{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T05:24:44Z","timestamp":1761110684566,"version":"build-2065373602"},"reference-count":135,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:00:00Z","timestamp":1700784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PT national funds","award":["UIDB\/50006\/2020|UIDP\/50006\/2020","UIDB\/04033\/2020","UIDB\/CVT\/00772\/2020","LA\/P\/0059\/2020","SFRH\/BD\/148830\/2019"],"award-info":[{"award-number":["UIDB\/50006\/2020|UIDP\/50006\/2020","UIDB\/04033\/2020","UIDB\/CVT\/00772\/2020","LA\/P\/0059\/2020","SFRH\/BD\/148830\/2019"]}]},{"name":"FCT","award":["UIDB\/50006\/2020|UIDP\/50006\/2020","UIDB\/04033\/2020","UIDB\/CVT\/00772\/2020","LA\/P\/0059\/2020","SFRH\/BD\/148830\/2019"],"award-info":[{"award-number":["UIDB\/50006\/2020|UIDP\/50006\/2020","UIDB\/04033\/2020","UIDB\/CVT\/00772\/2020","LA\/P\/0059\/2020","SFRH\/BD\/148830\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Adipose tissue-derived stromal cells (ADSCs) have generated considerable interest in the field of veterinary medicine, particularly for their potential in therapeutic strategies focused on bone regeneration. These cells possess unique biological characteristics, including their regenerative capacity and their ability to produce bioactive molecules. However, it is crucial to recognize that the characteristics of ADSCs can vary depending on the animal species and the site from which they are derived, such as the subcutaneous and visceral regions (SCAT and VAT, respectively). Thus, the present work aimed to comprehensively review the different traits of ADSCs isolated from diverse anatomical sites in companion animals, i.e., dogs, cats, and horses, in terms of immunophenotype, morphology, proliferation, and osteogenic differentiation potential. The findings indicate that the immunophenotype, proliferation, and osteogenic potential of ADSCs differ according to tissue origin and species. Generally, the proliferation rate is higher in VAT-derived ADSCs in dogs and horses, whereas in cats, the proliferation rate appears to be similar in both cells isolated from SCAT and VAT regions. In terms of osteogenic differentiation potential, VAT-derived ADSCs demonstrate the highest capability in cats, whereas SCAT-derived ADSCs exhibit superior potential in horses. Interestingly, in dogs, VAT-derived cells appear to have greater potential than those isolated from SCAT. Within the VAT, ADSCs derived from the falciform ligament and omentum show increased osteogenic potential, compared to cells isolated from other anatomical locations. Consequently, considering these disparities, optimizing isolation protocols becomes pivotal, tailoring them to the specific target species and therapeutic aims, and judiciously selecting the anatomical site for ADSC isolation. This approach holds promise to enhance the efficacy of ADSCs-based bone regenerative therapies.<\/jats:p>","DOI":"10.3390\/vetsci10120673","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T10:10:39Z","timestamp":1700820639000},"page":"673","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells\u2019 Biological Profile and Osteogenic Potential in Companion Animals"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9985-4529","authenticated-orcid":false,"given":"Carla","family":"Ferreira-Baptista","sequence":"first","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"BoneLab\u2014Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"REQUIMTE\/LAQV, University of Porto, 4100-007 Porto, Portugal"},{"name":"REQUIMTE\/LAQV, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6872-4051","authenticated-orcid":false,"given":"Rita","family":"Ferreira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9391-9574","authenticated-orcid":false,"given":"Maria Helena","family":"Fernandes","sequence":"additional","affiliation":[{"name":"BoneLab\u2014Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"REQUIMTE\/LAQV, University of Porto, 4100-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5365-2123","authenticated-orcid":false,"given":"Pedro Sousa","family":"Gomes","sequence":"additional","affiliation":[{"name":"BoneLab\u2014Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"REQUIMTE\/LAQV, University of Porto, 4100-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4879-8624","authenticated-orcid":false,"given":"Bruno","family":"Cola\u00e7o","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"REQUIMTE\/LAQV, University of Porto, 4100-007 Porto, Portugal"},{"name":"CECAV\u2014Animal and Veterinary Research Centre UTAD, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1002\/stem.269","article-title":"Mesenchymal stromal cells: Current understanding and clinical status","volume":"28","author":"Salem","year":"2010","journal-title":"Stem Cells"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Szydlarska, J., Weiss, C., and Marycz, K. (2018). The Effect of Methyl-\u03b2-cyclodextrin on Apoptosis, Proliferative Activity, and Oxidative Stress in Adipose-Derived Mesenchymal Stromal Cells of Horses Suffering from Metabolic Syndrome (EMS). Molecules, 23.","DOI":"10.3390\/molecules23020287"},{"key":"ref_3","first-page":"747","article-title":"Comprehensive characterization of human adipose tissue-derived stem cells expanded in vitro","volume":"68","author":"Varga","year":"2013","journal-title":"Biol. Sect. Zool."