{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:55:05Z","timestamp":1760237705891,"version":"build-2065373602"},"reference-count":29,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T00:00:00Z","timestamp":1591660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Edible Llayta are cyanobacterial colonies consumed in the Andes highlands. Llayta and four isolated cyanobacteria strains were tested for cyanotoxins (microcystin, nodularin, cylindrospermopsin, saxitoxin and \u03b2-N-methylamino-L-alanine\u2014BMAA) using molecular and chemical methods. All isolates were free of target genes involved in toxin biosynthesis. Only DNA from Llayta amplified the mcyE gene. Presence of microcystin-LR and BMAA in Llayta extracts was discarded by LC\/MS analyses. The analysed Llayta colonies have an incomplete microcystin biosynthetic pathway and are a safe food ingredient.<\/jats:p>","DOI":"10.3390\/toxins12060382","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T06:34:16Z","timestamp":1591684456000},"page":"382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9991-0781","authenticated-orcid":false,"given":"Alexandra","family":"Galetovi\u0107","sequence":"first","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica, Departamento Biom\u00e9dico, Facultad Ciencias de la Salud, and Centre for Biotechnology and Bioengineering, CeBiB, Universidad de Antofagasta, Antofagasta 1270300, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3757-8143","authenticated-orcid":false,"given":"Joana","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental- CIIMAR, 4450-208 Matosinhos, Portugal"}]},{"given":"Raquel","family":"Castelo-Branco","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental- CIIMAR, 4450-208 Matosinhos, Portugal"}]},{"given":"Flavio","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental- CIIMAR, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7611-9943","authenticated-orcid":false,"given":"Benito","family":"G\u00f3mez-Silva","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica, Departamento Biom\u00e9dico, Facultad Ciencias de la Salud, and Centre for Biotechnology and Bioengineering, CeBiB, Universidad de Antofagasta, Antofagasta 1270300, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-2417","authenticated-orcid":false,"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental- CIIMAR, 4450-208 Matosinhos, Portugal"},{"name":"Faculdade de Ci\u00eancias da Universidade do Porto, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1111\/j.1574-6976.2006.00022.x","article-title":"Cyanobacterial peptides\u2014Nature\u2019s own combinatorial biosynthesis","volume":"30","author":"Welker","year":"2006","journal-title":"FEMS Microbiol. 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