{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T02:23:20Z","timestamp":1771295000971,"version":"3.50.1"},"reference-count":26,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,2,29]],"date-time":"2020-02-29T00:00:00Z","timestamp":1582934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Historical reports show that in Portugal, cyanotoxins reports were mainly in the Center (cylindrospermopsins) and South (cylindrospermopsins, saxitoxins) regions of the country apart from the well distributed microcystins. Therefore, in our study, seven freshwater ecosystems located in the North and Center Regions of Portugal were screened between April and September of 2017 for the main cyanotoxins (microcystins, cylindrospermopsins, anatoxin-a, and saxitoxins) by a two methods approach that combined the application of molecular (PCR) and immunological (ELISA) assays. Results from our survey reveal that both methods revealed the presence of all main cyanotoxins. ELISA results showed that 48% of the samples were above (1.6\u201318.8 \u03bcg\/L) the guideline value established for microcystins (1 \u03bcg\/L), while in the remaining cyanotoxins, 33% of the samples were above (1.1\u20136.8 \u03bcg\/L) the guideline value established for anatoxin\u2013a (1 \u03bcg\/L). Further, for saxitoxins, only one sample gave a value above (4.3 \u03bcg\/L) the guideline (3 \u03bcg\/L) and this corresponded to a North Region ecosystem. In the cytotoxin cylindrospermopsins, none of the samples were above the guideline established value (1 \u03bcg\/L). This study will improve the risk assessment strategy in Portugal, as well as advance water quality and water management.<\/jats:p>","DOI":"10.3390\/toxins12030154","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T07:50:53Z","timestamp":1583135453000},"page":"154","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Cyanotoxins Occurrence in Portugal: A New Report on Their Recent Multiplication"],"prefix":"10.3390","volume":"12","author":[{"given":"Cristiana","family":"Moreira","sequence":"first","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos S\/N, 4050-208 Porto, Portugal"}]},{"given":"Cid\u00e1lia","family":"Gomes","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos S\/N, 4050-208 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-2417","authenticated-orcid":false,"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos S\/N, 4050-208 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1328-1732","authenticated-orcid":false,"given":"Agostinho","family":"Antunes","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos S\/N, 4050-208 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/mnfr.200600185","article-title":"Toxins of cyanobacteria","volume":"51","author":"Speijers","year":"2007","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1056\/NEJM199803263381304","article-title":"Liver failure and death after exposure to microcystins at a hemodialyses center in Brazil","volume":"338","author":"Jochimsen","year":"1998","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0041-0101(83)90151-4","article-title":"An outbreak of hepato-enteritis (the Palm Island mystery disease) possibly caused by algal intoxication","volume":"21","author":"Bourke","year":"1983","journal-title":"Toxicon"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1128\/aem.50.5.1292-1295.1985","article-title":"Severe hepatotoxicity caused by the tropical cyanobacterium (blue-green alga) Cylindrospermopsis raciborskii (Woloszynska) Seenaya and Subba Raju isolated from a domestic water supply reservoir","volume":"51","author":"Hawkins","year":"1985","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2729","DOI":"10.3390\/md9122729","article-title":"Cyanotoxins: Bioaccumulation and effects on aquatic animals","volume":"9","year":"2011","journal-title":"Mar. Drugs."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Flores, N.M., Miller, T.R., and Stockwell, J.D. (2018). A Global Analysis of the Relationship between Concentrations of Microcystins in Water and Fish. Front. Mar. Sci., 5.","DOI":"10.3389\/fmars.2018.00030"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1002\/tox.10108","article-title":"Multiplex PCR assay for Cylindrospermopsis raciborskii and cylindrospermopsin-Producing cyanobacteria","volume":"18","author":"Fergusson","year":"2003","journal-title":"Environ. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1007\/s00203-003-0605-9","article-title":"PCR-Based identification of microcystin-Producing genotypes of different cyanobacterial genera","volume":"180","author":"Hisbergues","year":"2003","journal-title":"Arch. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s00239-005-0030-6","article-title":"Functional modeling and phylogenetic distribution of putative cylindrospermopsin biosynthesis enzymes","volume":"62","author":"Kellmann","year":"2006","journal-title":"J. Mol. Evol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.marenvres.2011.10.005","article-title":"Phylogenetic, chemical and morphological diversity of cyanobacteria from Portuguese temperate estuaries","volume":"73","author":"Lopes","year":"2012","journal-title":"Mar. Environ. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1128\/AEM.01988-07","article-title":"Characterization of the gene cluster responsible for cylindrospermopsin biosynthesis","volume":"74","author":"Mihali","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7271","DOI":"10.1128\/AEM.06022-11","article-title":"Anatoxin-A synthetase gene cluster of the cyanobacterium Anabaena sp. strain 37 and molecular methods to detect potential producers","volume":"77","author":"Wang","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.hal.2015.04.005","article-title":"Detection of cyanobacterial sxt genes and paralytic shellfish toxins in freshwater lakes and brackish waters on \u00c5land Islands, Finland","volume":"47","author":"Savela","year":"2015","journal-title":"Harmful Algae"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.1128\/JB.185.9.2774-2785.2003","article-title":"Natural variation in the microcystin synthetase operon mcyABC and impact on microcystin production in Microcystis strains","volume":"185","author":"Mikalsen","year":"2003","journal-title":"J. Bacteriol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1002\/tox.20103","article-title":"Identification of potentially toxic environmental Microcystis by individual and multiple PCR amplification of specific microcystin synthetase gene regions","volume":"20","author":"Ouahid","year":"2005","journal-title":"Environ. Toxicol."},{"key":"ref_16","unstructured":"Ara\u00fajo, F. (2006). Cianobacterias: Ocurrencias y gesti\u00f3n del riesgo en Portugal de 1993 a 2005. Jornada Sobre las Cianobacterias t\u00f3xicas, Problemas Asociados. Seguimiento y Control. Available online: http:\/\/ceh-flumen64.cedex.es\/Ecosistemas\/Jornadas%20Cianos\/F.Araujo.pdf."},{"key":"ref_17","unstructured":"CyanoNews (1996). Toxin Suspect in Mass Killing. Cyanonews, 12, 4. Available online: http:\/\/wwwcyanosite.bio.purdue.edu\/cyanonews\/cn122\/cn122.pdf."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1127\/archiv-hydrobiol\/134\/1995\/295","article-title":"Isolation and characterization of microcystins (heptapeptide hepatotoxins) from portuguese strains of Microcystis aeruginosa Kutz. emend Elekin","volume":"134","author":"Vasconcelos","year":"1995","journal-title":"Arch. f\u00fcr Hydrobiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1016\/S0041-0101(00)00100-8","article-title":"Paralytic shellfish toxins in the freshwater cyanobacterium Aphanizomenon flos-aquae, isolated from Montargil reservoir, Portugal","volume":"38","author":"Pereira","year":"2000","journal-title":"Toxicon"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1007\/s10646-009-0375-5","article-title":"Production of anatoxin-A by cyanobacterial strains isolated from Portuguese fresh water systems","volume":"18","author":"Osswald","year":"2009","journal-title":"Ecotoxicology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.toxicon.2017.02.024","article-title":"First occurrence of cylindrospermopsin in Portugal: A contribution to its continuous global dispersal","volume":"130","author":"Moreira","year":"2017","journal-title":"Toxicon"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1039\/c3em00353a","article-title":"A review on cylindrospermopsin: The global occurrence, detection, toxicity and degradation of a potent cyanotoxin","volume":"15","author":"Hiskia","year":"2013","journal-title":"Environ. Sci. Process. Impacts"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1111\/j.1574-6968.2011.02451.x","article-title":"Preliminary molecular identification of cylindrospermopsin-Producing Cyanobacteria in two Polish lakes (Central Europe)","volume":"326","author":"Jurczak","year":"2012","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s00253-011-3360-x","article-title":"Application of real-Time PCR in the assessment of the toxic cyanobacterium Cylindrospermopsis raciborskii abundance and toxicological potential","volume":"92","author":"Moreira","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Savela, H., Spoof, L., H\u00f6ysniemi, N., Vehni\u00e4inen, M., Mankiewicz-Boczek, J., Jurczak, T., Kokoci\u0144ski, M., and Meriluoto, J. (2017). First report of cyanobacterial paralytic shellfish toxin biosynthesis genes and paralytic shellfish toxin production in Polish freshwater lakes. Adv. Oceanogr. Limnol., 8.","DOI":"10.4081\/aiol.2017.6319"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jhazmat.2016.07.020","article-title":"New SPE-LC-MS\/MS method for simultaneous determination of multi-class cyanobacterial and algal toxins","volume":"5","author":"Zervou","year":"2017","journal-title":"J. Hazard. Mater."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/3\/154\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:02:55Z","timestamp":1760173375000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/3\/154"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,29]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,3]]}},"alternative-id":["toxins12030154"],"URL":"https:\/\/doi.org\/10.3390\/toxins12030154","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,29]]}}}