{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T15:44:42Z","timestamp":1767973482297,"version":"3.49.0"},"reference-count":97,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,3]],"date-time":"2024-02-03T00:00:00Z","timestamp":1706918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Human type A rotavirus (RV-A) is world-recognized as the major pathogen causing viral gastroenteritis in children under 5 years of age. The literature indicates a substantial increase in the diversity of rotavirus strains across continents, especially in Africa, which can pose significant challenges including an increase of disease burden and a reduction of vaccines\u2019 effectiveness. However, few studies have mapped the variety of circulating virus strains in different regions, which may hamper decisions on epidemiological surveillance and preventive public health measures. Thus, our aim was to compile the most updated available evidence on the genetic profile of RV-A among children in Africa and determine the prevalence of different genotypes according to the geographical regions by means of a broad systematic review. Systematic searches were performed in PubMed, Scopus, Web of Science, and Scielo without language, time limits, or geographical restrictions within the African continent. We selected full-text peer-reviewed articles assessing the genetic profile (i.e., genotyping) of RV-A in children up to 5 years old in Africa. Overall, 682 records were retrieved, resulting in 75 studies included for evidence synthesis. These studies were published between 1999 and 2022, were conducted in 28 countries from the five African regions, and 48% of the studies were carried out for 24 months or more. Most studies (n = 55; 73.3%) evaluated RV-A cases before the introduction of the vaccines, while around 20% of studies (n = 13) presented data after the vaccine approval in each country. Only seven (9.3%) studies compared evidence from both periods (pre- and post-vaccine introduction). Genotyping methods to assess RV-A varied between RT-PCR, nested or multiplex RT-PCR, testing only the most common P and G-types. We observed G1 and P[8] to be the most prevalent strains in Africa, with values around 31% and 43%, respectively. Yet if all the genotypes with the following highest prevalence were added ((G1 + G2, G3, G9) and (P[8] + P[6], P[4])), these figures would represent 80% and 99% of the total prevalence. The combination G1P[8] was the most reported in the studies (around 22%). This review study demonstrated an increased strain diversity in the past two decades, which could represent a challenge to the efficacy of the current vaccine.<\/jats:p>","DOI":"10.3390\/v16020243","type":"journal-article","created":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T05:22:44Z","timestamp":1707110564000},"page":"243","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genetic Profile of Rotavirus Type A in Children under 5 Years Old in Africa: A Systematic Review of Prevalence"],"prefix":"10.3390","volume":"16","author":[{"given":"Sandra","family":"Miranda","sequence":"first","affiliation":[{"name":"Faculdade de Medicine, Universidade Agostinho Neto, Luanda, Angola"},{"name":"CISA-Centro de Investiga\u00e7\u00e3o em Sa\u00fade de Angola, Caxito, Bengo, Angola"},{"name":"Cl\u00ednica Girassol, Luanda, Angola"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-8608","authenticated-orcid":false,"given":"Fernanda S.","family":"Tonin","sequence":"additional","affiliation":[{"name":"ESTeSL-Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisboa, Portugal"},{"name":"Pharmaceutical Sciences Postgraduate Program, Federal University of Paran\u00e1, Curitiba 80210-170, Brazil"}]},{"given":"Carlos","family":"Pinto-Sousa","sequence":"additional","affiliation":[{"name":"Faculdade de Medicine, Universidade Agostinho Neto, Luanda, Angola"},{"name":"UPRA-Universidade Privada de Angola, Luanda, Angola"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9378-3643","authenticated-orcid":false,"given":"Elsa","family":"Fortes-Gabriel","sequence":"additional","affiliation":[{"name":"CISA-Centro de Investiga\u00e7\u00e3o em Sa\u00fade de Angola, Caxito, Bengo, Angola"},{"name":"ISTM- Instituto Superior T\u00e9cnico Militar, Luanda, Angola"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6394-658X","authenticated-orcid":false,"given":"Miguel","family":"Brito","sequence":"additional","affiliation":[{"name":"CISA-Centro de Investiga\u00e7\u00e3o em Sa\u00fade de Angola, Caxito, Bengo, Angola"},{"name":"ESTeSL-Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,3]]},"reference":[{"key":"ref_1","unstructured":"Bernadeta, D., Hannah, R., and Max, R. (2021, May 14). Diarrheal Diseases\u2014Our World in Data November 2019. Available online: https:\/\/ourworldindata.org\/diarrheal-diseases#burden-of-diarrheal-diseases."