{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:26:10Z","timestamp":1771655170954,"version":"3.50.1"},"reference-count":52,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T00:00:00Z","timestamp":1584662400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Foundation for Science and Technology","award":["SFRH\/BD\/128752\/2017"],"award-info":[{"award-number":["SFRH\/BD\/128752\/2017"]}]},{"name":"FCT\u2014Foundation for Science and Technology","award":["IF\/00376\/2015"],"award-info":[{"award-number":["IF\/00376\/2015"]}]},{"name":"Norte Portugal Regional Operational Programme","award":["NORTE-01-0145-FEDER-000007"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000007"]}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["R01 A1080607"],"award-info":[{"award-number":["R01 A1080607"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Viruses are ubiquitous in nature; however, very few have been identified in the Leporid species. In the fall of 2018, an outbreak of myxomatosis in Iberian hares (Lepus granatensis) was reported in Spain and a novel recombinant myxoma virus strain (MYXV-Tol) was identified. To investigate variability within the recombinant region of the MYXV-Tol and identify any potential viral coinfections, samples (ear, eyelid or vaginal) of Iberian hares were collected from Spain and analyzed. The presence of the recombinant region of the MYXV-Tol was confirmed in six out of eleven samples analyzed. Additionally, a polyomavirus (family Polyomaviridae), representing a putative new species, and anelloviruses (family Anelloviridae) belonging to two putative species were identified, some as coinfection with the recombinant MYXV-Tol. The two polyomavirus genomes were identified in two hares and share &gt;99% genome-wide identity. Based on the analysis of their large T-antigen, the new polyomavirus clusters in a distant clade from other mammals sharing &lt;64% amino acid identity. A total of 14 anelloviruses were identified, which share 63\u201399% genome-wide identity. Overall, our results show a coinfection of different DNA viruses in the studied samples and raise awareness regarding the extensive unsampled diversity of viruses in hares.<\/jats:p>","DOI":"10.3390\/v12030340","type":"journal-article","created":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T11:42:11Z","timestamp":1584704531000},"page":"340","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Coinfections of Novel Polyomavirus, Anelloviruses and a Recombinant Strain of Myxoma Virus-MYXV-Tol Identified in Iberian Hares"],"prefix":"10.3390","volume":"12","author":[{"given":"Ana","family":"\u00c1gueda-Pinto","sequence":"first","affiliation":[{"name":"CIBIO\/InBio\u2014Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Universidade do Porto, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"},{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, 4169-007 Porto, Portugal"},{"name":"Center for Immunotherapy, Vaccines, and Virotherapy (CIVV), The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"}]},{"given":"Simona","family":"Kraberger","sequence":"additional","affiliation":[{"name":"Center for Immunotherapy, Vaccines, and Virotherapy (CIVV), The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"},{"name":"The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine and School of Life sciences, Arizona State University, Tempe, AZ 85287, USA"}]},{"given":"Michael C.","family":"Lund","sequence":"additional","affiliation":[{"name":"The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine and School of Life sciences, Arizona State University, Tempe, AZ 85287, USA"}]},{"given":"Christian","family":"Gort\u00e1zar","sequence":"additional","affiliation":[{"name":"SaBio Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo, 28005 Ciudad Real, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2556-3526","authenticated-orcid":false,"given":"Grant","family":"McFadden","sequence":"additional","affiliation":[{"name":"Center for Immunotherapy, Vaccines, and Virotherapy (CIVV), The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4111-2415","authenticated-orcid":false,"given":"Arvind","family":"Varsani","sequence":"additional","affiliation":[{"name":"The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine and School of Life sciences, Arizona State University, Tempe, AZ 85287, USA"},{"name":"Structural Biology Research Unit, Department of Clinical Laboratory Sciences, University of Cape Town, Cape Town 7701, South Africa"}]},{"given":"Pedro J.","family":"Esteves","sequence":"additional","affiliation":[{"name":"CIBIO\/InBio\u2014Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Universidade do Porto, Campus Agr\u00e1rio de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"},{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, 4169-007 Porto, Portugal"},{"name":"CITS\u2014Centro de Investiga\u00e7\u00e3o em Tecnologias da Sa\u00fade, IPSN, CESPU, 4585-116 Gandra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/1297-9716-44-81","article-title":"Emergence of a new lagovirus related to Rabbit Haemorrhagic Disease Virus","volume":"44","author":"Lavazza","year":"2013","journal-title":"Vet. 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