{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T15:33:49Z","timestamp":1770564829971,"version":"3.49.0"},"reference-count":39,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T00:00:00Z","timestamp":1676419200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Immunolabeling on Romanowsky-stained cytology (RSC) slides can be used, although there is limited evidence of its suitability for phenotyping canine and feline lymphomas. A comparison with matched cell blocks (CB) is missing. Immunolabeling on RSC and CB was compared for lymphoid markers (CD3 and PAX5) in 53 lymphomas and 4 chylous effusions from dogs and cats. The influence of pre-analytical variables (species, time of archive, type of specimens and coverslipping) and the interobserver agreement among the 2 observers was assessed. Fewer CD3+ lymphocytes were identified in RSC, while the PAX5 positivity by RSC and CB had a substantial agreement. Immunodetection of CD3 and the diagnosis of a T-cell population on RSC was more difficult. Lower intensity and higher background were noted in RSC. Immunophenotyping was inconclusive in 54% RSC and 19% CB. The interobserver reproducibility of immunophenotyping on CB was substantial, being higher than in RSC. The immunolabeling performance on the RSC of effusion and feline samples was unsatisfactory. The detection of lymphoid markers, especially membranous antigens in retrospective RSC, is affected by the pre-analytical variables: species, time of the archive, and type of specimens. CB are a more consistent type of sample for immunophenotyping purposes.<\/jats:p>","DOI":"10.3390\/vetsci10020157","type":"journal-article","created":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T03:09:21Z","timestamp":1676430561000},"page":"157","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Detection of Lymphoid Markers (CD3 and PAX5) for Immunophenotyping in Dogs and Cats: Comparison of Stained Cytology Slides and Matched Cell Blocks"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0206-6903","authenticated-orcid":false,"given":"Filipe","family":"Sampaio","sequence":"first","affiliation":[{"name":"Cytology and Hematology Diagnostic Services, Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (U.Porto), Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"},{"name":"Laborat\u00f3rio INNO, 4710-503 Braga, Portugal"}]},{"given":"Carla","family":"Marrinhas","sequence":"additional","affiliation":[{"name":"Cytology and Hematology Diagnostic Services, Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (U.Porto), Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"},{"name":"Hospital do Baixo Vouga, OneVet Group, 3750-742 \u00c1gueda, Portugal"}]},{"given":"Lu\u00edsa","family":"Fonte Oliveira","sequence":"additional","affiliation":[{"name":"Cytology and Hematology Diagnostic Services, Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (U.Porto), Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"},{"name":"Anicura CHV Porto, 4100-320 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4978-5358","authenticated-orcid":false,"given":"Fernanda","family":"Malh\u00e3o","sequence":"additional","affiliation":[{"name":"Cytology and Hematology Diagnostic Services, Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (U.Porto), Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"given":"C\u00e9lia","family":"Lopes","sequence":"additional","affiliation":[{"name":"Cytology and Hematology Diagnostic Services, Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (U.Porto), Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4346-2259","authenticated-orcid":false,"given":"Hugo","family":"Greg\u00f3rio","sequence":"additional","affiliation":[{"name":"Anicura CHV Porto, 4100-320 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2909-4608","authenticated-orcid":false,"given":"Carla","family":"Correia-Gomes","sequence":"additional","affiliation":[{"name":"Animal Health Ireland, Carrick on Shannon Co., N41 WN27 Leitrim, Ireland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5502-5991","authenticated-orcid":false,"given":"Ricardo","family":"Marcos","sequence":"additional","affiliation":[{"name":"Cytology and Hematology Diagnostic Services, Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (U.Porto), Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"given":"Mario","family":"Caniatti","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS), Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1350-4254","authenticated-orcid":false,"given":"Marta","family":"Santos","sequence":"additional","affiliation":[{"name":"Cytology and Hematology Diagnostic Services, Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (U.Porto), Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"},{"name":"Oncology Research, UMIB\u2014Unit for Multidisciplinary Research in Biomedicine, ICBAS, University of Porto, 4050-313 Porto, Portugal"},{"name":"ITR\u2014Laboratory for Integrative and Translational Research in Population Health, 4050-600 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1354\/vp.39-1-92","article-title":"Canine T-cell lymphomas: A morphological, immunological, and clinical study of 46 new cases","volume":"39","author":"Ponce","year":"2002","journal-title":"Vet. 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