{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T19:58:07Z","timestamp":1772740687523,"version":"3.50.1"},"reference-count":86,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,9]],"date-time":"2021-01-09T00:00:00Z","timestamp":1610150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/144904\/2019"],"award-info":[{"award-number":["SFRH\/BD\/144904\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>The improper use of synthetic fungicides has raised public concerns related to environmental pollution and animal health. Over the years, plant-derived antifungals have been investigated as safer alternatives, although little scientific evidence of its neurodevelopmental effects exist. The main objective of this study was to explore the effects of three alternative natural extracts (Equisetum arvense, Mimosa tenuiflora, Thymol) with antifungal properties during the early development of zebrafish by evaluating different teratogenic, oxidative stress and behavioural outcomes. Following the determination of the 96 h-LC50, exposure to sublethal concentrations showed the safety profile of both E. arvense and M. tenuiflora. However, following 96-h exposure to Thymol, increased lethality, pericardial oedema, yolk and eye deformations, and decreased body length were observed. The reduced and oxidized glutathione (GSH:GSSG) ratio was increased, and the glutathione-s-transferase activity in the group exposed to the highest Thymol concentration. Overall, these results support a more reducing environment associated with possible effects at the cellular proliferation level. In addition, the disruption of behavioural states (fear- and anxiety-like disorders) were noted, pointing to alterations in the c-Jun N-terminal kinase developmental signalling pathway, although further studies are required to explore this rationale. Notwithstanding, the results provide direct evidence of the teratogenic effects of Thymol, which might have consequences for non-target species.<\/jats:p>","DOI":"10.3390\/toxics9010008","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T23:03:42Z","timestamp":1610319822000},"page":"8","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Teratogenic, Oxidative Stress and Behavioural Outcomes of Three Fungicides of Natural Origin (Equisetum arvense, Mimosa tenuiflora, Thymol) on Zebrafish (Danio rerio)"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8388-5590","authenticated-orcid":false,"given":"Raquel","family":"Vieira","sequence":"first","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Carlos","family":"Ven\u00e2ncio","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Department of Animal Science, School of Agrarian and Veterinary Sciences (ECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9870-6666","authenticated-orcid":false,"given":"Lu\u00eds","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Laboratory Animal Science, IBMC-Instituto de Biologia Molecular Celular, Universidade do Porto, 4200-135 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1002\/ps.4190","article-title":"Progress of modern agricultural chemistry and future prospects","volume":"72","author":"Jeschke","year":"2016","journal-title":"Pest Manag. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1002\/fes3.108","article-title":"Pesticides, environment, and food safety","volume":"6","author":"Carvalho","year":"2017","journal-title":"Food Energy Secur."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.marpolbul.2015.04.029","article-title":"Occurrence of pharmaceutical compounds and pesticides in aquatic systems","volume":"96","author":"Tapie","year":"2015","journal-title":"Mar. Pollut. Bull."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3347","DOI":"10.1021\/acs.est.8b04392","article-title":"Fungicides: An overlooked pesticide class?","volume":"53","author":"Zubrod","year":"2019","journal-title":"Environ. Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Li, Z., and Jennings, A. (2017). Worldwide regulations of standard values of pesticides for human health risk control: A review. Int. J. Environ. Res. Public Health, 14.","DOI":"10.3390\/ijerph14070826"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1080\/09583157.2018.1548574","article-title":"Biofungicides as alternative to synthetic fungicide control of grey mould (Botrytis cinerea)\u2014Prospects and challenges","volume":"29","author":"Abbey","year":"2019","journal-title":"Biocontrol Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1002\/ps.5350","article-title":"Natural products: A strategic lead generation approach in crop protection discovery","volume":"75","author":"Lorsbach","year":"2019","journal-title":"Pest Manag. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.postharvbio.2016.04.017","article-title":"GRAS, plant- and animal-derived compounds as alternatives to conventional fungicides for the control of postharvest diseases of fresh horticultural produce","volume":"122","author":"Palou","year":"2016","journal-title":"Postharvest Biol. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1002\/ps.5433","article-title":"Pesticidal natural products\u2014Status and future potential","volume":"75","author":"Marrone","year":"2019","journal-title":"Pest Manag. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1016\/j.tplants.2016.10.005","article-title":"Essential oils as ecofriendly biopesticides? Challenges and constraints","volume":"21","author":"Pavela","year":"2016","journal-title":"Trends Plant Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Damalas, C.A., and Koutroubas, S.D. (2020). Botanical pesticides for eco-friendly pest management: Drawbacks and limitations. Pesticides in Crop Production: Physiological and Biochemical Action, Wiley.","DOI":"10.1002\/9781119432241.ch10"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.marenvres.2019.04.005","article-title":"Fish facing global change: Are early stages the lifeline?","volume":"147","author":"Vagner","year":"2019","journal-title":"Mar. Environ. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"16","DOI":"10.12924\/of2017.03010016","article-title":"Basic substances under EU pesticide regulation: An opportunity for organic production?","volume":"3","author":"Marchand","year":"2017","journal-title":"Org. Farming"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/1752-1947-5-129","article-title":"Prenatal exposure of a girl with autism spectrum disorder to \u2018horsetail\u2019 (Equisetum arvense) herbal remedy and alcohol: A case report","volume":"5","author":"Angulo","year":"2011","journal-title":"J. Med. Case Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.toxicon.2007.06.012","article-title":"Teratogenicity of Mimosa tenuiflora seeds to pregnant rats","volume":"51","author":"Medeiros","year":"2008","journal-title":"Toxicon"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1354\/vp.44-6-928","article-title":"Mimosa tenuiflora as a cause of malformations in ruminants in the northeastern Brazilian semiarid rangelands","volume":"44","author":"Pimentel","year":"2007","journal-title":"Vet. Pathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.foodchem.2016.04.111","article-title":"Antibacterial and antifungal activities of thymol: A brief review of the literature","volume":"210","author":"Marchese","year":"2016","journal-title":"Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0041-008X(80)90298-7","article-title":"Toxicity and teratogenicity of food additive chemicals in the developing chicken embryo","volume":"56","author":"Verrett","year":"1980","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.etap.2018.11.001","article-title":"Thymol exposure mediates pro-oxidant shift by regulating Nrf2 and apoptotic events in zebrafish (Danio rerio) embryos","volume":"65","author":"Krishnan","year":"2019","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11280","DOI":"10.1038\/s41598-020-68188-8","article-title":"Chemical composition and anti-oxidant potential on essential oils of Thymus quinquecostatus Celak. from Loess Plateau in China, regulating Nrf2\/Keap1 signaling pathway in zebrafish","volume":"10","author":"He","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Capatina, L., Todirascu-Ciornea, E., Napoli, E.M., Ruberto, G., Hritcu, L., and Dumitru, G. (2020). Thymus vulgaris essential oil protects zebrafish against cognitive dysfunction by regulating cholinergic and antioxidants systems. Antioxidants, 9.","DOI":"10.3390\/antiox9111083"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2248","DOI":"10.1002\/jsfa.6033","article-title":"Equisetum arvense hydro-alcoholic extract: Phenolic composition and antifungal and antimycotoxigenic effect against Aspergillus flavus and Fusarium verticillioides in stored maize","volume":"93","author":"Garcia","year":"2013","journal-title":"J. Sci. Food Agric."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1007\/s10658-018-01629-3","article-title":"Extract of Mimosa tenuiflora and Quercus robur as potential eco-friendly management tool against Sclerotinia sclerotiorum in Lactuca sativa enhancing the natural plant defences","volume":"153","author":"Llorens","year":"2019","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.marpolbul.2005.07.020","article-title":"Resolving mechanisms of toxicity while pursuing ecotoxicological relevance?","volume":"51","author":"Hinton","year":"2005","journal-title":"Mar. Pollut. Bull."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/bs.ctdb.2016.10.007","article-title":"Zebrafish in toxicology and environmental health","volume":"124","author":"Bambino","year":"2017","journal-title":"Curr. Top. Dev. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"12","DOI":"10.19040\/ecocycles.v4i2.108","article-title":"Recent advances and future trends in zebrafish bioassays for aquatic ecotoxicology","volume":"4","author":"Magyary","year":"2018","journal-title":"Ecocycles"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1007\/s11356-008-0018-z","article-title":"The zebrafish embryo model in environmental risk assessment\u2014Applications beyond acute toxicity testing","volume":"15","author":"Scholz","year":"2008","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s40572-014-0029-5","article-title":"Zebrafish: A marvel of high-throughput biology for 21(st) century toxicology","volume":"1","author":"Bugel","year":"2014","journal-title":"Curr. Environ. Health Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.cbpc.2010.05.010","article-title":"Biomarkers as a tool to assess effects of chromium (VI): Comparison of responses in zebrafish early life stages and adults","volume":"152","author":"Domingues","year":"2010","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1002\/tox.20550","article-title":"Early life stage and genetic toxicity of stannous chloride on zebrafish embryos and adults: Toxic effects of tin on zebrafish","volume":"26","author":"Sisman","year":"2011","journal-title":"Environ. