{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T11:25:33Z","timestamp":1764588333149,"version":"build-2065373602"},"reference-count":119,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["UIDB\/04308\/2020","UID\/IC\/00051\/2013"],"award-info":[{"award-number":["UIDB\/04308\/2020","UID\/IC\/00051\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Over the last years, the importance of microRNAs (miRNAs) has increasingly been recognised. Each miRNA is a short sequence of non-coding RNA that influences countless genes\u2019 expression and, thereby, contributes to several physiological pathways and diseases. It has been demonstrated that miRNAs participate in the development of many cardiovascular diseases (CVDs). This review synopsises the most recent studies emphasising miRNA\u2019s influence in several CVDs affecting dogs and cats. It provides a concise outline of miRNA\u2019s biology and function, the diagnostic potential of circulating miRNAs as biomarkers, and their role in different CVDs. It also discusses known and future roles for miRNAs as potential clinical biomarkers and therapeutic targets. So, this review gives a comprehensive outline of the most relevant miRNAs related to CVDs in Veterinary Medicine.<\/jats:p>","DOI":"10.3390\/vetsci9100533","type":"journal-article","created":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T01:23:16Z","timestamp":1664414596000},"page":"533","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Emerging Roles of Micrornas in Veterinary Cardiology"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5397-6216","authenticated-orcid":false,"given":"Ana","family":"Reis-Ferreira","sequence":"first","affiliation":[{"name":"Hospital Veterin\u00e1rio do Porto, Travessa Silva Porto 174, 4250-475 Porto, Portugal"},{"name":"ICBAS-UP, Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8929-9253","authenticated-orcid":false,"given":"Joana","family":"Neto-Mendes","sequence":"additional","affiliation":[{"name":"ICBAS-UP, Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1527-3776","authenticated-orcid":false,"given":"Carmen","family":"Br\u00e1s-Silva","sequence":"additional","affiliation":[{"name":"UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"}]},{"given":"Lu\u00eds","family":"Lobo","sequence":"additional","affiliation":[{"name":"Hospital Veterin\u00e1rio do Porto, Travessa Silva Porto 174, 4250-475 Porto, Portugal"},{"name":"Faculdade de Medicina Veterin\u00e1ria, Universidade Lus\u00f3fona de Humanidades e Tecnologias, Campo Grande 376, 1749-024 Lisboa, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal, Campus Agr\u00e1rio de Vair\u00e3o, 4480-009 Vila do Conde, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3873-3886","authenticated-orcid":false,"given":"Ana Patr\u00edcia","family":"Fontes-Sousa","sequence":"additional","affiliation":[{"name":"ICBAS-UP, Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"Departamento de Imuno-Fisiologia e Farmacologia, Centro de Investiga\u00e7\u00e3o Farmacol\u00f3gica e Inova\u00e7\u00e3o Medicamentosa (MedInUP), Universidade do Porto, Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"UPVET, Hospital Veterin\u00e1rio da Universidade do Porto, Rua Jorge de Viterbo Ferreira 132, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/nrg2521","article-title":"Long non-coding RNAs: Insights into functions","volume":"10","author":"Mercer","year":"2009","journal-title":"Nat. Rev. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1146\/annurev.genom.8.080706.092351","article-title":"MicroRNAs in vertebrate physiology and human disease","volume":"8","author":"Chang","year":"2007","journal-title":"Annu. Rev. Genom. Hum. Genet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/0092-8674(93)90529-Y","article-title":"The C. elegans Heterochronic Gene lin-4 Encodes Small RNAs with Antisense Complementarity to lin-14","volume":"75","author":"Lee","year":"1993","journal-title":"Cell"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/nrg2843","article-title":"The widespread regulation of microRNA biogenesis, function and decay","volume":"11","author":"Krol","year":"2010","journal-title":"Nat. Rev. