{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:30:59Z","timestamp":1772253059525,"version":"3.50.1"},"reference-count":93,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2018,5,5]],"date-time":"2018-05-05T00:00:00Z","timestamp":1525478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/113752\/2015"],"award-info":[{"award-number":["SFRH\/BD\/113752\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/91518\/2012"],"award-info":[{"award-number":["SFRH\/BPD\/91518\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>The Cape Verde islands are part of the African Sahelian arid belt that possesses an erratic rain pattern prompting the need for water reservoirs, which are now critical for the country\u2019s sustainability. Worldwide, freshwater cyanobacterial blooms are increasing in frequency due to global climate change and the eutrophication of water bodies, particularly in reservoirs. To date, there have been no risk assessments of cyanobacterial toxin production in these man-made structures. We evaluated this potential risk using 16S rRNA gene amplicon sequencing and full metagenome sequencing in freshwater reservoirs of Cape Verde. Our analysis revealed the presence of several potentially toxic cyanobacterial genera in all sampled reservoirs. Faveta potentially toxic and bloom-forming Microcystis sp., dominated our samples, while a Cryptomonas green algae and Gammaproteobacteria dominated Saquinho and Poil\u00e3o reservoirs. We reconstructed and assembled the Microcystis genome, extracted from the metagenome of bulk DNA from Faveta water. Phylogenetic analysis of Microcystis cf. aeruginosa CV01\u2019s genome revealed its close relationship with other Microcystis genomes, as well as clustering with other continental African strains, suggesting geographical coherency. In addition, it revealed several clusters of known toxin-producing genes. This survey reinforces the need to better understand the country\u2019s microbial ecology as a whole of water reservoirs on the rise.<\/jats:p>","DOI":"10.3390\/toxins10050186","type":"journal-article","created":{"date-parts":[[2018,5,7]],"date-time":"2018-05-07T03:12:21Z","timestamp":1525662741000},"page":"186","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Microbial Diversity and Toxin Risk in Tropical Freshwater Reservoirs of Cape Verde"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9366-9878","authenticated-orcid":false,"given":"Ana P.","family":"Semedo-Aguiar","sequence":"first","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Rua da Quinta Grande n\u00ba6, 2780-156 Oeiras, Portugal"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o Ci\u00eancia para o Desenvolvimento, Rua da Quinta Grande n\u00ba6, 2780-156 Oeiras, Portugal"},{"name":"Universidade Jean Piaget de Cabo Verde, Campus da Praia, Caixa Postal 775, Palmarejo Grande, Praia, Cabo Verde"}]},{"given":"Jose B.","family":"Pereira-Leal","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Rua da Quinta Grande n\u00ba6, 2780-156 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9622-3895","authenticated-orcid":false,"given":"Ricardo B.","family":"Leite","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Rua da Quinta Grande n\u00ba6, 2780-156 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2018,5,5]]},"reference":[{"key":"ref_1","unstructured":"Lobo de Pina, A. 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