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/S2352-4642(21)00311-4","article-title":"Global, regional, and national causes of under-5 mortality in 2000\u201319: An updated systematic analysis with implications for the Sustainable Development Goals","volume":"6","author":"Perin","year":"2022","journal-title":"Lancet Child Adolesc. Health"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Damtie, D., Melku, M., Tessema, B., and Vlasova, A.N. (2020). Prevalence and Genetic Diversity of Rotaviruses among under-Five Children in Ethiopia: A Systematic Review and Meta-Analysis. Viruses, 12.","DOI":"10.3390\/v12010062"},{"key":"ref_4","unstructured":"(2022, December 01). Manual for the Surveillance of Vaccine-Preventable Diseases|CDC, Available online: https:\/\/www.cdc.gov\/vaccines\/pubs\/surv-manual\/index.html."},{"key":"ref_5","unstructured":"World Health Organization (2021). Rotavirus vaccines: WHO position paper\u2014July 2021\u2014Vaccines antirotavirus: Note de synth\u00e8se de l\u2019OMS\u2014Juillet 2021. Wkly Epidemiol. Rec., 96, 219\u2013301."},{"key":"ref_6","unstructured":"GBD 2019 Diseases and Injuries Collaborators (2020). Global burden of 369 diseases and injuries in 204 countries and territories, 1990\u20132019: A systematic analysis for the Global Burden of Disease Study 2019. Lancet, 396, 1204\u20131222."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Godfrey, O., Zhang, W., Amponsem-Boateng, C., Oppong, T.B., Zhao, Q.L., and Li, D. (2020). Evidence of rotavirus vaccine impact in sub-Saharan Africa: Systematic review and meta-analysis. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0232113"},{"key":"ref_8","unstructured":"(2021, January 16). Technical Resources|Rota Council. Available online: https:\/\/preventrotavirus.org\/resources\/technical-resources\/."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Gasparinho, C., Piedade, J., Mirante, M.C., Mendes, C., Mayer, C., Nery, S.V., Brito, M., and Istrate, C. (2017). Characterization of rotavirus infection in children with acute gastroenteritis in Bengo province, Northwestern Angola, prior to vaccine introduction. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0176046"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"589","DOI":"10.3201\/eid1804.110973","article-title":"Emergence of Unusual G6P[6] Rotaviruses in Children, Burkina Faso, 2009\u20132010","volume":"18","author":"Nordgren","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"S34","DOI":"10.1086\/653555","article-title":"Rotavirus Strain Types Circulating in Africa: Review of Studies Published during 1997\u20132006","volume":"202","author":"Todd","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s00705-017-3575-y","article-title":"Rotavirus A strains obtained from children with acute gastroenteritis in Mozambique, 2012-2013: G and P genotypes and phylogenetic analysis of VP7 and partial VP4 genes","volume":"163","author":"Strydom","year":"2018","journal-title":"Arch. Virol."},{"key":"ref_13","first-page":"525","article-title":"Epidemiology of rotavirus diarrhoea in Africa: A review to assess the need for rotavirus immunization","volume":"76","author":"Cunliffe","year":"1998","journal-title":"Bull. World Health Organ."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3923\/pjbs.2017.59.69","article-title":"Molecular Epidemiology of Rotavirus in Children under Five in Africa (2006\u20132016): A Systematic Review","volume":"20","author":"Ouermi","year":"2017","journal-title":"Pak. J. Biol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1002\/jmv.24510","article-title":"Molecular epidemiology of rotavirus in four provinces of Angola before vaccine introduction","volume":"88","author":"Esteves","year":"2016","journal-title":"J. Med. Virol."},{"key":"ref_16","unstructured":"Aromataris, E., and Munn, Z. (2022, December 02). JBI Manual for Evidence Synthesis. Available online: https:\/\/jbi-global-wiki.refined.site\/space\/MANUAL."