{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:52:57Z","timestamp":1760143977486,"version":"build-2065373602"},"reference-count":68,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T00:00:00Z","timestamp":1710201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT)","doi-asserted-by":"publisher","award":["2021.09380.BD","UIDB\/04750\/2020","LA\/P\/0064\/2020","UIDP\/00772\/2020","LA\/P\/0059\/2020"],"award-info":[{"award-number":["2021.09380.BD","UIDB\/04750\/2020","LA\/P\/0064\/2020","UIDP\/00772\/2020","LA\/P\/0059\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT","doi-asserted-by":"publisher","award":["2021.09380.BD","UIDB\/04750\/2020","LA\/P\/0064\/2020","UIDP\/00772\/2020","LA\/P\/0059\/2020"],"award-info":[{"award-number":["2021.09380.BD","UIDB\/04750\/2020","LA\/P\/0064\/2020","UIDP\/00772\/2020","LA\/P\/0059\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Coronaviruses (CoVs) are RNA viruses capable of infecting a wide range of hosts, including mammals and birds, and have caused significant epidemics such as the ongoing COVID-19 pandemic. Bats, the second most diverse mammalian order, are hosts for various CoVs due to their unique immune responses and ecological traits. This study investigates CoV prevalence in crevice- and tree-dwelling bats in Portugal, a country with limited prior research on bat CoVs. Using nested RT-PCR and sequencing, we screened 87 stool samples from bats, identifying one sample (1.15%) that was positive for Alphacoronavirus, belonging to Pipistrellus pipistrellus. Phylogenetic analysis revealed close genetic relationships with Alphacoronavirus strains from the same bat species in Europe. The low prevalence suggests habitat-specific differences in viral transmission, with cave-dwelling bats exhibiting higher CoV prevalence due to population density and behaviour. These findings underscore the necessity for sustained surveillance efforts aimed at comprehending CoV dynamics within bat populations, especially concerning the risk of spillover events and viral evolution. Vital to this understanding is the monitoring of bat migration patterns, which serves as a crucial tool for elucidating CoV ecology and epidemiology. Such efforts are essential for ongoing research endeavours aimed at mitigating the potential for future zoonotic disease outbreaks.<\/jats:p>","DOI":"10.3390\/v16030434","type":"journal-article","created":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T03:55:34Z","timestamp":1710215734000},"page":"434","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Presence of Alphacoronavirus in Tree- and Crevice-Dwelling Bats from Portugal"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4295-3788","authenticated-orcid":false,"given":"Mahima","family":"Hemnani","sequence":"first","affiliation":[{"name":"School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8384-0640","authenticated-orcid":false,"given":"Priscilla Gomes","family":"da Silva","sequence":"additional","affiliation":[{"name":"School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"},{"name":"Epidemiology Research Unit (EPIunit), Institute of Public Health, University of Porto, 4099-002 Porto, Portugal"},{"name":"Laborat\u00f3rio Para a Investiga\u00e7\u00e3o Integrativa e Translacional em Sa\u00fade Populacional (ITR), 4050-313 Porto, Portugal"},{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, 4099-002 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, 4099-002 Porto, Portugal"}]},{"given":"Gertrude","family":"Thompson","sequence":"additional","affiliation":[{"name":"School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"},{"name":"Biopolis-CIBIO\/InBIO Laborat\u00f3rio Associado, Campus de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-689X","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Poeta","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os Montes e Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Associated Laboratory for Green Chemistry (LAQV), Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Caparica, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Veterinary and Animal Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7118-4068","authenticated-orcid":false,"given":"Hugo","family":"Rebelo","sequence":"additional","affiliation":[{"name":"Biopolis-CIBIO\/InBIO Laborat\u00f3rio Associado, Campus de Vair\u00e3o, 4485-661 Vair\u00e3o, Portugal"},{"name":"cE3c\u2014Centre for Ecology, Evolution and Environmental Changes & CHANGE\u2014Global Change and Sustainability Institute, Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8769-8103","authenticated-orcid":false,"given":"Jo\u00e3o R.","family":"Mesquita","sequence":"additional","affiliation":[{"name":"School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"},{"name":"Epidemiology Research Unit (EPIunit), Institute of Public Health, University of Porto, 4099-002 Porto, Portugal"},{"name":"Laborat\u00f3rio Para a Investiga\u00e7\u00e3o Integrativa e Translacional em Sa\u00fade Populacional (ITR), 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.virusres.2006.01.017","article-title":"Nidovirales: Evolving the Largest RNA Virus Genome","volume":"117","author":"Gorbalenya","year":"2006","journal-title":"Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"veab050","DOI":"10.1093\/ve\/veab050","article-title":"Slippery When Wet: Cross-Species Transmission of Divergent Coronaviruses in Bony and Jawless Fish and the Evolutionary History of the Coronaviridae","volume":"7","author":"Miller","year":"2021","journal-title":"Virus Evol."},{"key":"ref_3","first-page":"001843","article-title":"ICTV Virus Taxonomy Profile: Coronaviridae","volume":"104","author":"Woo","year":"2023","journal-title":"J. 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