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"9690","DOI":"10.1021\/es301729q","article-title":"Predicting adult fish acute lethality with the zebrafish embryo: Relevance of test duration, endpoints, compound properties, and exposure concentration analysis","volume":"46","author":"Knobel","year":"2012","journal-title":"Environ. Sci. Technol."},{"key":"ref_32","unstructured":"OECD Guidelines (2013). Test No. 236: Fish Embryo Acute Toxicity (FET) Test. OECD Guidelines for the Testing of Chemicals, Section 2, OECD."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1021\/acs.chemrestox.6b00122","article-title":"Embryonic stage-dependent teratogenicity of ketamine in zebrafish (Danio rerio)","volume":"29","author":"Felix","year":"2016","journal-title":"Chem. Res. Toxicol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.taap.2017.02.013","article-title":"Morphological and behavioral responses of zebrafish after 24 h of ketamine embryonic exposure","volume":"321","author":"Felix","year":"2017","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"21174","DOI":"10.1007\/s11356-020-08412-0","article-title":"Toxic effects of a mancozeb-containing commercial formulation at environmental relevant concentrations on zebrafish embryonic development","volume":"27","author":"Vieira","year":"2020","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2721","DOI":"10.1007\/s11356-012-1359-1","article-title":"A novel \u201cIntegrated Biomarker Response\u201d calculation based on reference deviation concept","volume":"20","author":"Sanchez","year":"2013","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1007\/s00213-016-4491-7","article-title":"Behavioral alterations of zebrafish larvae after early embryonic exposure to ketamine","volume":"234","author":"Felix","year":"2017","journal-title":"Psychopharmacology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.aquatox.2015.11.017","article-title":"Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos","volume":"170","author":"Andrade","year":"2016","journal-title":"Aquat. Toxicol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Chahardehi, A.M., Arsad, H., and Lim, V. (2020). Zebrafish as a successful animal model for screening toxicity of medicinal plants. Plants, 9.","DOI":"10.3390\/plants9101345"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"29971","DOI":"10.1007\/s11356-018-1320-z","article-title":"Chemical composition and antifungal activity of plant extracts traditionally used in organic and biodynamic farming","volume":"25","author":"Andreu","year":"2018","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/S0959-437X(00)00209-4","article-title":"Axis formation and patterning in zebrafish","volume":"11","author":"Schier","year":"2001","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/nrendo.2016.12","article-title":"BMP signalling in skeletal development, disease and repair","volume":"12","author":"Salazar","year":"2016","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"dmm037051","DOI":"10.1242\/dmm.037051","article-title":"Wnt signaling in orofacial clefts: Crosstalk, pathogenesis and models","volume":"12","author":"Reynolds","year":"2019","journal-title":"Dis. Model. Mech."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2895","DOI":"10.4314\/tjpr.v16i12.13","article-title":"Thymol inhibits cell migration and invasion by downregulating the activation of PI3K\/AKT and ERK pathways in human colon cancer cells","volume":"16","author":"Lv","year":"2017","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4710","DOI":"10.1038\/s41467-019-12609-4","article-title":"Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo","volume":"10","author":"Akieda","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"a007955","DOI":"10.1101\/cshperspect.a007955","article-title":"Wnt signaling in vertebrate axis specification","volume":"5","author":"Hikasa","year":"2013","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3050","DOI":"10.1242\/dev.137075","article-title":"Proliferation, survival and metabolism: The role of PI3K\/AKT\/mTOR signalling in pluripotency and cell fate determination","volume":"143","author":"Yu","year":"2016","journal-title":"Development"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1042\/BC20090034","article-title":"Altering PI3K-Akt signalling in zebrafish embryos affects PTEN phosphorylation and gastrulation","volume":"101","author":"Finkielsztein","year":"2009","journal-title":"Biol. Cell"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3389\/fonc.2018.00160","article-title":"Phosphoinositide 3-Kinase\/Akt signaling and redox metabolism in cancer","volume":"8","author":"Koundouros","year":"2018","journal-title":"Front. Oncol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.freeradbiomed.2015.06.022","article-title":"Nrf2 and Nrf2-related proteins in development and developmental toxicity: Insights from studies in zebrafish (Danio rerio)","volume":"88","author":"Hahn","year":"2015","journal-title":"Free Radic. Biol. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"48021","DOI":"10.1074\/jbc.M308439200","article-title":"Deficiency of the Nrf1 and Nrf2 transcription factors results in early embryonic lethality and severe oxidative stress","volume":"278","author":"Leung","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.freeradbiomed.2010.07.011","article-title":"Oxidative stress in development: Nature or nurture?","volume":"49","author":"Dennery","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.chemosphere.2016.11.004","article-title":"Glutathione and zebrafish: Old assays to address a current issue","volume":"168","author":"Massarsky","year":"2017","journal-title":"Chemosphere"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.freeradbiomed.2013.06.011","article-title":"Glutathione redox dynamics and expression of glutathione-related genes in the developing embryo","volume":"65","author":"Goldstone","year":"2013","journal-title":"Free Radic. Biol. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1093\/toxsci\/kfx293","article-title":"Glutathione S-Transferase protein expression in different life stages of zebrafish (Danio rerio)","volume":"162","author":"Tierbach","year":"2018","journal-title":"Toxicol. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/S0891-5849(01)00480-4","article-title":"Redox environment of the cell as viewed through the redox state of the glutathione disulfide\/glutathione couple","volume":"30","author":"Schafer","year":"2001","journal-title":"Free Radic. Biol. Med."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Liu, Y., Sepich, D.S., and Solnica-Krezel, L. (2017). Stat3\/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genet., 13.","DOI":"10.1371\/journal.pgen.1006564"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.freeradbiomed.2015.06.030","article-title":"Roles of Nrf2 in cell proliferation and differentiation","volume":"88","author":"Murakami","year":"2015","journal-title":"Free Radic. Biol. Med."},{"key":"ref_59","first-page":"1557","article-title":"A comparative study of the antioxidant\/prooxidant effects of carvacrol and thymol at various concentrations on membrane and DNA of parental and drug resistant H1299 cells","volume":"7","author":"Ozkan","year":"2012","journal-title":"Nat. Prod. Commun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1080\/13813455.2018.1476979","article-title":"Effects of thymol and carvacrol on sperm quality and oxidant\/antioxidant balance in rats","volume":"125","author":"Guvenc","year":"2019","journal-title":"Arch. Physiol. Biochem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.neuint.2010.01.004","article-title":"Glutathione depletion causes a JNK and p38MAPK-mediated increase in expression of cystathionine-gamma-lyase and upregulation of the transsulfuration pathway in C6 glioma cells","volume":"56","author":"Kandil","year":"2010","journal-title":"Neurochem. Int."},{"key":"ref_62","first-page":"425","article-title":"Cell proliferation, reactive oxygen and cellular glutathione","volume":"3","author":"Day","year":"2006","journal-title":"Dose Response"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1042\/bj3250183","article-title":"Glutathione and the rate of cellular proliferation determine tumour cell sensitivity to tumour necrosis factor in vivo","volume":"325","author":"Obrador","year":"1997","journal-title":"Biochem. J."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.aquatox.2014.10.013","article-title":"Characterization of glutathione-S-transferases in zebrafish (Danio rerio)","volume":"158","author":"Glisic","year":"2015","journal-title":"Aquat. Toxicol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"104567","DOI":"10.1016\/j.pestbp.2020.104567","article-title":"Comparison of sublethal effects of natural acaricides carvacrol and thymol on honeybees","volume":"166","author":"Glavan","year":"2020","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1089\/jmf.2005.8.184","article-title":"Thyme (Thymus vulgaris L.) leaves and its constituents increase the activities of xenobiotic-metabolizing enzymes in mouse liver","volume":"8","author":"Sasaki","year":"2005","journal-title":"J. Med. Food"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1007\/s10340-014-0602-6","article-title":"Toxicity of essential oil compounds against diamondback moth, Plutella xylostella, and their impact on detoxification enzyme activities","volume":"87","author":"Kumrungsee","year":"2014","journal-title":"J. Pest Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.pestbp.2018.06.015","article-title":"Effects of alpha-pinene, trans-anethole, and thymol as the essential oil constituents on antioxidant system and acetylcholine esterase of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)","volume":"150","author":"Shahriari","year":"2018","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1038\/cdd.2010.80","article-title":"Glutathione transferases as mediators of signaling pathways involved in cell proliferation and cell death","volume":"17","author":"Laborde","year":"2010","journal-title":"Cell Death Differ."