},{"key":"ref_17","unstructured":"Higgins, J.P.T., Thomas, J., Chandler, J., Cumpston, M., Li, T., Page, M.J., and Welch, V.A. (2022, December 02). Cochrane Handbook for Systematic Reviews of Interventions. Available online: https:\/\/jhu.pure.elsevier.com\/en\/publications\/cochrane-handbook-for-systematic-reviews-of-interventions."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 statement: An updated guideline for reporting systematic reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1093\/oxfordjournals.aje.a010080","article-title":"Epidemiology of rotavirus diarrhea in Egyptian children and implications for disease control","volume":"150","author":"Naficy","year":"1999","journal-title":"Am. J. Epidemiol."},{"key":"ref_20","first-page":"e60288","article-title":"Detection and Genotyping of Viral Gastroenteritis in Hospitalized Children Below Five Years Old in Cairo, Egypt","volume":"6","author":"Allayeh","year":"2018","journal-title":"Arch. Pediatr. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"433","DOI":"10.5604\/17331331.1227669","article-title":"Genotyping and Clinicoepidemiological Characterization of Rotavirus Acute Gastroenteritis in Egyptian Children","volume":"65","author":"Saudy","year":"2017","journal-title":"Polish J. Microbiol."},{"key":"ref_22","first-page":"512","article-title":"Genotyping of rota virus causing gastroenteritis in Egyptian children","volume":"7","author":"Elnady","year":"2016","journal-title":"Res. J. Pharm. Biol. Chem. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"S263","DOI":"10.1086\/653581","article-title":"Rotavirus Genotypes among Hospitalized Children in Egypt, 2000\u20132002","volume":"202","author":"Matson","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"S62","DOI":"10.1097\/INF.0000000000000052","article-title":"Rotavirus Genotypes Associated with Acute Diarrhea in Egyptian Infants","volume":"33","author":"Ahmed","year":"2014","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"S70","DOI":"10.1086\/605048","article-title":"Epidemiology of Rotavirus Gastroenteritis among Children <5 Years of Age in Morocco during 1 Year of Sentinel Hospital Surveillance, June 2006\u2013May 2007","volume":"200","author":"Benhafid","year":"2009","journal-title":"J. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1002\/jmv.23445","article-title":"Diversity of rotavirus strains circulating in children under 5 years of age admitted to hospital for acute gastroenteritis in Morocco, June 2006 to May 2009","volume":"85","author":"Benhafid","year":"2013","journal-title":"J. Med. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"El Qazoui, M., Oumzil, H., Baassi, L., El Omari, N., Sadki, K., Amzazi, S., Benhafid, M., and El Aouad, R. (2014). Rotavirus and norovirus infections among acute gastroenteritis children in Morocco. BMC Infect. Dis., 14.","DOI":"10.1186\/1471-2334-14-300"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e79","DOI":"10.1016\/j.patbio.2009.05.007","article-title":"\u00c9volution des souches de Rotavirus du groupe A en circulation en Tunisie sur une p\u00e9riode de trois ans (2005\u20132007)","volume":"59","author":"Chouikha","year":"2011","journal-title":"Pathol. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"S252","DOI":"10.1086\/653580","article-title":"Rotavirus Strain Diversity in the Centre Coast of Tunisia from 2000 through 2003","volume":"202","author":"Trabelsi","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.respe.2012.04.005","article-title":"\u00c9pid\u00e9miologie des gastroent\u00e9rites \u00e0 rotavirus chez les enfants \u00e2g\u00e9s de moins de cinq ans en Tunisie\u2014R\u00e9sultats de la surveillance sentinelle hospitali\u00e8re 2009 \u00e0 2011","volume":"60","author":"Soltani","year":"2012","journal-title":"Rev. Epidemiol. Sante Publique"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1002\/jmv.20919","article-title":"Group A rotavirus strains circulating in the eastern center of Tunisia during a ten-year period (1995\u20132004)","volume":"79","author":"Chouikha","year":"2007","journal-title":"J. Med. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1099\/jmm.0.000305","article-title":"Distribution of rotavirus VP7 and VP4 genotypes circulating in Tunisia from 2009 to 2014: Emergence of the genotype G12","volume":"65","author":"Moussa","year":"2016","journal-title":"J. Med. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1099\/jmm.0.001031","article-title":"Molecular characterization of group A rotavirus among children aged under 5 years in Tunisia, 2015\u20132017","volume":"68","author":"Bennour","year":"2019","journal-title":"J. Med. