{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T10:35:18Z","timestamp":1768818918319,"version":"3.49.0"},"reference-count":91,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T00:00:00Z","timestamp":1582070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["GHTM, UID\/Multi\/04413\/2013"],"award-info":[{"award-number":["GHTM, UID\/Multi\/04413\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/CVT-CVT\/29073\/2017"],"award-info":[{"award-number":["PTDC\/CVT-CVT\/29073\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/121946\/2016"],"award-info":[{"award-number":["SFRH\/BD\/121946\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/122894\/2016"],"award-info":[{"award-number":["SFRH\/BD\/122894\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>The negative impact of ticks and tick-borne diseases on animals and human health is driving research to discover novel targets affecting both vectors and pathogens. The salivary glands are involved in feeding and pathogen transmission, thus are considered as a compelling target to focus research. In this study, proteomics approach was used to characterize Rhipicephalus bursa sialoproteome in response to Babesia ovis infection and blood feeding. Two potential tick protective antigens were identified and its influence in tick biological parameters and pathogen infection was evaluated. Results demonstrate that the R. bursa sialoproteome is highly affected by feeding but infection is well tolerated by tick cells. The combination of both stimuli shifts the previous scenario and a more evident pathogen manipulation can be suggested. Knockdown of ub2n led to a significative increase of infection in tick salivary glands but a brusque decrease in the progeny, revealing its importance in the cellular response to pathogen infection, which is worth pursuing in future studies. Additionally, an impact in the recovery rate of adults (62%), the egg production efficiency (45.75%), and the hatching rate (88.57 %) was detected. Building knowledge on vector and\/or pathogen interplay bridges the identification of protective antigens and the development of novel control strategies.<\/jats:p>","DOI":"10.3390\/vaccines8010091","type":"journal-article","created":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T03:20:03Z","timestamp":1582168803000},"page":"91","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Quantitative Proteomics Identifies Metabolic Pathways Affected by Babesia Infection and Blood Feeding in the Sialoproteome of the Vector Rhipicephalus bursa"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5259-1509","authenticated-orcid":false,"given":"Joana","family":"Couto","sequence":"first","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"},{"name":"Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM-IHMT-UNL), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4172-9079","authenticated-orcid":false,"given":"Margarita","family":"Villar","sequence":"additional","affiliation":[{"name":"SaBio, Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s\/n, 13005 Ciudad Real, Spain"},{"name":"Biochemistry Section, Faculty of Science and Chemical Technologies, and Regional Centre for Biomedical Research (CRIB), University of Castilla-La Mancha, 13071 Ciudad Real, Spain"}]},{"given":"Lourdes","family":"Mateos-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"SaBio, Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s\/n, 13005 Ciudad Real, Spain"},{"name":"UMR BIPAR, ANSES, INRA, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Universit\u00e9 Paris-Est, F-94700 Maisons-Alfort, France"}]},{"given":"Joana","family":"Ferrolho","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"},{"name":"Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM-IHMT-UNL), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"}]},{"given":"Gustavo","family":"Sanches","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"},{"name":"Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM-IHMT-UNL), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"}]},{"given":"Ana","family":"Sofia Santos","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Sa\u00fade Doutor Ricardo Jorge, Centro de Estudos de Vectores e Doen\u00e7as Infecciosas Dr. Francisco Cambournac (CEVDI), Av. \u00aa da Liberdade, 5, 2965-575 \u00c1guas de Moura, Portugal"},{"name":"Instituto de Sa\u00fade Ambiental, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal"}]},{"given":"Maria","family":"Santos-Silva","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Sa\u00fade Doutor Ricardo Jorge, Centro de Estudos de Vectores e Doen\u00e7as Infecciosas Dr. Francisco Cambournac (CEVDI), Av. \u00aa da Liberdade, 5, 2965-575 \u00c1guas de Moura, Portugal"},{"name":"Instituto de Sa\u00fade Ambiental, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal"}]},{"given":"Jo\u00e3o","family":"Nobre","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta da Fonte Boa, Vale de Santar\u00e9m, 2005-048 Santar\u00e9m, Portugal"}]},{"given":"Olga","family":"Moreira","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta da Fonte Boa, Vale de Santar\u00e9m, 2005-048 Santar\u00e9m, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5512-9093","authenticated-orcid":false,"given":"Sandra","family":"Antunes","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"},{"name":"Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM-IHMT-UNL), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7383-9649","authenticated-orcid":false,"given":"Jos\u00e9","family":"de la Fuente","sequence":"additional","affiliation":[{"name":"SaBio, Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s\/n, 13005 Ciudad Real, Spain"},{"name":"Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4570-2060","authenticated-orcid":false,"given":"Ana","family":"Domingos","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"},{"name":"Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM-IHMT-UNL), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3389\/fcimb.2017.00281","article-title":"The Essential Role of Tick Salivary Glands and Saliva in Tick Feeding and Pathogen Transmission","volume":"7","author":"Kazimirova","year":"2017","journal-title":"Front. 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