment indicators","volume":"8","author":"Damalas","year":"2011","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/j.neuro.2012.05.011","article-title":"Pesticide exposure and Parkinson\u2019s disease: Epidemiological evidence of association","volume":"33","author":"Freire","year":"2012","journal-title":"NeuroToxicology"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Tang, B.L. (2020). Neuropathological Mechanisms Associated with Pesticides in Alzheimer\u2019s Disease. Toxics, 8.","DOI":"10.3390\/toxics8020021"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"32222","DOI":"10.1038\/srep32222","article-title":"Pesticide exposure and risk of Alzheimer\u2019s disease: A systematic review and meta-analysis","volume":"6","author":"Yan","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.envres.2017.10.051","article-title":"Neurotransmitter changes in rat brain regions following glyphosate exposure","volume":"161","author":"Ares","year":"2018","journal-title":"Environ. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e043847","DOI":"10.1002\/alz.043847","article-title":"Glyphosate-based herbicide alters brain amino acid metabolism without affecting blood-brain barrier integrity","volume":"16","author":"Limberger","year":"2020","journal-title":"Alzheimer\u2019s Dement."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"132823","DOI":"10.1016\/j.chemosphere.2021.132823","article-title":"Pesticide sensitivity of Nothobranchius neumanni, a temporary pond predator with a non-generic life-history","volume":"291","author":"Kafula","year":"2021","journal-title":"Chemosphere"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"105414","DOI":"10.1016\/j.envint.2019.105414","article-title":"Use of human neuroblastoma SH-SY5Y cells to evaluate glyphosate-induced effects on oxidative stress, neuronal development and cell death signaling pathways","volume":"135","author":"Maximiliano","year":"2020","journal-title":"Environ. Int."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.chemosphere.2016.03.085","article-title":"The fungicide imazalil induces developmental abnormalities and alters locomotor activity during early developmental stages in zebrafish","volume":"153","author":"Jin","year":"2016","journal-title":"Chemosphere"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.tiv.2017.07.030","article-title":"Comparison of different in vitro cell models for the assessment of pesticide-induced dopaminergic neurotoxicity","volume":"45","author":"Heusinkveld","year":"2017","journal-title":"Toxicol. Vitr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1021\/ar800131p","article-title":"Molecular Recognition of Neonicotinoid Insecticides: The Determinants of Life or Death","volume":"42","author":"Tomizawa","year":"2008","journal-title":"Accounts Chem. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13513","DOI":"10.1021\/acs.jafc.8b05793","article-title":"Imidacloprid Impacts on Neurobehavioral Performance, Oxidative Stress, and Apoptotic Events in the Brain of Adolescent and Adult Rats","volume":"66","author":"Mohammed","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.pestbp.2014.02.007","article-title":"Physiological, biochemical and histological alterations induced by administration of imidacloprid in female albino rats","volume":"110","author":"Vohra","year":"2014","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_17","unstructured":"Krieger, R. (2010). Chapter 95\u2014Imidacloprid: A Neonicotinoid Insecticide. Hayes\u2019 Handbook of Pesticide Toxicology, Academic Press. [3rd ed.]."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.neuint.2014.09.004","article-title":"Evaluation of imidacloprid-induced neurotoxicity in male rats: A protective effect of curcumin","volume":"78","author":"Lonare","year":"2014","journal-title":"Neurochem. Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1016\/j.aspen.2018.07.013","article-title":"The characteristics of voltage-gated sodium channel and the association with lambda cyhalothrin resistance in Spodoptera exigua","volume":"21","author":"Tian","year":"2018","journal-title":"J. Asia-Pacific \u00c8ntomol."},{"key":"ref_20","first-page":"93","article-title":"Neurotoxic effect of lambda-cyhalothrin, a synthetic pyrethroid pesticide: Involvement of oxidative stress and protective role of antioxidant mixture","volume":"9","author":"Ali","year":"2012","journal-title":"NY Sci. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1007\/s12640-012-9313-z","article-title":"Cholinergic Dysfunctions and Enhanced Oxidative Stress in the Neurobehavioral Toxicity of Lambda-Cyhalothrin in Developing Rats","volume":"22","author":"Ansari","year":"2012","journal-title":"Neurotox. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.pestbp.2008.12.005","article-title":"\u03bb-cyhalothrin and cypermethrin induced in vivo alterations in the activity of acetylcholinesterase in a freshwater fish, Channa punctatus (Bloch)","volume":"93","author":"Kumar","year":"2009","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1517\/13543776.2012.701620","article-title":"Acetylcholinesterase inhibitors: A patent review (2008\u2013present)","volume":"22","author":"Pohanka","year":"2012","journal-title":"Expert Opin. Ther. Patents"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1111\/jnc.15332","article-title":"Regulators of cholinergic signaling in disorders of the central nervous system","volume":"158","author":"Winek","year":"2021","journal-title":"J. Neurochem."