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Agbla, J.M., Esona, M.D., Agbankpe, A.J., Capo-Chichi, A., Gautam, R., Dougnon, T.V., Razack, O., Bowen, M.D., and Bankole, H.S. (2020). Molecular characteristics of rotavirus genotypes circulating in the south of Benin, 2016\u20132018. BMC Res. Notes, 13.","DOI":"10.1186\/s13104-020-05332-7"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"S225","DOI":"10.1086\/653574","article-title":"Antigenic and Molecular Characterization of Unusual Rotavirus Strains in Burkina Faso in 1999","volume":"202","author":"Steele","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1002\/jmv.22137","article-title":"Genotype diversity of group A rotavirus strains in children with acute diarrhea in urban Burkina Faso, 2008\u20132010","volume":"83","author":"Bonkoungou","year":"2011","journal-title":"J. Med. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e245","DOI":"10.1017\/S0950268820002320","article-title":"Norovirus and rotavirus in children hospitalised with diarrhoea after rotavirus vaccine introduction in Burkina Faso","volume":"148","author":"Bonkoungou","year":"2020","journal-title":"Epidemiol. Infect."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1016\/j.meegid.2012.08.014","article-title":"Rotavirus in diarrheal children in rural Burkina Faso: High prevalence of genotype G6P[6]","volume":"12","author":"Nordgren","year":"2012","journal-title":"Infect. Genet. Evol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1002\/jmv.25213","article-title":"Rotavirus and norovirus in children with severe diarrhea in Burkina Faso before rotavirus vaccine introduction","volume":"90","author":"Bonkoungou","year":"2018","journal-title":"J. Med. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1128\/JCM.39.5.1981-1984.2001","article-title":"Rotavirus G and P Genotypes in Rural Ghana","volume":"39","author":"Asmah","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1002\/1096-9071(200101)63:1<67::AID-JMV1010>3.0.CO;2-T","article-title":"Prevalence of unusual human rotavirus strains in Ghanaian children","volume":"63","author":"Armah","year":"2001","journal-title":"J. Med. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1046\/j.1365-3156.2003.01097.x","article-title":"Incidence and risk factors of paediatric rotavirus diarrhoea in northern Ghana","volume":"8","author":"Binka","year":"2003","journal-title":"Trop. Med. Int. Health"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1186\/1743-422X-10-287","article-title":"E Rotavirus genotypes associated with childhood severe acute diarrhoea in southern Ghana: A cross-sectional study","volume":"10","author":"Sagoe","year":"2013","journal-title":"Virol. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"7238","DOI":"10.1016\/j.vaccine.2018.01.010","article-title":"Rotavirus strain distribution in Ghana pre- and post- rotavirus vaccine introduction","volume":"36","author":"Lartey","year":"2018","journal-title":"Vaccine"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1002\/jmv.25542","article-title":"Distribution of rotavirus genotypes in the postvaccine introduction era in Ashaiman, Greater Accra Region, Ghana, 2014\u20132016","volume":"91","author":"Letsa","year":"2019","journal-title":"J. Med. Virol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"331","DOI":"10.7883\/yoken.JJID.2014.407","article-title":"Rotavirus Infection in Children with Diarrhea at Korle-Bu Teaching Hospital, Ghana","volume":"69","author":"Damanka","year":"2016","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jcv.2004.12.017","article-title":"Characterisation of rotavirus strains among hospi-talised and non-hospitalised children in Guinea-Bissau, 2002: A high frequency of mixed infections with serotype G8","volume":"34","author":"Nielsen","year":"2005","journal-title":"J. Clin. Virol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/s12985-018-0973-z","article-title":"Genotypic characterization of rotavirus in children under 5 years circulating in C\u00f4te D\u2019Ivoire from 2010 to 2013","volume":"15","author":"Meite","year":"2018","journal-title":"Virol. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"573","DOI":"10.3201\/eid2004.131328","article-title":"Rotavirus Surveillance in Urban and Rural Areas of Niger, April 2010\u2013March 2012","volume":"20","author":"Page","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_50","first-page":"59","article-title":"Diversity of human rotavirus VP6, VP7, and VP4 in Lagos State, Nigeria","volume":"20","author":"Audu","year":"2002","journal-title":"J. Health Popul. Nutr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1007\/s00705-015-2389-z","article-title":"Molecular characterization of group A rotavirus strains detected in children with diarrhea admitted to Nigerian hospitals in 2013","volume":"160","author":"Ianiro","year":"2015","journal-title":"Arch. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S2222-1808(16)61080-0","article-title":"Genetic diversity of rotavirus strains in children with diarrhea in Lagos, Nigeria","volume":"6","author":"Ayolabi","year":"2016","journal-title":"Asian Pac. J. Trop. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4103\/1117-1936.180108","article-title":"Rota virus genotypes and the clinical severity of Diarrhoea among children under 5 years of age","volume":"23","author":"Uzoma","year":"2016","journal-title":"Niger. Postgrad. Med. J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/jmv.24949","article-title":"Group A rotaviruses circulating prior to a national immunization programme in Nigeria: Clinical manifestations, high G12P[8] frequency, intra-genotypic divergence of VP4 and VP7","volume":"90","author":"Japhet","year":"2017","journal-title":"J. Med. Virol."},{"key":"ref_55","first-page":"187","article-title":"Molecular characterization of rotavirus genotype-A in children with acute diarrhea attending a tertiary hospital in Ilorin, Nigeria","volume":"8","author":"Amadu","year":"2019","journal-title":"Int. J. Health Allied Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1002\/jmv.21999","article-title":"A first report on the characterization of rotavirus strains in Sierra Leone","volume":"83","author":"Jere","year":"2011","journal-title":"J. Med. Virol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"S64","DOI":"10.1086\/653571","article-title":"Diversity of Rotavirus Strains Circulating in West Africa from 1996 to 2000","volume":"202","author":"Armah","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"S205","DOI":"10.1086\/653575","article-title":"Rotavirus VP4 and VP7 Genotypes Circulating in Cameroon: Identification of Unusual Types","volume":"202","author":"Esona","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.meegid.2014.08.019","article-title":"Molecular surveillance of rotavirus strains circulating in Yaound\u00e9, Cameroon, September 2007\u2013December 2012","volume":"28","author":"Boula","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1002\/jmv.23603","article-title":"One year survey of human rotavirus strains suggests the emergence of genotype G12 in Cameroon","volume":"85","author":"Ndze","year":"2013","journal-title":"J. Med. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.meegid.2014.08.023","article-title":"Molecular surveillance of rotavirus infection in Bangui, Central African Republic, October 2011\u2013September 2013","volume":"28","author":"Gody","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Moure, U.A.E., Banga-Mingo, V., Gody, J.C., Mwenda, J.M., Fandema, J., Waku-Kouomou, D., Manengu, C., Koyazegbe, T.D., Esona, M.D., and Bowen, M.D. (2018). Emergence of G12 and G9 rotavirus genotypes in the Central African Republic, January 2014 to February 2016. BMC Res. Notes, 11.","DOI":"10.1186\/s13104-017-3122-7"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1002\/jmv.24382","article-title":"Molecular epidemiology and surveillance of circulating rotavirus and adenovirus in Congolese children with gastroenteritis","volume":"88","author":"Mayindou","year":"2016","journal-title":"J. Med. Virol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"S193","DOI":"10.1086\/653576","article-title":"Characterization of Human Rotavirus Recovered from Children with Acute Diarrhea in Kinshasa, Democratic Republic of Congo","volume":"202","author":"Kabue","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1097\/INF.0000000000000212","article-title":"Molecular Surveillance of Rotavirus Infection in the Democratic Republic of the Congo August 2009 to June 2012","volume":"33","author":"Pukuta","year":"2014","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s00705-014-2244-7","article-title":"High rate of detection of G8P[6] rotavirus in children with acute gastroenteritis in S\u00e3o Tom\u00e9 and Pr\u00edncipe","volume":"160","author":"Istrate","year":"2015","journal-title":"Arch. Virol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"S28","DOI":"10.1097\/INF.0000000000000048","article-title":"Hospital-based