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1089\/cell.2012.0004","article-title":"Human Mesenchymal Stem Cells Derived From Limb Bud Can Differentiate into All Three Embryonic Germ Layers Lineages","volume":"14","author":"Jiao","year":"2012","journal-title":"Cell. Reprogram."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s11259-017-9705-x","article-title":"Stem cell factor supports migration in canine mesenchymal stem cells","volume":"42","author":"Enciso","year":"2018","journal-title":"Vet. Res. Commun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.theriogenology.2017.09.035","article-title":"Equine mesenchymal stem cells derived from endometrial or adipose tissue share significant biological properties, but have distinctive pattern of surface markers and migration","volume":"106","author":"Cabezas","year":"2018","journal-title":"Theriogenology"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Falomo, M.E., Ferroni, L., Tocco, I., Gardin, C., and Zavan, B. (2015). Immunomodulatory Role of Adipose-Derived Stem Cells on Equine Endometriosis. BioMed Res. Int., 2015.","DOI":"10.1155\/2015\/141485"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.2460\/ajvr.73.8.1305","article-title":"Isolation, characterization, and in vitro proliferation of canine mesenchymal stem cells derived from bone marrow, adipose tissue, muscle, and periosteum","volume":"73","author":"Kisiel","year":"2012","journal-title":"Am. J. Vet. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Villatoro, A.J., Fern\u00e1ndez, V., Claros, S., Rico-Llanos, G.A., Becerra, J., and Andrades, J.A. (2015). Use of adipose-derived mesenchymal stem cells in keratoconjunctivitis sicca in a canine model. BioMed Res. Int., 2015.","DOI":"10.1155\/2015\/527926"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Dias, I.E., Pinto, P.O., Barros, L.C., Viegas, C.A., Dias, I.R., and Carvalho, P.P. (2019). Mesenchymal stem cells therapy in companion animals: Useful for immune-mediated diseases?. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-2087-2"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"101442","DOI":"10.1016\/j.scr.2019.101442","article-title":"Analysis of mesenchymal cells (MSCs) from bone marrow, synovial fluid and mesenteric, neck and tail adipose tissue sources from equines","volume":"37","author":"Olmeo","year":"2019","journal-title":"Stem Cell Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1186\/s13287-017-0639-6","article-title":"In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: A donor-matched comparative study","volume":"8","author":"Bearden","year":"2017","journal-title":"Stem Cell Res. Ther."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"299","DOI":"10.4142\/jvs.2012.13.3.299","article-title":"Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues, bone marrow, umbilical cord blood, and Wharton\u2019s jelly for treating bone defects","volume":"13","author":"Kang","year":"2012","journal-title":"J. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/S0301-472X(02)00864-0","article-title":"Isolation and characterization of multipotential mesenchymal stem cells from feline bone marrow","volume":"30","author":"Martin","year":"2002","journal-title":"Exp. Hematol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"790","DOI":"10.2460\/ajvr.74.5.790","article-title":"Characterization and osteogenic potential of equine muscle tissue\u2013 and periosteal tissue\u2013derived mesenchymal stem cells in comparison with bone marrow\u2013 and adipose tissue\u2013derived mesenchymal stem cells","volume":"74","author":"Radtke","year":"2013","journal-title":"Am. J. Vet. Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Sasaki, A., Mizuno, M., Ozeki, N., Katano, H., Otabe, K., Tsuji, K., Koga, H., Mochizuki, M., and Sekiya, I. (2018). Canine mesenchymal stem cells from synovium have a higher chondrogenic potential than those from infrapatellar fat pad, adipose tissue, and bone marrow. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0202922"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1177\/1098612X11429224","article-title":"In vitro comparison of feline bone marrow-derived and adipose tissue-derived mesenchymal stem cells","volume":"14","author":"Webb","year":"2011","journal-title":"J. Feline Med. Surg."},{"key":"ref_18","first-page":"2097","article-title":"Characteristics and multi-lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff","volume":"18","author":"Zhang","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1634\/stemcells.2005-0342","article-title":"Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue","volume":"24","author":"Kern","year":"2006","journal-title":"Stem Cells"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1292\/jvms.17-0563","article-title":"Altered properties of feline adipose-derived mesenchymal stem cells during continuous in vitro cultivation","volume":"80","author":"Lee","year":"2018","journal-title":"J. Vet. Med. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Mensing, N., Gasse, H., Hambruch, N., Haeger, J.-D., Pfarrer, C., and Staszyk, C. (2011). Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse. BMC Vet. Res., 7.","DOI":"10.1186\/1746-6148-7-42"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Presen, D.M., Traweger, A., Gimona, M., and Redl, H. (2019). Mesenchymal Stromal Cell-Based Bone Regeneration Therapies: From Cell Transplantation and Tissue Engineering to Therapeutic Secretomes and Extracellular Vesicles. Front. Bioeng. Biotechnol., 7.","DOI":"10.3389\/fbioe.2019.00352"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1292\/jvms.70.723","article-title":"Generation of Neuronal-Like Cells from Umbilical Cord Blood-Derived Mesenchymal Stem Cells of a RFP-Transgenic Cloned Cat","volume":"70","author":"Jin","year":"2008","journal-title":"J. Vet. Med. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.3109\/14653249.2011.602338","article-title":"Intradermal injections of equine allogeneic umbilical cord-derived mesenchymal stem cells are well tolerated and do not elicit immediate or delayed hypersensitivity reactions","volume":"13","author":"Carrade","year":"2011","journal-title":"Cytotherapy"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.tvjl.2016.08.009","article-title":"Isolation and characterisation of peripheral blood-derived feline mesenchymal stem cells","volume":"216","author":"Sato","year":"2016","journal-title":"Vet. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1983025","DOI":"10.1155\/2018\/1983025","article-title":"Evaluation of the Curative Effect of Umbilical Cord Mesenchymal Stem Cell Therapy for Knee Arthritis in Dogs Using Imaging Technology","volume":"2018","author":"Zhang","year":"2018","journal-title":"Stem Cells Int."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Koch, T.G., Heerkens, T., Thomsen, P.D., and Betts, D.H. (2007). Isolation of mesenchymal stem cells from equine umbilical cord blood. BMC Biotechnol., 7.","DOI":"10.1186\/1472-6750-7-26"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4279","DOI":"10.1091\/mbc.e02-02-0105","article-title":"Human adipose tissue is a source of multipotent stem cells","volume":"13","author":"Zuk","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s12015-019-09932-0","article-title":"Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders","volume":"16","author":"Naem","year":"2020","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Prz\u0105dka, P., Buczak, K., Frejlich, E., G\u0105sior, L., Suliga, K., and Kie\u0142bowicz, Z. (2021). The Role of Mesenchymal Stem Cells (MSCs) in Veterinary Medicine and Their Use in Musculoskeletal Disorders. Biomolecules, 11.","DOI":"10.3390\/biom11081141"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"81","DOI":"10.3389\/fvets.2016.00081","article-title":"A Prospective, Randomized, Masked, and Placebo-Controlled Efficacy Study of Intraarticular Allogeneic Adipose Stem Cells for the Treatment of Osteoarthritis in Dogs","volume":"3","author":"Harman","year":"2016","journal-title":"Front. Vet. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.rvsc.2018.01.015","article-title":"Advanced nutritional and stem cells approaches to prevent equine metabolic syndrome","volume":"118","author":"Marycz","year":"2018","journal-title":"Res. Vet. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.ijom.2012.10.012","article-title":"A comparison of tissue-engineered bone from adipose-derived stem cell with autogenous bone repair in maxillary alveolar cleft model in dogs","volume":"42","author":"Pourebrahim","year":"2013","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wysong, A., Ortiz, P., Bittel, D., Ott, L., Karanu, F., Filla, M., and Stehno-Bittel, L. (2021). Viability, yield and expansion capability of feline MSCs obtained from subcutaneous and reproductive organ adipose depots. BMC Vet. Res., 17.","DOI":"10.1186\/s12917-021-02948-0"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"610240","DOI":"10.3389\/fvets.2020.610240","article-title":"Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells\/Medicinal Signaling Cells with Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential","volume":"7","author":"Voga","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.4252\/wjsc.v3.i4.25","article-title":"Adipose-derived stromal cells: Their identity and uses in clinical trials, an update","volume":"3","author":"Casteilla","year":"2011","journal-title":"World J. Stem Cells"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1080\/21623945.2017.1372871","article-title":"Fifty shades of white: Understanding heterogeneity in white adipose stem cells","volume":"6","author":"Cleal","year":"2017","journal-title":"Adipocyte"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Trachsel, D.S., Stage, H.J., Rausch, S., Trappe, S., S\u00f6llig, K., Sponder, G., Merle, R., Aschenbach, J.R., and Gehlen, H. (2022). Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal\/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine. Animals, 12.","DOI":"10.3390\/ani12162049"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s12015-015-9634-4","article-title":"Effects of Cryopreservation on Canine Multipotent Stromal Cells from Subcutaneous and Infrapatellar Adipose Tissue","volume":"12","author":"Duan","year":"2016","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e215","DOI":"10.1038\/emm.2016.5","article-title":"Heterogeneity of white adipose tissue: Molecular basis and clinical implications","volume":"48","author":"Kwok","year":"2016","journal-title":"Exp. Mol. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"104","DOI":"10.15283\/ijsc.2012.5.2.104","article-title":"Adipose stem cells as alternatives for bone marrow mesenchymal stem cells in oral ulcer healing","volume":"5","author":"Menoufy","year":"2012","journal-title":"Int. J. Stem Cells"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Bahamondes, F., Flores, E., Cattaneo, G., Bruna, F., and Conget, P. (2017). Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1053-0"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.rvsc.2010.10.012","article-title":"Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia","volume":"92","author":"Chung","year":"2012","journal-title":"Res. Vet. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s12015-015-9638-0","article-title":"Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype","volume":"12","author":"Clark","year":"2016","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"9127","DOI":"10.1038\/s41598-022-13114-3","article-title":"In vitro generation of transplantable insulin-producing cells from canine adipose-derived mesenchymal stem cells","volume":"12","author":"Rodprasert","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/14653240600855905","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement","volume":"8","author":"Dominici","year":"2006","journal-title":"Cytotherapy"},{"key":"ref_47","first-page":"3053759","article-title":"Safety of Allogeneic Canine Adipose Tissue-Derived Mesenchymal Stem Cell Intraspinal Transplantation in Dogs with Chronic Spinal Cord Injury","volume":"2017","author":"Ramos","year":"2017","journal-title":"Stem Cells Int."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Hendawy, H., Uemura, A., Ma, D., Namiki, R., Samir, H., Ahmed, M.F., Elfadadny, A., El-Husseiny, H.M., Chieh-Jen, C., and Tanaka, R. (2021). Tissue Harvesting Site Effect on the Canine Adipose Stromal Vascular Fraction Quantity and Quality. Animals, 11.","DOI":"10.3390\/ani11020460"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.theriogenology.2013.09.018","article-title":"Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos","volume":"81","author":"Kim","year":"2014","journal-title":"Theriogenology"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.3727\/096368915X689532","article-title":"Osteogenic Ability of Canine Adipose-Derived Mesenchymal Stromal Cell Sheets in Relation to Culture Time","volume":"25","author":"Kuk","year":"2016","journal-title":"Cell Transplant."},{"key":"ref_51","first-page":"294","article-title":"Evaluation of adipose-derived stromal vascular fraction from the lateral tailhead, inguinal region, and mesentery of horses","volume":"80","author":"Metcalf","year":"2016","journal-title":"Can. J. Vet. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1089\/ten.tea.2007.0207","article-title":"Isolation and Characterization of Canine Adipose\u2013Derived Mesenchymal Stem Cells","volume":"14","author":"Neupane","year":"2008","journal-title":"Tissue Eng. Part A"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.3727\/096368912X657981","article-title":"Donor-Matched Functional and Molecular Characterization of Canine Mesenchymal Stem Cells Derived from Different Origins","volume":"22","author":"Ock","year":"2013","journal-title":"Cell Transplant."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s13018-020-02178-4","article-title":"Mesenchymal stem cells genetically engineered to express platelet-derived growth factor and heme oxygenase-1 ameliorate osteoarthritis in a canine model","volume":"16","author":"Oh","year":"2021","journal-title":"J. Orthop. Surg. Res."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Rashid, U., Yousaf, A., Yaqoob, M., Saba, E., Moaeen-ud-Din, M., Waseem, S., Becker, S.K., Sponder, G., Aschenbach, J.R., and Sandhu, M.A. (2021). Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources. BMC Vet. Res., 17.","DOI":"10.1186\/s12917-021-03100-8"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1007\/s12015-012-9397-0","article-title":"Effect of Anatomical Origin and Cell Passage Number on the Stemness and Osteogenic Differentiation Potential of Canine Adipose-Derived Stem Cells","volume":"8","author":"Requicha","year":"2012","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1302\/2046-3758.1010.BJR-2020-0540.R1","article-title":"Clinical outcomes following intra-articular injection of autologous adipose-derived mesenchymal stem cells for the treatment of osteoarthritis in dogs characterized by weight-bearing asymmetry","volume":"10","author":"Black","year":"2021","journal-title":"Bone Jt. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.rvsc.2011.08.010","article-title":"Effects of different media on proliferation and differentiation capacity of canine, equine and porcine adipose derived stem cells","volume":"93","author":"Schwarz","year":"2012","journal-title":"Res. Vet. Sci."},{"key":"ref_59","first-page":"82","article-title":"Comparison of Mesenchymal Stem Cell Surface Markers from Bone Marrow Aspirates and Adipose Stromal Vascular Fraction Sites","volume":"2","author":"Sullivan","year":"2015","journal-title":"Front. Vet. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Takemitsu, H., Zhao, D., Yamamoto, I., Harada, Y., Michishita, M., and Arai, T. (2012). Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells. BMC Vet. Res., 8.","DOI":"10.1186\/1746-6148-8-150"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1609876","DOI":"10.1155\/2019\/1609876","article-title":"Comparison of Properties of Stem Cells Isolated from Adipose Tissue and Lipomas in Dogs","volume":"2019","author":"Teshima","year":"2019","journal-title":"Stem Cells Int."