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/j.bbamcr.2010.06.013","article-title":"MicroRNAs: Synthesis, mechanism, function, and recent clinical trials","volume":"1803","author":"Wahid","year":"2010","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/s00059-013-4047-0","article-title":"MicroRNA-based therapy in cardiology","volume":"39","author":"Fiedler","year":"2014","journal-title":"Herz"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1373\/clinchem.2010.147405","article-title":"The MicroRNA Spectrum in 12 Body Fluids","volume":"56","author":"Weber","year":"2010","journal-title":"Clin. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.pharmthera.2012.08.003","article-title":"Cell-to-cell miRNA transfer: From body homeostasis to therapy","volume":"136","author":"Redis","year":"2012","journal-title":"Pharmacol. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.3389\/fphar.2019.01500","article-title":"Non-exosomal and exosomal circulatory MicroRNAs: Which are more valid as biomarkers?","volume":"10","author":"Kamal","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1533033818821421","DOI":"10.1177\/1533033818821421","article-title":"Exosomes: A Promising Avenue for the Diagnosis of Breast Cancer","volume":"18","author":"Meng","year":"2019","journal-title":"Technol. Cancer Res. Treat"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1186\/s12964-020-00650-6","article-title":"Exosomal microRNAs derived from mesenchymal stem cells: Cell-to-cell messages","volume":"18","author":"Asgarpour","year":"2020","journal-title":"Cell Commun. Signal"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"372","DOI":"10.21037\/atm-22-619","article-title":"Potential clinical applications of exosomes in the diagnosis, treatment, and prognosis of cardiovascular diseases: A narrative review","volume":"10","author":"Chen","year":"2022","journal-title":"Ann. Transl. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"10513","DOI":"10.1073\/pnas.0804549105","article-title":"Circulating microRNAs as stable blood-based markers for cancer detection","volume":"105","author":"Mitchell","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/s41374-018-0143-3","article-title":"MicroRNA amplification and detection technologies: Opportunities and challenges for point of care diagnostics","volume":"99","author":"Dave","year":"2019","journal-title":"Lab. Investig."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1038\/ni.f.209","article-title":"MicroRNAs: New regulators of immune cell development and function","volume":"9","author":"Baltimore","year":"2008","journal-title":"Nat. Immunol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Islas, J.F., and Moreno-Cuevas, J.E. (2018). A MicroRNA perspective on cardiovascular development and diseases: An update. Int. J. Mol. Sci., 19.","DOI":"10.20944\/preprints201805.0154.v1"},{"key":"ref_17","first-page":"60","article-title":"MicroRNA and Cardiovascular Diseases","volume":"37","author":"Demir","year":"2020","journal-title":"Balk. Med. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2365","DOI":"10.4103\/0366-6999.190677","article-title":"Regulatory Mechanisms of the Molecular Pathways in Fibrosis Induced by MicroRNAs","volume":"129","author":"Yang","year":"2016","journal-title":"Chin. Med. J."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wehbe, N., Nasser, S.A., Pintus, G., Badran, A., Eid, A.H., and Baydoun, E. (2019). MicroRNAs in Cardiac Hypertrophy. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20194714"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.1016\/j.bbadis.2016.11.034","article-title":"The role of microRNAs in heart failure","volume":"1863","author":"Wang","year":"2017","journal-title":"Biochim. Biophys. Acta (BBA) Mol. Basis Dis."},{"key":"ref_21","first-page":"985408","article-title":"MicroRNAs Expression Profiles in Cardiovascular Diseases","volume":"2014","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"R169","DOI":"10.1093\/hmg\/ddq367","article-title":"Analysis of microRNA knockouts in mice","volume":"19","author":"Park","year":"2010","journal-title":"Hum. Mol. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1161\/CIRCRESAHA.109.200451","article-title":"Loss of cardiac microRNA-mediated regulation leads to dilated cardiomyopathy and heart failure","volume":"105","author":"Rao","year":"2009","journal-title":"Circ. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.stem.2008.01.016","article-title":"MicroRNA Regulation of Cell Lineages in Mouse and Human Embryonic Stem Cells","volume":"2","author":"Ivey","year":"2008","journal-title":"Cell Stem Cell"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.devcel.2009.10.013","article-title":"A Family of microRNAs Encoded by Myosin Genes Governs Myosin Expression and Muscle Performance","volume":"17","author":"Quiat","year":"2009","journal-title":"Dev. Cell"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1161\/CIRCRESAHA.111.248880","article-title":"MiR-15 family regulates postnatal mitotic arrest of cardiomyocytes","volume":"109","author":"Porrello","year":"2011","journal-title":"Circ. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.cell.2008.02.019","article-title":"Targeted Deletion Reveals Essential and Overlapping Functions of the miR-17\u223c92 Family of miRNA Clusters","volume":"132","author":"Ventura","year":"2008","journal-title":"Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2370","DOI":"10.1001\/jama.285.18.2370","article-title":"Prevalence of diagnosed atrial fibrillation in adults: National implications for rhythm management and stroke prevention: The anticoagulation and risk factors in atrial fibrillation (ATRIA) study","volume":"285","author":"Go","year":"2001","journal-title":"J. Am. Med. Assoc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1515\/jvetres-2017-0013","article-title":"Prevalence of arrhythmias in dogs examined between 2008 and 2014","volume":"61","author":"Cepiel","year":"2017","journal-title":"J. Vet. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"105548","DOI":"10.1016\/j.tvjl.2020.105548","article-title":"Canine atrial fibrillation: Pathophysiology, epidemiology and classification","volume":"265","author":"Pedro","year":"2020","journal-title":"Vet. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.jjcc.2019.05.018","article-title":"MicroRNAs: Emerging biomarkers for atrial fibrillation","volume":"74","author":"Komal","year":"2019","journal-title":"J. Cardiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.21037\/atm-21-4483","article-title":"A narrative review of non-coding RNAs in atrial fibrillation: Potential therapeutic targets and molecular mechanisms","volume":"9","author":"Zhang","year":"2021","journal-title":"Ann. Transl. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1161\/CIRCULATIONAHA.112.001207","article-title":"MicroRNA29: A mechanistic contributor and potential biomarker in atrial fibrillation","volume":"127","author":"Dawson","year":"2013","journal-title":"Circulation"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.yjmcc.2014.10.001","article-title":"Detailed characterization of microRNA changes in a canine heart failure model: Relationship to arrhythmogenic structural remodeling","volume":"77","author":"Chen","year":"2014","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_35","first-page":"1457","article-title":"Expression of miR-133 and miR-30 in chronic atrial fibrillation in canines","volume":"5","author":"Li","year":"2012","journal-title":"Mol. Med. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4143","DOI":"10.3892\/mmr.2017.7045","article-title":"miR-132 in atrial fibrillation directly targets connective tissue growth factor","volume":"16","author":"Qiao","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1161\/01.CIR.103.1.22","article-title":"Nerve Sprouting and Sympathetic Hyperinnervation in a Canine Model of Atrial Fibrillation Produced by Prolonged Right Atrial Pacing","volume":"103","author":"Chang","year":"2001","journal-title":"Circulation"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Zheng, S., Geng, Y., Xue, J., and Wang, Z. (2015). MicroRNA Profiling of Atrial Fibrillation in Canines: MiR-206 Modulates Intrinsic Cardiac Autonomic Nerve Remodeling by Regulating SOD1. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0122674"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"108004","DOI":"10.1016\/j.diabres.2020.108004","article-title":"The functions of microRNA-208 in the heart","volume":"160","author":"Zhao","year":"2020","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"359","DOI":"10.2478\/jvetres-2018-0051","article-title":"Screening for circulating miR-208a and-b in different cardiac arrhythmias of dogs","volume":"62","author":"Zacharski","year":"2018","journal-title":"J. Vet. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.jvc.2012.01.011","article-title":"Historical review, epidemiology and natural history of degenerative mitral valve disease","volume":"14","author":"Borgarelli","year":"2012","journal-title":"J. Vet. Cardiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.jvc.2012.01.005","article-title":"Pathology, protein expression and signaling in myxomatous mitral valve degeneration: Comparison of dogs and humans","volume":"14","author":"Aupperle","year":"2012","journal-title":"J. Vet. Cardiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1111\/jvim.15488","article-title":"ACVIM consensus guidelines for the diagnosis and treatment of myxomatous mitral valve disease in dogs","volume":"33","author":"Keene","year":"2019","journal-title":"J. Vet. Intern. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jvc.2021.04.005","article-title":"The Longitudinal Outcome Of Canine (K9) myxomatous mitral valve disease (LOOK-Mitral registry): Baseline characteristics","volume":"36","author":"Franchini","year":"2021","journal-title":"J. Vet. Cardiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1350088","DOI":"10.1080\/20013078.2017.1350088","article-title":"Circulating exosome microRNA associated with heart failure secondary to myxomatous mitral valve disease in a naturally occurring canine model","volume":"6","author":"Yang","year":"2017","journal-title":"J. Extracell. Vesicles"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Yang, V.K., Tai, A.K., Huh, T.P., Meola, D.M., Juhr, C.M., Robinson, N.A., and Hoffman, A.M. (2018). Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0188617"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.tvjl.2015.02.021","article-title":"Gene network and canonical pathway analysis in canine myxomatous mitral valve disease: A microarray study","volume":"204","author":"Lu","year":"2015","journal-title":"Vet. J."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Hulanicka, M., Garncarz, M., Parzeniecka-Jaworska, M., and Jank, M. (2014). Plasma miRNAs as potential biomarkers of chronic degenerative valvular disease in Dachshunds. BMC Vet. Res., 10.","DOI":"10.1186\/s12917-014-0205-8"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1161\/CIRCRESAHA.108.182535","article-title":"miR-133 and miR-30 regulate connective tissue growth factor: Implications for a role of microRNAs in myocardial matrix remodeling","volume":"104","author":"Duisters","year":"2009","journal-title":"Circ. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"14098","DOI":"10.3390\/ijms160614098","article-title":"Expression Profiling of Circulating MicroRNAs in Canine Myxomatous Mitral Valve Disease","volume":"16","author":"Li","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"729929","DOI":"10.3389\/fvets.2021.729929","article-title":"Identification and Characterization of Circulating MicroRNAs as Novel Biomarkers in Dogs With Heart Diseases","volume":"8","author":"Ro","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_52","first-page":"463","article-title":"Matrix Metalloproteinases (MMPs), Their Genetic Variants and miRNA in Mitral Valve Diseases: Potential Biomarker Tools and Targets for Personalized Treatments","volume":"25","author":"Balistreri","year":"2016","journal-title":"J. Heart Valve Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e523","DOI":"10.1016\/j.jacc.2008.05.007","article-title":"2008 Focused update incorporated into the ACC\/AHA 2006 guidelines for the management of patients with valvular heart disease","volume":"52","author":"Bonow","year":"2008","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Songia, P., Chiesa, M., Alfieri, V., Massaiu, I., Moschetta, D., Myasoedova, V., Valerio, V., Fusini, L., Gripari, P., and Zanobini, M. (2021). Putative Circulating MicroRNAs Are Able to Identify Patients with Mitral Valve Prolapse and Severe Regurgitation. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22042102"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"652224","DOI":"10.3389\/fvets.2021.652224","article-title":"Expression Profile of Circulating MicroRNAs in Dogs With Cardiac Hypertrophy: A Pilot Study","volume":"8","author":"Ro","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/jsap.12841","article-title":"An update on canine cardiomyopathies\u2014Is it all in the genes?","volume":"59","author":"Dutton","year":"2018","journal-title":"J. Small Anim. Pract."