Surveillance for Rotavirus Gastroenteritis in Children Younger Than 5 Years of Age in Ethiopia","volume":"33","author":"Abebe","year":"2014","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.meegid.2018.08.016","article-title":"Molecular epidemiology of rotaviruses in Northwest Ethiopia after national vaccine introduction","volume":"65","author":"Gelaw","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"S187","DOI":"10.1086\/653564","article-title":"Characterization of Human Rotavirus Strains from Children with Diarrhea in Nairobi and Kisumu, Kenya, between 2000 and 2002","volume":"202","author":"Nyangao","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1111\/tmi.13040","article-title":"Impact of rotavirus vaccination on rotavirus hospitalisation rates among a resource-limited rural population in Mbita, Western Kenya","volume":"23","author":"Wandera","year":"2018","journal-title":"Trop. Med. Int. Health"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"39","DOI":"10.4314\/ejhs.v25i1.6","article-title":"Human Rotavirus Group a Serotypes Causing Gastroenteritis in Children Less Than 5 Years and HIV-Infected Adults in Viwandani Slum, Nairobi","volume":"25","author":"Raini","year":"2015","journal-title":"Ethiop. J. Health Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"S85","DOI":"10.1097\/INF.0000000000000059","article-title":"Rotavirus G and P types circulating in the eastern region of Kenya: Predominance of G9 and emergence of G12 genotypes","volume":"33","author":"Kiulia","year":"2014","journal-title":"Pediatr. Infect Dis. J."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1002\/jmv.24691","article-title":"Molecular epidemiology of rotavirus gastroenteritis in Central Kenya before vaccine introduction, 2009-2014","volume":"89","author":"Wandera","year":"2016","journal-title":"J. Med. Virol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1002\/(SICI)1096-9071(199903)57:3<308::AID-JMV15>3.0.CO;2-B","article-title":"Rotavirus G and P types in children with acute diarrhea in Blantyre, Malawi, from 1997 to 1998: Predominance of novel P[6]G8 strains","volume":"57","author":"Cunliffe","year":"1999","journal-title":"J. Med. Virol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1128\/JCM.39.3.836-843.2001","article-title":"Rotavirus Strain Diversity in Blantyre, Malawi, from 1997 to 1999","volume":"39","author":"Cunliffe","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"S168","DOI":"10.1086\/653577","article-title":"Epidemiology of Rotavirus Infection in Children in Blantyre, Malawi, 1997\u20132007","volume":"202","author":"Cunliffe","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_77","first-page":"42","article-title":"Surveillance of rotavirus gastro-enteritis in children in Blantyre, Malawi","volume":"33","author":"Turner","year":"2013","journal-title":"Ann. Trop. Paediatr."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Jo\u00e3o, E.D., Munlela, B., Chissaque, A., Chila\u00fale, J., Langa, J., Augusto, O., Boene, S.S., Anapakala, E., Sambo, J., and Guimar\u00e3es, E. (2020). Molecular Epidemiology of Rotavirus A Strains Pre- and Post-Vaccine (Rotarix\u00ae) Introduction in Mozambique, 2012\u20132019: Emergence of Genotypes G3P[4] and G3P[8]. Pathogens, 9.","DOI":"10.3390\/pathogens9090671"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Chissaque, A., Bauhofer, A.F.L., Cossa-Moiane, I., Sitoe, E., Munlela, B., Jo\u00e3o, E.D., Langa, J.S., Chila\u00fale, J.J., Boene, S.S., and Cassocera, M. (2021). Rotavirus A infection in pre- and post-vaccine period: Risk factors, genotypes distribution by vaccination status and age of children in Nampula Province, Northern Mozambique (2015\u20132019). PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0255720"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Manjate, F., Jo\u00e3o, E.D., Chirinda, P., Garrine, M., Vubil, D., Nobela, N., Kotloff, K., Nataro, J.P., Nhampossa, T., and Ac\u00e1cio, S. (2022). Molecular Epidemiology of Rotavirus Strains in Symptomatic and Asymptomatic Children in Manhi\u00e7a District, Southern Mozambique 2008\u20132019. Viruses, 14.","DOI":"10.3390\/v14010134"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1093\/tropej\/fmu028","article-title":"Predominance of Rotavirus G1[P8] Genotype among Under-Five Children with Gastroenteritis in Mwanza, Tanzania","volume":"60","author":"Hokororo","year":"2014","journal-title":"J. Trop. Pediatr."