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"12335","DOI":"10.1073\/pnas.0604849103","article-title":"Osteogenic differentiation of mouse adipose-derived adult stromal cells requires retinoic acid and bone morphogenetic protein receptor type IB signaling","volume":"103","author":"Wan","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e63","DOI":"10.4142\/jvs.2021.22.e63","article-title":"Immunosuppression-enhancing effect of the administration of allogeneic canine adipose-derived mesenchymal stem cells (cA-MSCs) compared with autologous cA-MSCs in vitro","volume":"22","author":"Wi","year":"2021","journal-title":"J. Vet. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"13","DOI":"10.4142\/jvs.2018.19.1.13","article-title":"Comparison of the characteristics of canine adipose tissue-derived mesenchymal stem cells extracted from different sites and at different passage numbers","volume":"19","author":"Yaneselli","year":"2018","journal-title":"J. Vet. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Ferreira-Baptista, C., Queir\u00f3s, A., Ferreira, R., Fernandes, M.H., Cola\u00e7o, B., and Gomes, P.S. (2022). The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells-A Comparative Analysis between Two Osteogenic Induction Programs. Bioengineering, 9.","DOI":"10.3390\/bioengineering9120810"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Teshima, T., Matsumoto, H., and Koyama, H. (2018). Soluble factors from adipose tissue-derived mesenchymal stem cells promote canine hepatocellular carcinoma cell proliferation and invasion. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0191539"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"3892514","DOI":"10.1155\/2017\/3892514","article-title":"Allogenic Adipose Tissue-Derived Mesenchymal Stem Cells Ameliorate Acute Hepatic Injury in Dogs","volume":"2017","author":"Teshima","year":"2017","journal-title":"Stem Cells Int."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"383","DOI":"10.3389\/fvets.2019.00383","article-title":"Comparison of the Efficacy of Surgical Decompression Alone and Combined with Canine Adipose Tissue-Derived Stem Cell Transplantation in Dogs with Acute Thoracolumbar Disk Disease and Spinal Cord Injury","volume":"6","author":"Bach","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/rda.12885","article-title":"Isolation, proliferation and characterization of endometrial canine stem cells","volume":"52","author":"Grolli","year":"2017","journal-title":"Reprod. Domest. Anim."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.neulet.2018.12.004","article-title":"Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI","volume":"696","author":"Krueger","year":"2019","journal-title":"Neurosci. Lett."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1002\/jcb.25610","article-title":"Canine Adipose-Derived Stem Cells: Purinergic Characterization and Neurogenic Potential for Therapeutic Applications","volume":"118","author":"Roszek","year":"2017","journal-title":"J. Cell. Biochem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1002\/vms3.816","article-title":"Laparoscopic adipose-derived stem cell harvest technique with bipolar sealing device: Outcome in 12 dogs","volume":"8","author":"Buote","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/joa.13758","article-title":"Retinoic acid induces the osteogenic differentiation of cat adipose tissue-derived stromal cells from distinct anatomical sites","volume":"242","author":"Ferreira","year":"2023","journal-title":"J. Anat."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.reth.2020.03.013","article-title":"Basic fibroblast growth factor enhances proliferation and hepatocyte growth factor expression of feline mesenchymal stem cells","volume":"15","author":"Fujimoto","year":"2020","journal-title":"Regen. Ther."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.tvjl.2013.10.033","article-title":"Phenotypic and functional properties of feline dedifferentiated fat cells and adipose-derived stem cells","volume":"199","author":"Kono","year":"2014","journal-title":"Vet. J."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Li, D., Luo, H., Ruan, H., Chen, Z., Chen, S., Wang, B., and Xie, Y. (2021). Isolation and identification of exosomes from feline plasma, urine and adipose-derived mesenchymal stem cells. BMC Vet. Res., 17.","DOI":"10.1186\/s12917-021-02960-4"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1111\/jvim.13655","article-title":"Safety of Intraperitoneal Injection of Adipose Tissue-Derived Autologous Mesenchymal Stem Cells in Cats","volume":"30","author":"Parys","year":"2016","journal-title":"J. Vet. Intern. Med."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/scrt198","article-title":"Safety and efficacy of intravenous infusion of allogeneic cryopreserved mesenchymal stem cells for treatment of chronic kidney disease in cats: Results of three sequential pilot studies","volume":"4","author":"Quimby","year":"2013","journal-title":"Stem Cell Res. Ther."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Teshima, T., Yasumura, Y., Suzuki, R., and Matsumoto, H. (2022). Antiviral Effects of Adipose Tissue-Derived Mesenchymal Stem Cells Secretome against Feline Calicivirus and Feline Herpesvirus Type 1. Viruses, 14.","DOI":"10.3390\/v14081687"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Villatoro, A.J., Claros, S., Fern\u00e1ndez, V., Alcoholado, C., Fari\u00f1as, F., Moreno, A., Becerra, J., and Andrades, J.A. (2018). Safety and efficacy of the mesenchymal stem cell in feline eosinophilic keratitis treatment. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1413-4"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Villatoro, A.J., Mart\u00edn-Astorga, M.D.C., Alcoholado, C., C\u00e1rdenas, C., Fari\u00f1as, F., Becerra, J., and Visser, R. (2021). Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis. Animals, 11.","DOI":"10.3390\/ani11082466"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1177\/1098612X15576980","article-title":"Assessment of intravenous adipose-derived allogeneic mesenchymal stem cells for the treatment of feline chronic kidney disease: A randomized, placebo-controlled clinical trial in eight cats","volume":"18","author":"Quimby","year":"2015","journal-title":"J. Feline Med. Surg."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.vetimm.2017.07.013","article-title":"Immunomodulatory effects of soluble factors secreted by feline adipose tissue-derived mesenchymal stem cells","volume":"191","author":"Chae","year":"2017","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1186\/s13287-019-1429-0","article-title":"Investigation of stemness and multipotency of equine adipose-derived mesenchymal stem cells (ASCs) from different fat sources in comparison with lipoma","volume":"10","author":"Arnhold","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Bagge, J., Berg, L.C., Janes, J., and MacLeod, J.N. (2022). Donor age effects on in vitro chondrogenic and osteogenic differentiation performance of equine bone marrow- and adipose tissue-derived mesenchymal stromal cells. BMC Vet. Res., 18.","DOI":"10.1186\/s12917-022-03475-2"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/scrt414","article-title":"Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: Immunophenotypic characterization and differentiation potential","volume":"5","author":"Barberini","year":"2014","journal-title":"Stem Cell Res. Ther."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.rvsc.2022.08.002","article-title":"Engineered nanoparticles toxicity on adipose tissue derived mesenchymal stem cells: A preliminary investigation","volume":"152","author":"Cacciamali","year":"2022","journal-title":"Res. Vet. Sci."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3727\/096368915X687822","article-title":"Comparative Characterization of Human and Equine Mesenchymal Stromal Cells: A Basis for Translational Studies in the Equine Model","volume":"25","author":"Hillmann","year":"2016","journal-title":"Cell Transpl."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"299","DOI":"10.4142\/jvs.2017.18.3.299","article-title":"Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells","volume":"18","author":"Kim","year":"2017","journal-title":"J. Vet. Sci."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"141","DOI":"10.4142\/jvs.2017.18.2.141","article-title":"Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells","volume":"18","author":"Lee","year":"2017","journal-title":"J. Vet. Sci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.1111\/jcmm.12932","article-title":"Equine metabolic syndrome impairs adipose stem cells osteogenic differentiation by predominance of autophagy over selective mitophagy","volume":"20","author":"Marycz","year":"2016","journal-title":"J. Cell. Mol. Med."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"392","DOI":"10.3389\/fimmu.2016.00392","article-title":"Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFN\u03b3-Primed Equine Mesenchymal Stem Cells","volume":"7","author":"Maumus","year":"2016","journal-title":"Front. Immunol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1002\/cyto.a.22491","article-title":"Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: An approach toward a standardized definition","volume":"85","author":"Paebst","year":"2014","journal-title":"Cytom. Part A"},{"key":"ref_94","first-page":"290","article-title":"Application of a novel sorting system for equine mesenchymal stem cells (MSCs)","volume":"78","author":"Radtke","year":"2014","journal-title":"Can. J. Vet. Res."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1111\/j.2042-3306.2010.00353.x","article-title":"Comparative study of equine bone marrow and adipose tissue-derived mesenchymal stromal cells","volume":"44","author":"Ranera","year":"2012","journal-title":"Equine Vet. J."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Stage, H.J., Trappe, S., S\u00f6llig, K., Trachsel, D.S., Kirsch, K., Zieger, C., Merle, R., Aschenbach, J.R., and Gehlen, H. (2023). Multilineage Differentiation Potential of Equine Adipose-Derived Stromal\/Stem Cells from Different Sources. Animals, 13.","DOI":"10.3390\/ani13081352"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.2460\/ajvr.71.10.1237","article-title":"Comparison of the osteogenic potential of equine mesenchymal stem cells from bone marrow, adipose tissue, umbilical cord blood, and umbilical cord tissue","volume":"71","author":"Toupadakis","year":"2010","journal-title":"Am. J. Vet. Res."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/j.1532-950X.2007.00313.x","article-title":"Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells","volume":"36","author":"Vidal","year":"2007","journal-title":"Vet. Surg."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"279","DOI":"10.3727\/096368909X481764","article-title":"Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells","volume":"19","author":"Vieira","year":"2010","journal-title":"Cell Transplant."