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Saldanha, C. (2017). Human Erythrocyte Acetylcholinesterase in Health and Disease. Molecules, 22.","DOI":"10.3390\/molecules22091499"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7","DOI":"10.54987\/bessm.v4i1.526","article-title":"Acetylcholinesterase Enzyme (AChE) as a Biosensor and Biomarker for Pesticides: A Mini Review","volume":"4","author":"Umar","year":"2020","journal-title":"Bull. Environ. Sci. Sustain. Manag."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"321213","DOI":"10.1155\/2013\/321213","article-title":"Acetylcholinesterase as a Biomarker in Environmental and Occupational Medicine: New Insights and Future Perspectives","volume":"2013","author":"Lionetto","year":"2013","journal-title":"BioMed Res. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/BF00354093","article-title":"Neurotoxicity of organophosphorus pesticides: Predictions can be based on in vitro studies with hen and human enzymes","volume":"41","author":"Lotti","year":"1978","journal-title":"Arch. Toxicol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ab.2012.06.025","article-title":"Acetylcholinesterase inhibition-based biosensors for pesticide determination: A review","volume":"429","author":"Pundir","year":"2012","journal-title":"Anal. Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1038\/s41467-019-08858-y","article-title":"Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson\u2019s disease pathogenesis","volume":"10","author":"Laguna","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_31","unstructured":"Krieger, R. (2010). Chapter 68\u2014Cholinesterases. Hayes\u2019 Handbook of Pesticide Toxicology, Academic Press. [3rd ed.]."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.2174\/1871527313666141023141545","article-title":"Status of Acetylcholinesterase and Butyrylcholinesterase in Alzheimer\u2019s Disease and Type 2 Diabetes Mellitus","volume":"13","author":"Mushtaq","year":"2014","journal-title":"CNS Neurol. Disord.-Drug Targets"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"315","DOI":"10.2174\/1570159X11311030006","article-title":"Acetylcholinesterase Inhibitors: Pharmacology and Toxicology","volume":"11","author":"Colovic","year":"2013","journal-title":"Curr. Neuropharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/nrn1035","article-title":"Neurobiology of butyrylcholinesterase","volume":"4","author":"Darvesh","year":"2003","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"PCC.12r01412","DOI":"10.4088\/PCC.12r01412","article-title":"A Review of Butyrylcholinesterase as a Therapeutic Target in the Treatment of Alzheimer\u2019s Disease","volume":"15","author":"Nordberg","year":"2013","journal-title":"Prim. Care Companion CNS Disord."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.cbi.2010.01.042","article-title":"Acetylcholinesterase: From 3D structure to function","volume":"187","author":"Dvir","year":"2010","journal-title":"Chem. Interact."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5923","DOI":"10.1016\/j.bmc.2013.05.063","article-title":"NMR determination of Electrophorus electricus acetylcholinesterase inhibition and reactivation by neutral oximes","volume":"21","author":"Soares","year":"2013","journal-title":"Bioorganic Med. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"De Boer, D., Nguyen, N., Mao, J., Moore, J., and Sorin, E. (2021). A Comprehensive Review of Cholinesterase Modeling and Simulation. Biomolecules, 11.","DOI":"10.3390\/biom11040580"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1080\/14756360701421294","article-title":"Acetylcholinesterases\u2014The structural similarities and differences","volume":"22","author":"Wiesner","year":"2007","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1080\/105172399242573","article-title":"Purification and determination of the amino acid sequence of equine serum butyrylcholinesterase","volume":"9","author":"Moorad","year":"1999","journal-title":"Toxicol. Methods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1111\/j.1471-4159.2005.03019.x","article-title":"Tyrosinase exacerbates dopamine toxicity but is not genetically associated with Parkinson\u2019s disease","volume":"93","author":"Greggio","year":"2005","journal-title":"J. Neurochem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid colorimetric determination of acetylcholinesterase activity","volume":"7","author":"Ellman","year":"1961","journal-title":"Biochem. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3617","DOI":"10.1039\/C8FO00456K","article-title":"Thymus pulegioides L. as a rich source of antioxidant, anti-proliferative and neuroprotective phenolic compounds","volume":"9","author":"Taghouti","year":"2018","journal-title":"Food Funct."