{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:10:26Z","timestamp":1777630226176,"version":"3.51.4"},"reference-count":56,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T00:00:00Z","timestamp":1590710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Bovine Ocular Squamous Cell Carcinoma is considered the most common bovine tumour, causing significant economic losses, mainly by abattoir condemnations. To obtain a better insight into the genesis and neoplastic transformation, 19 samples collected at slaughter from Holstein Friesian cattle and diagnosed as Ocular Squamous Cell Carcinoma were studied. Tumours were histologically classified into three categories: poorly (26.3%), moderately (26.3%), and well differentiated (47.4%). Expression of keratins (MNF116 and LP34) and of cornified envelope precursors (involucrin and profilaggrin) was studied. Expression of MNF116 was observed in all carcinomas. LP34 immunostaining was seen in all but three carcinomas, one from each degree. Involucrin immunoreaction was observed in all but one poorly differentiated carcinoma. Profilaggrin was present in only two moderately differentiated carcinomas, in all but one well differentiated, and in all but one poorly differentiated. MNF116 is a useful marker to confirm the epithelial origin of the tumour and stain most neoplastic cells in these tumours. The expression of involucrin and LP34 demonstrates that, in all tumours, cells have reached the final program of differentiation, regardless of the grade. The expression of profilaggrin could indicate molecular changes during malignant transformation but their expression does not seem to be of diagnostic value.<\/jats:p>","DOI":"10.3390\/vetsci7020070","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T11:49:21Z","timestamp":1591012161000},"page":"70","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Immunohistochemical Studies of Cytokeratins and Differentiation Markers in Bovine Ocular Squamous Cell Carcinoma"],"prefix":"10.3390","volume":"7","author":[{"given":"Helena","family":"Vala","sequence":"first","affiliation":[{"name":"Agrarian School of the Polytechnic Institute of Viseu, Quinta da Alagoa-Estrada de Nelas Ranhados, 3500-606 Viseu, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal"}]},{"given":"T\u00e2nia","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Av. Professor Egas Moniz, 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5908-4726","authenticated-orcid":false,"given":"Carlos","family":"Pinto","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e do Ambiente\u2014Universidade dos A\u00e7ores, Rua Capit\u00e3o Jo\u00e3o d\u2019\u00c1vila\u2014Pico da Urze, 9700-042 Angra do Hero\u00edsmo, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4054-9124","authenticated-orcid":false,"given":"Maria A.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Agrarian School of the Polytechnic Institute of Viseu, Quinta da Alagoa-Estrada de Nelas Ranhados, 3500-606 Viseu, Portugal"},{"name":"Global Health and Tropical Medicine (GHTM), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), R. da Junqueira 100, 1349-008 Lisboa, Portugal"}]},{"given":"Jo\u00e3o R.","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"given":"Maria C.","family":"Peleteiro","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, Avenida da Universidade Te\u0301cnica, 1300-477 Lisboa, Portugal"}]},{"given":"Llu\u00eds","family":"Ferrer","sequence":"additional","affiliation":[{"name":"Departament de Medicina i Cirurgia Animals, Facultat de Veterin\u00e0ria, Universitat Aut\u00f2noma de Barcelona, 08193 Barcelona, Spain"}]},{"given":"Dolores","family":"Fondevila","sequence":"additional","affiliation":[{"name":"Departament de Medicina i Cirurgia Animals, Facultat de Veterin\u00e0ria, Universitat Aut\u00f2noma de Barcelona, 08193 Barcelona, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.14202\/vetworld.2017.1413-1420","article-title":"Ocular squamous cell carcinoma in Holstein cows from the South of Brazil","volume":"10","author":"Fornazari","year":"2017","journal-title":"Vet. 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