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/cpr.12271","article-title":"Primordial germ cell-like cells derived from canine adipose mesenchymal stem cells","volume":"49","author":"Wei","year":"2016","journal-title":"Cell Prolif."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"37","DOI":"10.22203\/eCM.v025a03","article-title":"CD73\/5\u2032-ecto-nucleotidase acts as a regulatory factor in osteo-\/chondrogenic differentiation of mechanically stimulated mesenchymal stromal cells","volume":"25","author":"Ode","year":"2013","journal-title":"Eur. Cells Mater."},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Anderson, P., Carrillo-G\u00e1lvez, A.B., Garc\u00eda-P\u00e9rez, A., Cobo, M., and Mart\u00edn, F. (2013). CD105 (endoglin)-negative murine mesenchymal stromal cells define a new multipotent subpopulation with distinct differentiation and immunomodulatory capacities. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0076979"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"101133","DOI":"10.1016\/j.bonr.2021.101133","article-title":"Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo","volume":"15","author":"Kimura","year":"2021","journal-title":"Bone Rep."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1089\/ten.tea.2012.0370","article-title":"CD90 (Thy-1)-positive selection enhances osteogenic capacity of human adipose-derived stromal cells","volume":"19","author":"Chung","year":"2013","journal-title":"Tissue Eng. Part A"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1369\/jhc.2010.955393","article-title":"Localization of Thy-1-positive cells in the perichondrium during endochondral ossification","volume":"58","author":"Nakamura","year":"2010","journal-title":"J. Histochem. Cytochem. Off. J. Histochem. Soc."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Alves, E.G.L., Serakides, R., Boeloni, J.N., Rosado, I.R., Ocarino, N.M., Oliveira, H.P., G\u00f3es, A.M., and Rezende, C.M.F. (2014). Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs. BMC Vet. Res., 10.","DOI":"10.1186\/s12917-014-0190-y"},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Villatoro, A.J., Mart\u00edn-Astorga, M.D.C., Alcoholado, C., Kazantseva, L., C\u00e1rdenas, C., Fari\u00f1as, F., Becerra, J., and Visser, R. (2022). Secretory Profile of Adipose-Tissue-Derived Mesenchymal Stem Cells from Cats with Calicivirus-Positive Severe Chronic Gingivostomatitis. Viruses, 14.","DOI":"10.3390\/v14061146"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s11259-011-9480-z","article-title":"Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells","volume":"35","author":"Raabe","year":"2011","journal-title":"Vet. Res. Commun."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1186\/s13287-018-1041-8","article-title":"Mapping of equine mesenchymal stromal cell surface proteomes for identification of specific markers using proteomics and gene expression analysis: An in vitro cross-sectional study","volume":"9","author":"Bundgaard","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.tice.2017.07.003","article-title":"Bioactive glass ceramic nanoparticles-coated poly(l-lactic acid) scaffold improved osteogenic differentiation of adipose stem cells in equine","volume":"49","author":"Mahdavi","year":"2017","journal-title":"Tissue Cell"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1097\/PRS.0b013e31821e6e49","article-title":"Differences in Osteogenic Differentiation of Adipose-Derived Stromal Cells from Murine, Canine, and Human Sources In Vitro and In Vivo","volume":"128","author":"Levi","year":"2011","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/scrt328","article-title":"Effects of dexamethasone, ascorbic acid and \u03b2-glycerophosphate on the osteogenic differentiation of stem cells in vitro","volume":"4","author":"Langenbach","year":"2013","journal-title":"Stem Cell Res. Ther."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"659","DOI":"10.3892\/mmr.2013.1811","article-title":"Effects of the combination of dexamethasone and fibroblast growth factor-2 on differentiation of osteoprecursor cells","volume":"9","author":"Park","year":"2014","journal-title":"Mol. Med. Rep."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10856-020-06475-6","article-title":"Effect of dexamethasone as osteogenic supplementation in in vitro osteogenic differentiation of stem cells from human exfoliated deciduous teeth","volume":"32","author":"Sordi","year":"2021","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"31612","DOI":"10.18632\/oncotarget.15779","article-title":"A novel bioactive osteogenesis scaffold delivers ascorbic acid, \u03b2-glycerophosphate, and dexamethasone in vivo to promote bone regeneration","volume":"8","author":"Wang","year":"2017","journal-title":"Oncotarget"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"e20180317","DOI":"10.1590\/1678-7757-2018-0317","article-title":"Retinoic acid increases the effect of bone morphogenetic protein type 2 on osteogenic differentiation of human adipose-derived stem cells","volume":"27","author":"Cruz","year":"2019","journal-title":"J. Appl. Oral Sci."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1089\/ten.2006.0283","article-title":"Refining Retinoic Acid Stimulation for Osteogenic Differentiation of Murine Adipose-Derived Adult Stromal Cells","volume":"13","author":"Wan","year":"2007","journal-title":"Tissue Eng."