{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:11:41Z","timestamp":1760235101201,"version":"build-2065373602"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T00:00:00Z","timestamp":1626739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["PTDC\/DTP-SAP\/0859\/2014"],"award-info":[{"award-number":["PTDC\/DTP-SAP\/0859\/2014"]}]},{"name":"Zoology Department of the University of Oxford","award":["Research lectureship"],"award-info":[{"award-number":["Research lectureship"]}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["FAPERJ"],"award-info":[{"award-number":["FAPERJ"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In the last two decades, molecular surveys of arboviruses have enabled the identification of several new viruses, contributing to the knowledge of viral diversity and providing important epidemiological data regarding possible new emerging viruses. A combination of diagnostic assays, Illumina sequencing and phylogenetic inference are here used to characterize two new Massilia phlebovirus strains isolated from sandflies collected in the Arr\u00e1bida region, Portugal. Whole genome sequence analysis enabled their identification as reassortants and the recognition of genomic variants co-circulating in Portugal. Much is still unknown about the life cycle, geographic range, evolutionary forces and public health importance of these viruses in Portugal and elsewhere, and more studies are needed.<\/jats:p>","DOI":"10.3390\/v13071412","type":"journal-article","created":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T11:26:10Z","timestamp":1626780370000},"page":"1412","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Phylogenetic Analysis of Massilia phlebovirus in Portugal"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5445-4142","authenticated-orcid":false,"given":"F\u00e1tima","family":"Amaro","sequence":"first","affiliation":[{"name":"Centre for Vectors and Infectious Diseases Research, National Institute of Health Doutor Ricardo Jorge, Avenida da Liberdade n. 5, 2965-575 \u00c1guas de Moura, Portugal"},{"name":"Instituto de Sa\u00fade Ambiental, Faculdade de Medicina da Universidade de Lisboa, Avenida Prof. Egas Moniz, Ed. Egas Moniz, 1649-028 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7258-1439","authenticated-orcid":false,"given":"L\u00edbia","family":"Z\u00e9-Z\u00e9","sequence":"additional","affiliation":[{"name":"Centre for Vectors and Infectious Diseases Research, National Institute of Health Doutor Ricardo Jorge, Avenida da Liberdade n. 5, 2965-575 \u00c1guas de Moura, Portugal"},{"name":"Campus da FCUL, Biosystems and Integrative Sciences Institute, Edificio TecLabs, Campo Grande, 1749-016 Lisbon, Portugal"}]},{"given":"Jos\u00e9","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Department of Zoology, University of Oxford, Mansfield Road, Oxford OX1 3SZ, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5849-7326","authenticated-orcid":false,"given":"Marta","family":"Giovanetti","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Flaviv\u00edrus, Instituto Oswaldo Cruz Fiocruz, Avenida Brasil, Manguinhos, Rio de Janeiro 21045-900, Brazil"},{"name":"Laborat\u00f3rio de Gen\u00e9tica Celular e Molecular, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos n. 6627, Pampula, Belo Horizonte 31270-901, Brazil"}]},{"given":"Stefanie Christine","family":"Becker","sequence":"additional","affiliation":[{"name":"Centre for Infection Medicine, Institute of Parasitology, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0065-9000","authenticated-orcid":false,"given":"Maria Jo\u00e3o","family":"Alves","sequence":"additional","affiliation":[{"name":"Centre for Vectors and Infectious Diseases Research, National Institute of Health Doutor Ricardo Jorge, Avenida da Liberdade n. 5, 2965-575 \u00c1guas de Moura, Portugal"},{"name":"Instituto de Sa\u00fade Ambiental, Faculdade de Medicina da Universidade de Lisboa, Avenida Prof. Egas Moniz, Ed. Egas Moniz, 1649-028 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1146\/annurev-virology-101416-041718","article-title":"Mechanisms and concepts in RNA virus Population dynamics and evolution","volume":"5","author":"Dolan","year":"2018","journal-title":"Annu. Rev. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/nrmicro.2016.46","article-title":"Reassortment in segmented RNA viruses: Mechanisms and outcomes","volume":"14","author":"McDonald","year":"2016","journal-title":"Nat. Rev. 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