{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T08:04:25Z","timestamp":1761897865080,"version":"build-2065373602"},"reference-count":60,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T00:00:00Z","timestamp":1679875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"ValICET","doi-asserted-by":"publisher","award":["PRIMA\/0001\/2020","03\/2022\/BI","UIDB\/00211\/2020"],"award-info":[{"award-number":["PRIMA\/0001\/2020","03\/2022\/BI","UIDB\/00211\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FCT\/MCTES","award":["PRIMA\/0001\/2020","03\/2022\/BI","UIDB\/00211\/2020"],"award-info":[{"award-number":["PRIMA\/0001\/2020","03\/2022\/BI","UIDB\/00211\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Herbal infusions are highly popular beverages consumed daily due to their health benefits and antioxidant properties. However, the presence of plant toxins, such as tropane alkaloids, constitutes a recent health concern for herbal infusions. This work presents an optimized and validated methodology based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) extraction procedure followed by Ultra-High Performance Liquid Chromatography combined with Time-of-Flight Mass Spectrometry (UHPLC-ToF-MS) for the determination of tropane alkaloids (atropine, scopolamine, anisodamine, and homatropine) in herbal infusions, in accordance with criteria established by Commission Recommendation EU No. 2015\/976. One of the seventeen samples was contaminated with atropine, exceeding the current European regulation regarding tropane alkaloids. In addition, this study evaluated the antioxidant capacity of common herbal infusions available on Portuguese markets, indicating the high antioxidant capacity of yerba mate (Ilex paraguariensis), lemon balm (Melissa officinalis), and peppermint (Mentha x piperita).<\/jats:p>","DOI":"10.3390\/toxins15040245","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T06:46:19Z","timestamp":1679899579000},"page":"245","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Bright and Dark Sides of Herbal Infusions: Assessment of Antioxidant Capacity and Determination of Tropane Alkaloids"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4490-5202","authenticated-orcid":false,"given":"Ana Rita Soares","family":"Mateus","sequence":"first","affiliation":[{"name":"National Institute for Agrarian and Veterinary Research (INIAV), I.P., Rua dos Lagidos, Lugar da Madalena, 4485-655 Vila do Conde, Portugal"},{"name":"University of Coimbra, Faculty of Pharmacy, Polo III, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"},{"name":"LAQV, REQUIMTE, Laboratory of Bromatology and Pharmacognosy, Faculty of Pharmacy, University of Coimbra, Polo III, Azinhaga de St\u00aa Comba, 3000-548 Coimbra, Portugal"},{"name":"Center for Study in Animal Science (CECA), ICETA, University of Porto, Apartado, 4050-346 Porto, Portugal"}]},{"given":"Carmen","family":"Crisafulli","sequence":"additional","affiliation":[{"name":"National Institute for Agrarian and Veterinary Research (INIAV), I.P., Rua dos Lagidos, Lugar da Madalena, 4485-655 Vila do Conde, Portugal"}]},{"given":"Matilde","family":"Vilhena","sequence":"additional","affiliation":[{"name":"National Institute for Agrarian and Veterinary Research (INIAV), I.P., Rua dos Lagidos, Lugar da Madalena, 4485-655 Vila do Conde, Portugal"},{"name":"University of Coimbra, Faculty of Pharmacy, Polo III, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"}]},{"given":"S\u00edlvia Cruz","family":"Barros","sequence":"additional","affiliation":[{"name":"National Institute for Agrarian and Veterinary Research (INIAV), I.P., Rua dos Lagidos, Lugar da Madalena, 4485-655 Vila do Conde, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0902-647X","authenticated-orcid":false,"given":"Angelina","family":"Pena","sequence":"additional","affiliation":[{"name":"University of Coimbra, Faculty of Pharmacy, Polo III, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"},{"name":"LAQV, REQUIMTE, Laboratory of Bromatology and Pharmacognosy, Faculty of Pharmacy, University of Coimbra, Polo III, Azinhaga de St\u00aa Comba, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0226-921X","authenticated-orcid":false,"given":"Ana","family":"Sanches Silva","sequence":"additional","affiliation":[{"name":"National Institute for Agrarian and Veterinary Research (INIAV), I.P., Rua dos Lagidos, Lugar da Madalena, 4485-655 Vila do Conde, Portugal"},{"name":"University of Coimbra, Faculty of Pharmacy, Polo III, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"},{"name":"Center for Study in Animal Science (CECA), ICETA, University of Porto, Apartado, 4050-346 Porto, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (Al4AnimalS), 1300-477 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Talib, W.H., AL-Ataby, I.A., Ismail Mahmod, A., Jawarneh, S., al Kury, L.T., and AL-Yasari, I.H. 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