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"S41","DOI":"10.1097\/INF.0000000000000070","article-title":"Rotavirus Prevalence and Genotypes Among Children Younger Than 5 Years With Acute Diarrhea at Mulago National Referral Hospital, Kampala, Uganda","volume":"33","author":"Odiit","year":"2014","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1007\/s00705-015-2742-2","article-title":"The epidemiology of rotavirus disease in under-five-year-old children hospitalized with acute diarrhea in central Uganda, 2012\u20132013","volume":"161","author":"Bwogi","year":"2016","journal-title":"Arch. Virol."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Simwaka, J., Seheri, M., Mulundu, G., Kaonga, P., Mwenda, J.M., Chilengi, R., Mpabalwani, E., and Munsaka, S. (2021). Rotavirus breakthrough infections responsible for gastroenteritis in vaccinated infants who presented with acute diarrhoea at University Teaching Hospitals, Children\u2019s Hospital in 2016, in Lusaka Zambia. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0246025"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"7248","DOI":"10.1016\/j.vaccine.2018.03.069","article-title":"Distribution of rotavirus genotypes associated with acute diarrhoea in Zimbabwean children less than five years old before and after rotavirus vaccine introduction","volume":"36","author":"Mukaratirwa","year":"2018","journal-title":"Vaccine"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"S45","DOI":"10.1097\/INF.0000000000000050","article-title":"Epidemiologic and genotypic characteristics of rotavirus strains detected in children less than 5 years of age with gastroenteritis treated at 3 pediatric hospitals in Zimbabwe during 2008\u20132011","volume":"33","author":"Mukaratirwa","year":"2014","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"6324","DOI":"10.1016\/j.vaccine.2019.09.022","article-title":"Diversity of Rotavirus Strains Circulating in Botswana before and after introduction of the Monovalent Rotavirus Vaccine","volume":"37","author":"Mokomane","year":"2019","journal-title":"Vaccine"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"S162","DOI":"10.1086\/653562","article-title":"Characterization of Rotavirus Strains Detected in Windhoek, Namibia during 1998\u20131999","volume":"202","author":"Page","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"S139","DOI":"10.1086\/653559","article-title":"Characterization and Molecular Epidemiology of Rotavirus Strains Recovered in Northern Pretoria, South Africa during 2003\u20132006","volume":"202","author":"Seheri","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/S1473-3099(09)70179-3","article-title":"Burden of disease and circulating serotypes of rotavirus infection in sub-Saharan Africa: Systematic review and meta-analysis","volume":"9","author":"Grais","year":"2009","journal-title":"Lancet Infect. Dis."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"S76","DOI":"10.1097\/INF.0000000000000053","article-title":"Update of Rotavirus Strains Circulating in Africa From 2007 Through 2011","volume":"33","author":"Seheri","year":"2014","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1002\/rmv.448","article-title":"Global distribution of rotavirus serotypes\/genotypes and its implication for the development and implementation of an effective rotavirus vaccine","volume":"15","author":"Santos","year":"2004","journal-title":"Rev. Med. Virol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.jcv.2004.04.009","article-title":"Rotavirus genotyping: Keeping up with an evolving population of human rotaviruses","volume":"31","author":"Kang","year":"2004","journal-title":"J. Clin. Virol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1128\/CMR.00029-07","article-title":"Rotavirus Vaccines: An Overview","volume":"21","author":"Dennehy","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1093\/infdis\/jix186","article-title":"Global impact of rotavirus vaccination on childhood hospitalizations and mortality from diarrhea","volume":"215","author":"Burnett","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1093\/cid\/ciad017","article-title":"The Full Impact of Rotavirus Vaccines in Africa Has Yet to Be Realized","volume":"76","author":"Steele","year":"2023","journal-title":"Clin. Infect. Dis."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Hoxie, I., and Dennehy, J.J. (2021). Rotavirus A Genome Segments Show Distinct Segregation and Codon Usage Patterns. 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