{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:19:32Z","timestamp":1774462772608,"version":"3.50.1"},"reference-count":62,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T00:00:00Z","timestamp":1651708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"individual scientific employment program","doi-asserted-by":"publisher","award":["2020.03171.CEECIND"],"award-info":[{"award-number":["2020.03171.CEECIND"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Resistant bacteria prevail in most chronic skin wounds and other biofilm-related topical skin infections. Bacteriophages (phages) have proven their antimicrobial effectiveness for treating different antibiotic-resistant and multidrug-resistant bacterial infections, but not all phages are effective against biofilms. Phages possessing depolymerases can reach different biofilm layers; however, those that do not have depolymerase activity struggle to penetrate and navigate in the intricate 3D biofilm structure and mainly infect bacteria lodged in the outer biofilm layers. To address this, Pseudomonas aeruginosa phage vB_PaeM-SMS29, a phage with poor antibiofilm properties, was incorporated into polyvinyl alcohol (PVA, Mowiol 4:88) supplemented with 0.1% (v\/v) of glycerol, and cast onto two different microneedle arrays varying in geometry. The dissolving microneedles were thoroughly characterized by microscopy, force-displacement, swelling, phage release and stability. Furthermore, 48 h-old biofilms were formed using the colony biofilm procedure (absence of broth), and the antibiofilm efficacy of the phage-loaded microneedles was evaluated by viable cell counts and microscopy and compared to free phages. The phages in microneedles were fairly stable for six months when stored at 4 \u00b0C, with minor decreases in phage titers observed. The geometry of the microneedles influenced the penetration and force-displacement characteristics but not the antimicrobial efficacy against biofilms. The two PVA microneedles loaded with phages reduced P. aeruginosa PAO1 biofilms by 2.44 to 2.76 log10 CFU\u00b7cm\u22122 at 24 h. These values are significantly higher than the result obtained after the treatment with the free phage (1.09 log10 CFU\u00b7cm\u22122). Overall, this study shows that the distribution of phages caused by the mechanical disruption of biofilms using dissolving microneedles can be an effective delivery method against topical biofilm-related skin infections.<\/jats:p>","DOI":"10.3390\/v14050964","type":"journal-article","created":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T02:46:39Z","timestamp":1651805199000},"page":"964","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Antibiofilm Efficacy of the Pseudomonas aeruginosa\u00a0Pbunavirus vB_PaeM-SMS29 Loaded onto Dissolving Polyvinyl Alcohol Microneedles"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8408-8550","authenticated-orcid":false,"given":"Sanna","family":"Sillankorva","sequence":"first","affiliation":[{"name":"INL\u2014International Iberian Nanotechnology Laboratory, Avenida Mestre Jos\u00e9 Veiga, 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1217-2523","authenticated-orcid":false,"given":"Liliana","family":"Pires","sequence":"additional","affiliation":[{"name":"INL\u2014International Iberian Nanotechnology Laboratory, Avenida Mestre Jos\u00e9 Veiga, 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6637-3462","authenticated-orcid":false,"given":"Lorenzo M.","family":"Pastrana","sequence":"additional","affiliation":[{"name":"INL\u2014International Iberian Nanotechnology Laboratory, Avenida Mestre Jos\u00e9 Veiga, 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4319-2631","authenticated-orcid":false,"given":"Manuel","family":"Ba\u00f1obre-L\u00f3pez","sequence":"additional","affiliation":[{"name":"INL\u2014International Iberian Nanotechnology Laboratory, Avenida Mestre Jos\u00e9 Veiga, 4715-330 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ijantimicag.2009.12.011","article-title":"Antibiotic resistance of bacterial biofilms","volume":"35","author":"Bjarnsholt","year":"2010","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1586\/14787210.2015.1023291","article-title":"Chronic wound infections: The role of Pseudomonas aeruginosa and Staphylococcus aureus","volume":"13","author":"Serra","year":"2015","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Raizman, R., Little, W., and Smith, A.C. (2021). Rapid Diagnosis of Pseudomonas aeruginosa in Wounds with Point-of-Care Fluorescence Imaging. Diagnostics, 11.","DOI":"10.3390\/diagnostics11020280"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1111\/j.1574-695X.2010.00714.x","article-title":"Biofilms in chronic infections\u2014A matter of opportunity\u2014Monospecies biofilms in multispecies infections","volume":"59","author":"Thomsen","year":"2010","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_5","first-page":"fty023","article-title":"Chronic biofilm-based infections: Skewing of the immune response","volume":"76","author":"Hahn","year":"2018","journal-title":"Pathog. