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(MCIN)","award":["FCOMP-01-0124-FEDER-019321"],"award-info":[{"award-number":["FCOMP-01-0124-FEDER-019321"]}]},{"name":"Spanish Ministry of Science and Innovation (MCIN)","award":["FCT PTDC\/AAC-AMB\/117178\/2010"],"award-info":[{"award-number":["FCT PTDC\/AAC-AMB\/117178\/2010"]}]},{"name":"Spanish Ministry of Science and Innovation (MCIN)","award":["BD\/UI88\/5534\/2011"],"award-info":[{"award-number":["BD\/UI88\/5534\/2011"]}]},{"name":"Spanish Ministry of Science and Innovation (MCIN)","award":["RYC2020-029322-I"],"award-info":[{"award-number":["RYC2020-029322-I"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>The world population is experiencing colossal growth and thus demand for food, leading to an increase in the use of pesticides. Persistent pesticide contamination, such as carbendazim, remains a pressing environmental concern, with potentially long-term impacts on aquatic ecosystems. In the present study, Daphnia magna was exposed to carbendazim (5 \u00b5g L\u22121) for 12 generations, with the aim of assessing gene transcription alterations induced by carbendazim (using a D. magna custom microarray). The results showed that carbendazim caused changes in genes involved in the response to stress, DNA replication\/repair, neurotransmission, ATP production, and lipid and carbohydrate metabolism at concentrations already found in the environment. These outcomes support the results of previous studies, in which carbendazim induced genotoxic effects and reproduction impairment (increasing the number of aborted eggs with the decreasing number of neonates produced). The exposure of daphnids to carbendazim did not cause a stable change in gene transcription between generations, with more genes being differentially expressed in the F0 generation than in the F12 generation. This could show some possible daphnid acclimation after 12 generations and is aligned with previous multigenerational studies where few ecotoxicological effects at the individual and populational levels and other subcellular level effects (e.g., biochemical biomarkers) were found.<\/jats:p>","DOI":"10.3390\/toxics11110918","type":"journal-article","created":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T01:17:51Z","timestamp":1699579071000},"page":"918","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Daphnia magna Multigeneration Exposure to Carbendazim: Gene Transcription Responses"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0689-639X","authenticated-orcid":false,"given":"Ana Rita R.","family":"Silva","sequence":"first","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM) & Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Patr\u00edcia V.","family":"Silva","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM) & Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4000-7461","authenticated-orcid":false,"given":"Ana Raquel","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences & Institute for Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0494-4266","authenticated-orcid":false,"given":"M. Nazaret","family":"Gonz\u00e1lez-Alcaraz","sequence":"additional","affiliation":[{"name":"Department of Agricultural Engineering of the E.T.S.I.A., Technical University of Cartagena, 30203 Cartagena, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5651-0208","authenticated-orcid":false,"given":"Cornelis A. M.","family":"van Gestel","sequence":"additional","affiliation":[{"name":"Amsterdam Institute for Life and Environment (A-LIFE), Faculty of Science, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3954-3590","authenticated-orcid":false,"given":"Dick","family":"Roelofs","sequence":"additional","affiliation":[{"name":"Amsterdam Institute for Life and Environment (A-LIFE), Faculty of Science, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands"},{"name":"Keygene N.V., Agro Business Park 90, 6708 PW Wageningen, The Netherlands"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2243-6123","authenticated-orcid":false,"given":"Gabriela","family":"Moura","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences & Institute for Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0879-9470","authenticated-orcid":false,"given":"Amadeu M. V. M.","family":"Soares","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM) & Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5393-9623","authenticated-orcid":false,"given":"Susana","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM) & Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"13809","DOI":"10.1038\/srep13809","article-title":"Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications","volume":"5","author":"Campos","year":"2015","journal-title":"Sci. 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