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"8414715","DOI":"10.1155\/2016\/8414715","article-title":"Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly-\u03b5-Caprolactone\/\u03b2-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects","volume":"2016","author":"Kim","year":"2016","journal-title":"Stem Cells Int."},{"key":"ref_119","first-page":"1","article-title":"Genes and Proteins Involved in the Regulation of Osteogenesis","volume":"3","author":"Kirkham","year":"2007","journal-title":"Top. Tissue Eng."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jcb.10276","article-title":"Runx2, A Multifunctional Transcription Factor in Skeletal Development","volume":"87","author":"Komori","year":"2002","journal-title":"J. Cell. Biochem."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"e14174","DOI":"10.7717\/peerj.14174","article-title":"Gene expression profiles for in vitro human stem cell differentiation into osteoblasts and osteoclasts: A systematic review","volume":"10","author":"Lim","year":"2022","journal-title":"PeerJ"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s00441-009-0883-x","article-title":"Isolation, characterization and osteogenic differentiation of adipose-derived stem cells: From small to large animal models","volume":"338","author":"Arrigoni","year":"2009","journal-title":"Cell Tissue Res."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.vetimm.2009.06.014","article-title":"Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue","volume":"132","author":"Alves","year":"2009","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"2337","DOI":"10.3727\/096368914X685780","article-title":"Adipose-Derived Stem Cells From Both Visceral and Subcutaneous Fat Deposits Significantly Improve Contractile Function of Infarcted Rat Hearts","volume":"24","author":"Chi","year":"2015","journal-title":"Cell Transplant."},{"key":"ref_125","first-page":"651","article-title":"Phenotypic characteristics of feline adipose-derived stem cells affected by cell passage number","volume":"20","author":"Panasophonkul","year":"2017","journal-title":"Pol. J. Vet. Sci."},{"key":"ref_126","first-page":"638","article-title":"Incidence of Appendicular Bone Fracture in Dogs and Cats: Retrospective Study at Veterinary Hospital of Cairo University and some Private Clinics in Egypt","volume":"10","author":"Ahmed","year":"2020","journal-title":"World\u2019s Vet. J."},{"key":"ref_127","first-page":"127","article-title":"Incidence and classification of bone fracture in dogs and cats: A retrospective study at a Veterinary Teaching Hospital, Khon Kaen University, Thailand (2013\u20132016)","volume":"17","author":"Keosengthong","year":"2019","journal-title":"Vet. Integr. Sci."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1002\/term.2806","article-title":"Conditioned media derived from mesenchymal stem cell cultures: The next generation for regenerative medicine","volume":"13","author":"Gunawardena","year":"2019","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_129","doi-asserted-by":"crossref","unstructured":"Mahiddine, F.Y., Kim, J.W., Qamar, A.Y., Ra, J.C., Kim, S.H., Jung, E.J., and Kim, M.J. (2020). Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters. Animals, 10.","DOI":"10.3390\/ani10101899"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1089\/ten.tea.2011.0325","article-title":"Conditioned Media from Mesenchymal Stem Cells Enhanced Bone Regeneration in Rat Calvarial Bone Defects","volume":"18","author":"Osugi","year":"2012","journal-title":"Tissue Eng. Part A"},{"key":"ref_131","doi-asserted-by":"crossref","unstructured":"Pawitan, J.A. (2014). Prospect of Stem Cell Conditioned Medium in Regenerative Medicine. BioMed Res. Int., 2014.","DOI":"10.1155\/2014\/965849"},{"key":"ref_132","doi-asserted-by":"crossref","unstructured":"Vizoso, F.J., Eiro, N., Cid, S., Schneider, J., and Perez-Fernandez, R. (2017). Mesenchymal Stem Cell Secretome: Toward Cell-Free Therapeutic Strategies in Regenerative Medicine. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18091852"},{"key":"ref_133","doi-asserted-by":"crossref","unstructured":"Linkova, D.D., Rubtsova, Y.P., and Egorikhina, M.N. (2022). Cryostorage of Mesenchymal Stem Cells and Biomedical Cell-Based Products. Cells, 11.","DOI":"10.3390\/cells11172691"},{"key":"ref_134","doi-asserted-by":"crossref","unstructured":"Mocchi, M., Dotti, S., Del Bue, M., Villa, R., Bari, E., Perteghella, S., Torre, M.L., and Grolli, S. (2020). Veterinary Regenerative Medicine for Musculoskeletal Disorders: Can Mesenchymal Stem\/Stromal Cells and Their Secretome Be the New Frontier?. Cells, 9.","DOI":"10.3390\/cells9061453"},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1089\/ten.tec.2009.0512","article-title":"Cryopreservation does not affect the stem characteristics of multipotent cells isolated from equine peripheral blood","volume":"16","author":"Martinello","year":"2010","journal-title":"Tissue Eng. Part C Methods"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/12\/673\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:29:34Z","timestamp":1760131774000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/12\/673"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,24]]},"references-count":135,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["vetsci10120673"],"URL":"https:\/\/doi.org\/10.3390\/vetsci10120673","relation":{},"ISSN":["2306-7381"],"issn-type":[{"type":"electronic","value":"2306-7381"}],"subject":[],"published":{"date-parts":[[2023,11,24]]}}}