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Pinto, A.M., Cerqueira, M.A., Ba\u00f1obre-L\u00f3pes, M., Pastrana, L.M., and Sillankorva, S. (2020). Bacteriophages for chronic wound treatment: From traditional to novel delivery systems. Viruses, 12.","DOI":"10.3390\/v12020235"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"fuab019","DOI":"10.1093\/femsre\/fuab019","article-title":"The clinical path to deliver encapsulated phages and lysins","volume":"45","author":"Pinto","year":"2021","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.biopha.2018.10.078","article-title":"Microneedles: A smart approach and increasing potential for transdermal drug delivery system","volume":"109","author":"Waghule","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"64885","DOI":"10.1039\/C6RA13014C","article-title":"Electrically bistable Ag nanocrystal-embedded metal-organic framework microneedles","volume":"6","author":"Park","year":"2016","journal-title":"RSC Adv."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.msec.2016.03.097","article-title":"A fabrication method of microneedle molds with controlled microstructures","volume":"65","author":"Wang","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"13755","DOI":"10.1073\/pnas.2331316100","article-title":"Microfabricated needles for transdermal delivery of macromolecules and nanoparticles: Fabrication methods and transport studies","volume":"100","author":"McAllister","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1744-7402.2007.02115.x","article-title":"Two Photon Polymerization of Polymer-ceramic Hybrid Materials for Transdermal Drug Delivery","volume":"4","author":"Ovsianikov","year":"2007","journal-title":"Int. J. Appl. Ceram. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.ijpharm.2018.03.031","article-title":"3D printed microneedles for insulin skin delivery","volume":"544","author":"Pere","year":"2018","journal-title":"Int. J. Pharm."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.jconrel.2018.08.042","article-title":"Preparation, properties and challenges of the microneedles-based insulin delivery system","volume":"288","author":"Chen","year":"2018","journal-title":"J. Control. Release"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s13346-015-0238-y","article-title":"Microneedle-based drug and vaccine delivery via nanoporous microneedle arrays","volume":"5","author":"Luttge","year":"2015","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_16","first-page":"945","article-title":"Dissolving and biodegradable microneedle technologies for transdermal sustained delivery of drug and vaccine","volume":"7","author":"Hong","year":"2013","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1021\/acs.nanolett.5b05030","article-title":"Enhanced cancer immunotherapy by microneedle patch-assisted delivery of anti-PD1 antibody","volume":"16","author":"Wang","year":"2016","journal-title":"Nano Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.ejpb.2013.10.001","article-title":"Transdermal delivery of relatively high molecular weight drugs using novel self-dissolving microneedle arrays fabricated from hyaluronic acid and their characteristics and safety after application to the skin","volume":"86","author":"Liu","year":"2014","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"14640","DOI":"10.1021\/acsami.9b02578","article-title":"Microneedle Patch-Mediated Treatment of Bacterial Biofilms","volume":"11","author":"Xu","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.jconrel.2021.10.001","article-title":"Dissolving microneedle patches loaded with amphotericin B microparticles for localised and sustained intradermal delivery: Potential for enhanced treatment of cutaneous fungal infections","volume":"339","author":"Peng","year":"2021","journal-title":"J. Control. Release"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"119643","DOI":"10.1016\/j.ijpharm.2020.119643","article-title":"Microneedle liquid injection system assisted delivery of infection responsive nanoparticles: A promising approach for enhanced site-specific delivery of carvacrol against polymicrobial biofilms-infected wounds","volume":"587","author":"Mir","year":"2020","journal-title":"Int. J. Pharm."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"111786","DOI":"10.1016\/j.msec.2020.111786","article-title":"Selective delivery of silver nanoparticles for improved treatment of biofilm skin infection using bacteria-responsive microparticles loaded into dissolving microneedles","volume":"120","author":"Permana","year":"2021","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5405","DOI":"10.1021\/acsabm.1c00087","article-title":"Flexible microneedle array patch for chronic wound oxygenation and biofilm eradication","volume":"4","author":"Woodhouse","year":"2021","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.mib.2017.09.004","article-title":"Phage therapy as an alternative or complementary strategy to prevent and control biofilm-related infections","volume":"39","author":"Pires","year":"2017","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1177\/2168479017694850","article-title":"Overview of FDA\u2019s Expanded access program for investigational drugs","volume":"51","author":"Jarow","year":"2017","journal-title":"Ther. Innov. Regul. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"World Medical Association (2013). World Medical Association Declaration of Helsinki: Ethical principles for medical research involving human subjects. JAMA-J. Am. Med. Assoc., 310, 2191\u20132194.","DOI":"10.1001\/jama.2013.281053"},{"key":"ref_27","unstructured":"Paris, V., Slawomirski, L., Colbert, A., Delaunay, N., and Oderkirk, J. (2017). Innovation, access and value in pharmaceuticals. New Health Technologies\u2014Managing Access, Value and Sustainability, OECD Publishing."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.3389\/fmicb.2019.02949","article-title":"Diversity and function of phage encoded depolymerases","volume":"10","author":"Knecht","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Melo, L.D.R., Pinto, G., Oliveira, F., Vilas-Boas, D., Almeida, C., Sillankorva, S., Cerca, N., and Azeredo, J. (2020). The protective effect of Staphylococcus epidermidis biofilm matrix against phage predation. Viruses, 12.","DOI":"10.3390\/v12101076"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.ijpharm.2018.12.004","article-title":"Antimicrobial assessment of phage therapy using a porcine model of biofilm infection","volume":"557","author":"Milho","year":"2019","journal-title":"Int. J. Pharm."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Pinto, A.M., Faustino, A., Pastrana, L.M., Ba\u00f1obre-L\u00f3pez, M., and Sillankorva, S. (2021). Pseudomonas aeruginosa PAO 1 in vitro time-kill kinetics using single phages and phage formulations\u2014Modulating Death, Adaptation, and Resistance. Antibiotics, 10.","DOI":"10.3390\/antibiotics10070877"},{"key":"ref_32","unstructured":"Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Protocols. [2nd, ed.]."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Adams, M. (1959). Bacteriophages, Interscience Publishers.","DOI":"10.5962\/bhl.title.6966"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1002\/prot.22499","article-title":"Fast and accurate automatic structure prediction with HHpred","volume":"77","author":"Hildebrand","year":"2009","journal-title":"Proteins Struct. Funct. Bioinform."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"119590","DOI":"10.1016\/j.ijpharm.2020.119590","article-title":"Dissolving microneedles for the delivery of peptides\u2014Towards tolerance-inducing vaccines","volume":"586","author":"Pires","year":"2020","journal-title":"Int. J. Pharm."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3565","DOI":"10.1039\/C4AN01768D","article-title":"Efficient microfluidic negative enrichment of circulating tumor cells in blood using roughened PDMS","volume":"140","author":"Winter","year":"2015","journal-title":"Analyst"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.ijfoodmicro.2018.11.026","article-title":"Bacteriophage \u03d5IBB-PF7A loaded on sodium alginate-based films to prevent microbial meat spoilage","volume":"291","author":"Alves","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.ejpb.2018.05.017","article-title":"Poly (vinyl alcohol) microneedles: Fabrication, characterization, and application for transdermal drug delivery of doxorubicin","volume":"129","author":"Nguyen","year":"2018","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_39","first-page":"1B-1","article-title":"Growing and analyzing static biofilms","volume":"22","author":"Merritt","year":"2005","journal-title":"Curr. Protoc. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"150","DOI":"10.3390\/v5010150","article-title":"Lysogenic conversion and phage resistance development in phage exposed Escherichia coli biofilms","volume":"5","author":"Moons","year":"2013","journal-title":"Viruses"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Guo, Y., Chen, P., Lin, Z., and Wang, T. (2019). Characterization of two Pseudomonas aeruginosa viruses vB_PaeM_SCUT-S1 and vB_PaeM_SCUT-S2. Viruses, 11.","DOI":"10.3390\/v11040318"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"580779","DOI":"10.3389\/fmicb.2020.580779","article-title":"Identification and characterization of new bacteriophages to control multidrug-resistant Pseudomonas aeruginosa biofilm on endotracheal tubes","volume":"11","author":"Oliveira","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.3389\/fmicb.2017.01229","article-title":"A Genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors","volume":"8","author":"Pires","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Men\u00e9ndez, E., Fern\u00e1ndez, L., Guti\u00e9rrez, D., Rodr\u00edguez, A., Mart\u00ednez, B., and Garc\u00eda, P. (2018). Comparative analysis of different preservation techniques for the storage of Staphylococcus phages aimed for the industrial development of phage-based antimicrobial products. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0205728"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Santos, S.B., Carvalho, C.M., Sillankorva, S.R., Nicolau, A., Ferreira, E.C., and Azeredo, J. (2009). The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique. BMC Microbiol., 9.","DOI":"10.1186\/1471-2180-9-148"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"225101","DOI":"10.1088\/0957-4484\/25\/22\/225101","article-title":"Electrospun water-soluble polymer nanofibers for the dehydration and storage of sensitive reagents","volume":"25","author":"Dai","year":"2014","journal-title":"Nanotechnology"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Merabishvili, M., Vervaet, C., Pirnay, J.-P., De Vos, D., Verbeken, G., Mast, J., Chanishvili, N., and Vaneechoutte, M. (2013). Stability of Staphylococcus aureus phage ISP after freeze-drying (lyophilization). PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0068797"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.3892\/etm.2016.3593","article-title":"Effect of glycerol on sustained insulin release from PVA hydrogels and its application in diabetes therapy","volume":"12","author":"Cai","year":"2016","journal-title":"Exp. Ther. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2312","DOI":"10.1002\/bit.27743","article-title":"Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications","volume":"118","author":"Pushp","year":"2021","journal-title":"Biotechnol. Bioeng."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Demir, Y.K., Akan, Z., and Kerimoglu, O. (2013). Characterization of Polymeric Microneedle Arrays for Transdermal Drug Delivery. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0077289"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s40486-020-00113-0","article-title":"Comparison of polymers to enhance mechanical properties of microneedles for bio-medical applications","volume":"8","author":"Bonfante","year":"2020","journal-title":"Micro Nano Syst. Lett."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"54","DOI":"10.3389\/fbioe.2018.00054","article-title":"Fabrication of circular obelisk-type multilayer microneedles using micro-milling and spray deposition","volume":"6","author":"Kim","year":"2018","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1080\/01694243.2012.705101","article-title":"The effects of geometry on skin penetration and failure of polymer microneedles","volume":"27","author":"Gittard","year":"2013","journal-title":"J. Adhes. Sci. Technol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ijpharm.2014.05.042","article-title":"A proposed model membrane and test method for microneedle insertion studies","volume":"472","author":"Moore","year":"2014","journal-title":"Int. J. Pharm."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1007\/s11095-020-02887-9","article-title":"Two-photon polymerisation 3D printing of microneedle array templates with versatile designs: Application in the development of polymeric drug delivery systems","volume":"37","author":"Cordeiro","year":"2020","journal-title":"Pharm. Res."},{"key":"ref_56","first-page":"35","article-title":"Long term bacteriophage preservation","volume":"38","author":"Ackermann","year":"2004","journal-title":"WFCC Newsl."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S1473-3099(18)30482-1","article-title":"Efficacy and tolerability of a cocktail of bacteriophages to treat burn wounds infected by Pseudomonas aeruginosa (PhagoBurn): A randomised, controlled, double-blind phase 1\/2 trial","volume":"19","author":"Jault","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1016\/j.nano.2017.08.008","article-title":"Immobilization of bacteriophage in wound-dressing nanostructure","volume":"13","author":"Nogueira","year":"2017","journal-title":"Nanomed. Nanotechnol. Biol. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1038\/s41598-018-38318-4","article-title":"Fibrin glue as a local drug-delivery system for bacteriophage PA5","volume":"9","author":"Rubalskii","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1002\/jbm.a.36790","article-title":"Bacteriophage delivering hydrogels reduce biofilm formation in vitro and infection in vivo","volume":"108","author":"Wroe","year":"2020","journal-title":"J. Biomed. Mater. Res.-Part A"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.ijpharm.2018.04.063","article-title":"Microfluidic-assisted bacteriophage encapsulation into liposomes","volume":"545","author":"Leung","year":"2018","journal-title":"Int. J. Pharm."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"120850","DOI":"10.1016\/j.ijpharm.2021.120850","article-title":"Hydrogel formulations containing non-ionic polymers for topical delivery of bacteriophages","volume":"605","author":"Chang","year":"2021","journal-title":"Int. J. Pharm."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/5\/964\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:06:15Z","timestamp":1760137575000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/5\/964"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,5]]},"references-count":62,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["v14050964"],"URL":"https:\/\/doi.org\/10.3390\/v14050964","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,5]]}}}