{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T21:04:28Z","timestamp":1769634268949,"version":"3.49.0"},"reference-count":71,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["UID\/276\/2025 (CIISA)"],"award-info":[{"award-number":["UID\/276\/2025 (CIISA)"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["LA\/P\/0059\/2020 (AL4AnimalS)"],"award-info":[{"award-number":["LA\/P\/0059\/2020 (AL4AnimalS)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Understanding antimicrobial resistance (AMR) within a One Health framework requires examining how human\u2013animal\u2013environment interactions shape bacterial populations, and captive wildlife offers a unique context to explore these dynamics. This study aimed to characterise the phenotypic resistance and virulence profiles of Escherichia coli isolated from faecal samples of captive non-domestic felids housed in a wildlife sanctuary in the United Kingdom and evaluate the influence of captive conditions in E. coli traits. A total of 41 faecal samples were collected from 36 animals representing 11 non-domestic felid species, from which it was possible to obtain 108 E. coli isolates identified using IMViC testing. The isolates were characterised regarding their susceptibility to 12 antibiotics by disc diffusion and screened for the phenotypic expression of six virulence factors, including protease, DNase, gelatinase, lecithinase, haemolysins, and biofilm formation. The highest resistance rates were observed for tetracycline (19.4%) and ampicillin (10.2%), while isolates presented complete susceptibility regarding half of the tested antibiotics. Also, 9.3% of the isolates presented a multidrug-resistant profile. Biofilm formation was the only virulence factor expressed by the isolates under study (8.3%). Significant associations were detected between resistance outcomes and levels of human proximity and enclosure type. These findings suggest that captivity-related factors may influence AMR profiles in wild felids and highlight the importance of continued AMR surveillance and appropriate management practices to reduce selective pressures in captive wildlife.<\/jats:p>","DOI":"10.3390\/vetsci13020124","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T08:48:04Z","timestamp":1769590084000},"page":"124","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antimicrobial Resistance in Escherichia coli from Captive Wild Felids: Associations with Host and Management Factors"],"prefix":"10.3390","volume":"13","author":[{"given":"Sofia","family":"Caramujo","sequence":"first","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"AL4AnimalS\u2014Associate Laboratory for Animal and Veterinary Sciences, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8063-174X","authenticated-orcid":false,"given":"Raquel","family":"Abreu","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"AL4AnimalS\u2014Associate Laboratory for Animal and Veterinary Sciences, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3533-6893","authenticated-orcid":false,"given":"Gon\u00e7alo","family":"Pereira","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"AL4AnimalS\u2014Associate Laboratory for Animal and Veterinary Sciences, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5641-4400","authenticated-orcid":false,"given":"Eva","family":"Cunha","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"AL4AnimalS\u2014Associate Laboratory for Animal and Veterinary Sciences, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8671-6285","authenticated-orcid":false,"given":"Lu\u00eds","family":"Tavares","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"AL4AnimalS\u2014Associate Laboratory for Animal and Veterinary Sciences, 1300-477 Lisbon, Portugal"}]},{"given":"Emily","family":"McFarlane","sequence":"additional","affiliation":[{"name":"The Big Cat Sanctuary, Headcorn Rd, Smarden, Ashford TN27 8PJ, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3910-1525","authenticated-orcid":false,"given":"Manuela","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"AL4AnimalS\u2014Associate Laboratory for Animal and Veterinary Sciences, 1300-477 Lisbon, Portugal"},{"name":"cE3c\u2014Centre for Ecology, Evolution and Environmental Changes, CHANGE\u2014Global Change and Sustainability Institute, Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"ref_1","unstructured":"WHO (2025). 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