},{"key":"ref_44","unstructured":"Pratyoosh, S. (2019). Chapter 1\u2014Microbial Tyrosinases: A Novel Enzyme, Structural Features, and Applications. Applied Microbiology and Bioengineering, Academic Press."},{"key":"ref_45","unstructured":"Sam, C., and Bordoni, B. (2022). Physiology, Acetylcholine. StatPearls, StatPearls Publishing."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3675","DOI":"10.1016\/j.chroma.2011.04.021","article-title":"Rapid determination of glyphosate, glufosinate, bialaphos, and their major metabolites in serum by liquid chromatography-tandem mass spectrometry using hydrophilic interaction chromatography","volume":"1218","author":"Yoshioka","year":"2011","journal-title":"J. Chromatogr. A"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"112670","DOI":"10.1016\/j.fct.2021.112670","article-title":"A comparative evaluation of dietary exposure to glyphosate resulting from recommended U.S. diets","volume":"158","author":"Louie","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.chroma.2019.05.013","article-title":"Field-amplified sample injection and sweeping micellar electrokinetic chromatography in analysis of glyphosate and aminomethylphosphonic acid in wheat","volume":"1601","author":"Gotti","year":"2019","journal-title":"J. Chromatogr. A"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1080\/19440049.2015.1086824","article-title":"Follow-up of the fate of imazalil from post-harvest lemon surface treatment to a baking experiment","volume":"32","author":"Vass","year":"2015","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1002\/etc.2104","article-title":"Analysis of imidacloprid residues in fruits, vegetables, cereals, fruit juices, and baby foods, and daily intake estimation in and around Lucknow, India","volume":"32","author":"Kapoor","year":"2012","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Silva, A.M., Martins-Gomes, C., Silva, T.L., Coutinho, T.E., Souto, E.B., and Andreani, T. (2022). In Vitro Assessment of Pesticides Toxicity and Data Correlation with Pesticides Physicochemical Properties for Prediction of Toxicity in Gastrointestinal and Skin Contact Exposure. Toxics, 10.","DOI":"10.3390\/toxics10070378"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Silva, A.M., Martins-Gomes, C., Ferreira, S.S., Souto, E.B., and Andreani, T. (2022). Molecular Physicochemical Properties of Selected Pesticides as Predictive Factors for Oxidative Stress and Apoptosis-Dependent Cell Death in Caco-2 and HepG2 Cells. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23158107"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.ecoenv.2014.05.025","article-title":"Biochemical effects of glyphosate based herbicide, Excel Mera 71 on enzyme activities of acetylcholinesterase (AChE), lipid peroxidation (LPO), catalase (CAT), glutathione-S-transferase (GST) and protein content on teleostean fishes","volume":"107","author":"Samanta","year":"2014","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.chemosphere.2015.03.042","article-title":"Roundup\u00ae exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum","volume":"135","author":"Duarte","year":"2015","journal-title":"Chemosphere"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.ecoenv.2005.07.017","article-title":"Effect of glyphosate herbicide on acetylcholinesterase activity and metabolic and hematological parameters in piava (Leporinus obtusidens)","volume":"65","author":"Glusczak","year":"2006","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ecoenv.2013.09.011","article-title":"Effect of a glyphosate-based herbicide in Cyprinus carpio: Assessment of acetylcholinesterase activity, hematological responses and serum biochemical parameters","volume":"98","author":"Mirvaghefi","year":"2013","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1016\/j.etap.2014.04.008","article-title":"The effect of glyphosate, its metabolites and impurities on erythrocyte acetylcholinesterase activity","volume":"37","author":"Kwiatkowska","year":"2014","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1081\/PFC-100000914","article-title":"Influence of paraquat, glyphosate, and cadmium on the activity of some serum enzymes and protein electrophoretic behavior (in vitro)","volume":"36","author":"Yousef","year":"2001","journal-title":"J. Environ. Sci. Health Part B"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.2169\/internalmedicine.2562-18","article-title":"Decrease in Butyrylcholinesterase Accompanied by Intermediate-like Syndrome after Massive Ingestion of a Glyphosate-surfactant","volume":"58","author":"Takeuchi","year":"2019","journal-title":"Intern. Med."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2030","DOI":"10.1016\/j.matpr.2021.04.281","article-title":"Mitigatory role of butyrylcholinesterase in freshwater fish Labeo rohita exposed to glyphosate based herbicide Roundup\u00ae","volume":"47","author":"Geetha","year":"2021","journal-title":"Mater. Today Proc."