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A. Heleno and the individual scientific employment contract of F. S. Reis","award":["UI\/00772"],"award-info":[{"award-number":["UI\/00772"]}]},{"name":"institutional contract awarded to S. A. Heleno and the individual scientific employment contract of F. S. Reis","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}]},{"name":"institutional contract awarded to S. A. Heleno and the individual scientific employment contract of F. S. Reis","award":["UIDB\/00690\/2020"],"award-info":[{"award-number":["UIDB\/00690\/2020"]}]},{"name":"institutional contract awarded to S. A. Heleno and the individual scientific employment contract of F. S. Reis","award":["UIDP\/00690\/2020"],"award-info":[{"award-number":["UIDP\/00690\/2020"]}]},{"name":"institutional contract awarded to S. A. Heleno and the individual scientific employment contract of F. S. Reis","award":["LA\/P\/0007\/2020"],"award-info":[{"award-number":["LA\/P\/0007\/2020"]}]},{"name":"institutional contract awarded to S. A. Heleno and the individual scientific employment contract of F. S. Reis","award":["2023.00592.BD"],"award-info":[{"award-number":["2023.00592.BD"]}]},{"name":"institutional contract awarded to S. A. Heleno and the individual scientific employment contract of F. S. Reis","award":["2021.03728.CEECIND"],"award-info":[{"award-number":["2021.03728.CEECIND"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Sternal bursitis is an underexplored lesion in poultry, often overlooked in microbiological diagnostics. In this study, we characterized 36 Escherichia coli isolates recovered from sternal bursitis in broiler chickens, combining phenotypic antimicrobial susceptibility testing, PCR-based screening, and whole genome sequencing (WGS). The genetic analysis revealed a diverse population spanning 15 sequence types, including ST155, ST201, and ST58. Resistance to tetracycline and ciprofloxacin was common, and several isolates carried genes encoding \u03b2-lactamases, including blaTEM-1B. Chromosomal mutations associated with quinolone and fosfomycin resistance (e.g., gyrA p.S83L, glpT_E448K) were also identified. WGS revealed a high number of virulence-associated genes per isolate (58\u201396), notably those linked to adhesion (fim, ecp clusters), secretion systems (T6SS), and iron acquisition (ent, fep, fes), suggesting strong pathogenic potential. Many isolates harbored virulence markers typical of ExPEC\/APEC, such as iss, ompT, and traT, even in the absence of multidrug resistance. Our findings suggest that E. coli from sternal bursitis may act as reservoirs of resistance and virulence traits relevant to animal and public health. This highlights the need for including such lesions in genomic surveillance programs and reinforces the importance of integrated One Health approaches.<\/jats:p>","DOI":"10.3390\/vetsci12070675","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T14:13:43Z","timestamp":1752761623000},"page":"675","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genomic Analysis of Antibiotic Resistance and Virulence Profiles in Escherichia coli Linked to Sternal Bursitis in Chickens: A One Health Perspective"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8274-2013","authenticated-orcid":false,"given":"Jessica","family":"Ribeiro","sequence":"first","affiliation":[{"name":"MicroART\u2014Microbiology and Antibiotic Resistance Team, Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry, Department of Chemistry, University NOVA of Lisbon, 2829-516 Lisbon, Portugal"},{"name":"CIMO\u2014Centro de Investiga\u00e7\u00e3o de Montanha, La SusTEC, Instituto Polit\u00e9cnico de Bragan\u00e7a, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Functional Genomics and Proteomics Unit, Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9406-8433","authenticated-orcid":false,"given":"Vanessa","family":"Silva","sequence":"additional","affiliation":[{"name":"MicroART\u2014Microbiology and Antibiotic Resistance Team, Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry, Department of Chemistry, University NOVA of Lisbon, 2829-516 Lisbon, Portugal"},{"name":"Functional Genomics and Proteomics Unit, Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]},{"given":"Catarina","family":"Freitas","sequence":"additional","affiliation":[{"name":"MicroART\u2014Microbiology and Antibiotic Resistance Team, Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]},{"given":"Pedro","family":"Pinto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8588-7071","authenticated-orcid":false,"given":"Madalena","family":"Vieira-Pinto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"AL4AnimalS\u2014Associate Laboratory for Animal and Veterinary Science, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"CECAV\u2014Veterinary and Animal Research Centre, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2081-682X","authenticated-orcid":false,"given":"Rita","family":"Batista","sequence":"additional","affiliation":[{"name":"Food Microbiology Laboratory, Food and Nutrition Department, INSA, Avenida Padre Cruz, 1649-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6449-8053","authenticated-orcid":false,"given":"Alexandra","family":"Nunes","sequence":"additional","affiliation":[{"name":"CECAV\u2014Veterinary and Animal Research Centre, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Genomics and Bioinformatics Unit, Department of Infectious Diseases, INSA, 1649-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2697-2399","authenticated-orcid":false,"given":"Jo\u00e3o Paulo","family":"Gomes","sequence":"additional","affiliation":[{"name":"CECAV\u2014Veterinary and Animal Research Centre, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Genomics and Bioinformatics Unit, Department of Infectious Diseases, INSA, 1649-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1963-441X","authenticated-orcid":false,"given":"Jos\u00e9 Eduardo","family":"Pereira","sequence":"additional","affiliation":[{"name":"AL4AnimalS\u2014Associate Laboratory for Animal and Veterinary Science, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"CECAV\u2014Veterinary and Animal Research Centre, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6365-0735","authenticated-orcid":false,"given":"Gilberto","family":"Igrejas","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry, Department of Chemistry, University NOVA of Lisbon, 2829-516 Lisbon, Portugal"},{"name":"Functional Genomics and Proteomics Unit, Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9050-5189","authenticated-orcid":false,"given":"Lillian","family":"Barros","sequence":"additional","affiliation":[{"name":"CIMO\u2014Centro de Investiga\u00e7\u00e3o de Montanha, La SusTEC, Instituto Polit\u00e9cnico de Bragan\u00e7a, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7224-1098","authenticated-orcid":false,"given":"Sandrina A.","family":"Heleno","sequence":"additional","affiliation":[{"name":"CIMO\u2014Centro de Investiga\u00e7\u00e3o de Montanha, La SusTEC, Instituto Polit\u00e9cnico de Bragan\u00e7a, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9159-0530","authenticated-orcid":false,"given":"Filipa S.","family":"Reis","sequence":"additional","affiliation":[{"name":"CIMO\u2014Centro de Investiga\u00e7\u00e3o de Montanha, La SusTEC, Instituto Polit\u00e9cnico de Bragan\u00e7a, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-689X","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Poeta","sequence":"additional","affiliation":[{"name":"MicroART\u2014Microbiology and Antibiotic Resistance Team, Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry, Department of Chemistry, University NOVA of Lisbon, 2829-516 Lisbon, Portugal"},{"name":"AL4AnimalS\u2014Associate Laboratory for Animal and Veterinary Science, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"CECAV\u2014Veterinary and Animal Research Centre, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1080\/23744235.2023.2261538","article-title":"Mortality and Length of Hospital Stay after Bloodstream Infections Caused by ESBL-Producing Compared to Non-ESBL-Producing E. coli","volume":"56","author":"Handal","year":"2024","journal-title":"Infect. 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