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1007\/s10126-013-9520-5","article-title":"Involvement of JNK in the embryonic development and organogenesis in zebrafish","volume":"15","author":"Xiao","year":"2013","journal-title":"Mar. Biotechnol. (NY)"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1002\/jat.1708","article-title":"Exposure to the JNK inhibitor SP600125 (anthrapyrazolone) during early zebrafish development results in morphological defects","volume":"33","author":"Valesio","year":"2013","journal-title":"J. Appl. Toxicol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1007\/s10753-013-9732-x","article-title":"Thymol inhibits LPS-stimulated inflammatory response via down-regulation of NF-kappaB and MAPK signaling pathways in mouse mammary epithelial cells","volume":"37","author":"Liang","year":"2014","journal-title":"Inflammation"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"157","DOI":"10.3109\/1547691X.2015.1029145","article-title":"Modulatory effects of thymol and carvacrol on inflammatory transcription factors in lipopolysaccharide-treated macrophages","volume":"13","author":"Gholijani","year":"2016","journal-title":"J. Immunotoxicol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1515\/rns.2011.008","article-title":"Imaging escape and avoidance behavior in zebrafish larvae","volume":"22","author":"Colwill","year":"2011","journal-title":"Rev. Neurosci."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/dneu.20873","article-title":"Fear, anxiety, and control in the zebrafish","volume":"72","author":"Jesuthasan","year":"2012","journal-title":"Dev. Neurobiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.1111\/jphp.13162","article-title":"Thymol reverses depression-like behaviour and upregulates hippocampal BDNF levels in chronic corticosterone-induced depression model in female mice","volume":"71","author":"Capibaribe","year":"2019","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.bbr.2015.04.052","article-title":"Thymol produces an antidepressant-like effect in a chronic unpredictable mild stress model of depression in mice","volume":"291","author":"Deng","year":"2015","journal-title":"Behav. Brain Res."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1002\/dneu.20913","article-title":"Genetic dissection of the zebrafish habenula, a possible switching board for selection of behavioral strategy to cope with fear and anxiety","volume":"72","author":"Okamoto","year":"2012","journal-title":"Dev. Neurobiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1038\/nn.2654","article-title":"The habenula is crucial for experience-dependent modification of fear responses in zebrafish","volume":"13","author":"Agetsuma","year":"2010","journal-title":"Nat. Neurosci."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1016\/j.cub.2010.11.025","article-title":"The habenula prevents helpless behavior in larval zebrafish","volume":"20","author":"Lee","year":"2010","journal-title":"Curr. Biol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"eaaz3173","DOI":"10.1126\/sciadv.aaz3173","article-title":"Functional properties of habenular neurons are determined by developmental stage and sequential neurogenesis","volume":"6","author":"Fore","year":"2020","journal-title":"Sci. Adv."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1749-8104-8-3","article-title":"Neurogenesis in zebrafish\u2014From embryo to adult","volume":"8","author":"Schmidt","year":"2013","journal-title":"Neural Dev."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"10791","DOI":"10.1128\/MCB.25.24.10791-10802.2005","article-title":"Inhibited neurogenesis in JNK1-deficient embryonic stem cells","volume":"25","author":"Amura","year":"2005","journal-title":"Mol. Cell. Biol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"5856","DOI":"10.1007\/s12035-019-1476-7","article-title":"JNK Isoforms Are Involved in the Control of Adult Hippocampal Neurogenesis in Mice, Both in Physiological Conditions and in an Experimental Model of Temporal Lobe Epilepsy","volume":"56","author":"Landa","year":"2019","journal-title":"Mol. Neurobiol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/nrn3729","article-title":"Nuclear and cytosolic JNK signalling in neurons","volume":"15","author":"Coffey","year":"2014","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3233\/BPL-170062","article-title":"JNK Regulation of depression and anxiety","volume":"3","author":"Hollos","year":"2018","journal-title":"Brain Plast."}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/9\/1\/8\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:09:14Z","timestamp":1760159354000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/9\/1\/8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,9]]},"references-count":86,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,1]]}},"alternative-id":["toxics9010008"],"URL":"https:\/\/doi.org\/10.3390\/toxics9010008","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,9]]}}}