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1007\/s00244-010-9578-2","article-title":"Toxicity of Four Herbicide Formulations with Glyphosate on Rhinella arenarum (Anura: Bufonidae) Tadpoles: B-esterases and Glutathione S-transferase Inhibitors","volume":"60","author":"Lajmanovich","year":"2010","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0009-2797(99)00014-9","article-title":"Differences in active-site gorge dimensions of cholinesterases revealed by binding of inhibitors to human butyrylcholinesterase","volume":"119\u2013120","author":"Saxena","year":"1999","journal-title":"Chem. Interact."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"109287","DOI":"10.1016\/j.cbi.2020.109287","article-title":"Effects of low-level imidacloprid oral exposure on cholinesterase activity, oxidative stress responses, and primary DNA damage in the blood and brain of male Wistar rats","volume":"338","author":"Kopjar","year":"2021","journal-title":"Chem.-Biol. Interact."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.chemosphere.2017.02.047","article-title":"Neurotoxic responses in brain tissues of rainbow trout exposed to imidacloprid pesticide: Assessment of 8-hydroxy-2-deoxyguanosine activity, oxidative stress and acetylcholinesterase activity","volume":"175","author":"Topal","year":"2017","journal-title":"Chemosphere"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"106050","DOI":"10.1016\/j.aquatox.2021.106050","article-title":"Assessment of acetylcholinesterase, catalase, and glutathione S-transferase as biomarkers for imidacloprid exposure in penaeid shrimp","volume":"242","author":"Butcherine","year":"2021","journal-title":"Aquat. Toxicol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"634.e1","DOI":"10.1016\/j.ajem.2007.09.024","article-title":"Fatal intoxication with imidacloprid insecticide","volume":"26","author":"Shadnia","year":"2008","journal-title":"Am. J. Emerg. Med."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.forsciint.2005.04.015","article-title":"Two fatal intoxication cases with imidacloprid: LC\/MS analysis","volume":"153","author":"Teixeira","year":"2005","journal-title":"Forensic Sci. Int."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"105785","DOI":"10.1016\/j.envint.2020.105785","article-title":"Assessment of imidacloprid related exposure using imidacloprid-olefin and desnitro-imidacloprid: Neonicotinoid insecticides in human urine in Wuhan, China","volume":"141","author":"Wang","year":"2020","journal-title":"Environ. Int."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.scitotenv.2014.08.051","article-title":"Quantification of neonicotinoid insecticide residues in soils from cocoa plantations using a QuEChERS extraction procedure and LC-MS\/MS","volume":"499","author":"Dankyi","year":"2014","journal-title":"Sci. Total Environ."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Kittelmann, A., M\u00fcller, C., Rohn, S., and Michalski, B. (2022). Transfer of Pesticide Residues from Grapes (Vitis vinifera) into Wine\u2014Correlation with Selected Physicochemical Properties of the Active Substances. Toxics, 10.","DOI":"10.3390\/toxics10050248"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1080\/12265071.2003.9647700","article-title":"Effect of lambda cyhalothrin (pyrethroid) and monocrotophos (organophosphate) on cholinesterase activity in liver, kidney and brain of Rana cyanophlyctis","volume":"7","author":"Khan","year":"2003","journal-title":"Korean J. Biol. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1096\/fj.02-0736com","article-title":"Tyrosinase: A developmentally specific major determinant of peripheral dopamine","volume":"17","author":"Eisenhofer","year":"2003","journal-title":"FASEB J."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Smith, D.F.Q., Camacho, E., Thakur, R., Barron, A.J., Dong, Y., Dimopoulos, G., Broderick, N.A., and Casadevall, A. (2021). Glyphosate inhibits melanization and increases susceptibility to infection in insects. PLoS Biol., 19.","DOI":"10.1371\/journal.pbio.3001182"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"4860","DOI":"10.1021\/jf200485k","article-title":"Neonicotinoid Insecticides: Oxidative Stress in Planta and Metallo-oxidase Inhibition","volume":"59","author":"Ford","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1177\/0748233710375951","article-title":"Toxicologic evaluation of imazalil with particular reference to genotoxic and teratogenic potentials","volume":"26","year":"2010","journal-title":"Toxicol. Ind. Health"},{"key":"ref_76","first-page":"111","article-title":"In Vitro Inhibition of the Diphenolase Activity of Tyrosinase by Insecticides and Allelochemicals in Micromelalopha troglodyta (Lepidoptera: Notodontidae)","volume":"44","author":"Tang","year":"2009","journal-title":"J. \u00c8ntomol. Sci."}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/10\/8\/448\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:03:41Z","timestamp":1760141021000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/10\/8\/448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,3]]},"references-count":76,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["toxics10080448"],"URL":"https:\/\/doi.org\/10.3390\/toxics10080448","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,3]]}}}