},{"key":"ref_57","unstructured":"Ettinger, S.J., Feldman, E.C., and Cote, E. (2017). Textbook of Veterinary Internal Medicine, Elsevier. [8th ed.]."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Steudemann, C., Bauersachs, S., Weber, K., and Wess, G. (2013). Detection and comparison of microRNA expression in the serum of Doberman Pinschers with dilated cardiomyopathy and healthy controls. BMC Vet. Res., 9.","DOI":"10.1186\/1746-6148-9-12"},{"key":"ref_59","first-page":"86","article-title":"Emerging role of microRNAs in dilated cardiomyopathy: Evidence regarding etiology","volume":"215","author":"Belmonte","year":"2019","journal-title":"Transl. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"285","DOI":"10.17219\/acem\/115088","article-title":"Relations between circulating and myocardial fibrosis-linked microRNAs with left ventricular reverse remodeling in dilated cardiomyopathy","volume":"29","author":"Pitera","year":"2020","journal-title":"Adv. Clin. Exp. Med."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Chiti, E., Paolo, M.D., Turillazzi, E., and Rocchi, A. (2021). MicroRNAs in Hypertrophic, Arrhythmogenic and Dilated Cardiomyopathy. Diagnostics, 11.","DOI":"10.3390\/diagnostics11091720"},{"key":"ref_62","first-page":"205","article-title":"Diagnostic methods for the canine idiopathic dilated cardiomyopathy: A narrative evidence-based rapid review","volume":"128","author":"Alessi","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1111\/jvim.15745","article-title":"ACVIM consensus statement guidelines for the classification, diagnosis, and management of cardiomyopathies in cats","volume":"34","author":"Abbott","year":"2020","journal-title":"J. Vet. Intern. Med."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s11010-014-2324-8","article-title":"Serum microRNA profiles in cats with hypertrophic cardiomyopathy","volume":"402","author":"Weber","year":"2015","journal-title":"Mol. Cell. Biochem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.jacc.2021.12.002","article-title":"Diagnosis and Evaluation of Hypertrophic Cardiomyopathy: JACC State-of-the-Art Review","volume":"79","author":"Maron","year":"2022","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2266","DOI":"10.1111\/jcmm.12380","article-title":"MiR-451 is decreased in hypertrophic cardiomyopathy and regulates autophagy by targeting TSC1","volume":"18","author":"Song","year":"2014","journal-title":"J. Cell Mol. Med."},{"key":"ref_67","first-page":"983290","article-title":"MicroRNAs Based Therapy of Hypertrophic Cardiomyopathy: The Road Traveled so Far","volume":"2015","author":"Raposo","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"109131","DOI":"10.1016\/j.vetpar.2020.109131","article-title":"Treatment of dogs with severe heartworm disease","volume":"283","author":"Ames","year":"2020","journal-title":"Vet. Parasitol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0065-308X(08)00204-2","article-title":"Heartworm disease in animals and humans","volume":"66","author":"McCall","year":"2008","journal-title":"Adv. Parasitol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"S190","DOI":"10.1016\/j.jvc.2015.08.004","article-title":"Feline heartworm disease: A\u2019Rubik\u2019s-cube-like\u2019 diagnostic and therapeutic challenge","volume":"17","author":"Venco","year":"2015","journal-title":"J. Vet. Cardiol."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Fuehrer, H.P., Morelli, S., Unterk\u00f6fler, M.S., Bajer, A., Bakran-Lebl, K., Dwu\u017cnik-Szarek, D., Farkas, R., Grandi, G., Heddergott, M., and Jokelainen, P. (2021). Dirofilaria spp. and Angiostrongylus vasorum: Current Risk of Spreading in Central and Northern Europe. Pathogens, 10.","DOI":"10.3390\/pathogens10101268"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Tritten, L., Burkman, E., Moorhead, A., Satti, M., Geary, J., Mackenzie, C., and Geary, T. (2014). Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002971"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1645\/18-12","article-title":"Further Characterization of Molecular Markers in Canine Dirofilaria immitis Infection","volume":"104","author":"Braman","year":"2018","journal-title":"J. Parasitol."},{"key":"ref_74","first-page":"234","article-title":"Subcutaneous Human Dirofilariosis By D. Repens In South Italy: A Case Report","volume":"42","author":"Brindicci","year":"2019","journal-title":"New Microbiol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1007\/s00436-017-5382-5","article-title":"Plasma-derived parasitic microRNAs have insufficient concentrations to be used as diagnostic biomarker for detection of Onchocerca volvulus infection or treatment monitoring using LNA-based RT-qPCR","volume":"116","author":"Lagatie","year":"2017","journal-title":"Parasitol. Res."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Ricciardi, A., Bennuru, S., Tariq, S., Kaur, S., Wu, W., Elkahloun, A.G., Arakelyan, A., Shaik, J., Dorward, D.W., and Nutman, T.B. (2021). Extracellular vesicles released from the filarial parasite Brugia malayi downregulate the host mTOR pathway. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0008884"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1093\/eurheartj\/ehw128","article-title":"2016 ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure: The Task Force for the diagnosis and treatment of acute and chronic heart failure of the European Society of Cardiology (ESC)Developed with the special contribution of the Heart Failure Association (HFA) of the ESC","volume":"37","author":"Ponikowski","year":"2016","journal-title":"Eur. Heart J."},{"key":"ref_78","unstructured":"Dukes, H.H. (2015). Dukes\u2019 Physiology of Domestic Animals, Wiley-Blackwell. [13th ed.]."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1111\/joim.12756","article-title":"Biomarkers for heart failure: Small molecules with high clinical relevance","volume":"283","author":"Magnussen","year":"2018","journal-title":"J. Intern. Med."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"163","DOI":"10.2460\/ajvr.79.2.163","article-title":"Genome-wide sequencing and quantification of circulating microRNAs for dogs with congestive heart failure secondary to myxomatous mitral valve degeneration","volume":"79","author":"Jung","year":"2018","journal-title":"Am. J. Vet. Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1002\/ejhf.495","article-title":"MicroRNAs in heart failure: From biomarker to target for therapy","volume":"18","author":"Vegter","year":"2016","journal-title":"Eur. J. Heart Fail."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"199","DOI":"10.5326\/15473317-36-3-199","article-title":"Clinical findings and coronary artery disease in dogs and cats with acute and subacute myocardial necrosis: 28 cases","volume":"36","author":"Kidd","year":"2000","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_83","unstructured":"Nelson, R., and Couto, C.G. (2019). Small Animal Internal Medicine, Elsevier. [6th ed.]."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.1748-5827.2000.tb03173.x","article-title":"Ischaemic heart disease in the dog: A review of 65 cases","volume":"41","author":"Falk","year":"2000","journal-title":"J. Small Anim. Pr."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.atherosclerosis.2017.12.022","article-title":"Genetic polymorphisms offer insight into the causal role of microRNA in coronary artery disease","volume":"269","author":"Borghini","year":"2018","journal-title":"Atherosclerosis"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Fazmin, I.T., Achercouk, Z., Edling, C.E., Said, A., and Jeevaratnam, K. (2020). Circulating microrna as a biomarker for coronary artery disease. Biomolecules, 10.","DOI":"10.3390\/biom10101354"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1038\/aps.2017.205","article-title":"Non-coding RNAs as biomarkers for acute myocardial infarction review-article","volume":"39","author":"Wang","year":"2018","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1111\/jvim.15762","article-title":"Extracellular vesicular microRNAs as potential biomarker forearly detection of doxorubicin-induced cardiotoxicity","volume":"34","author":"Beaumier","year":"2020","journal-title":"J. Vet. Intern. Med."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"H1443","DOI":"10.1152\/ajpheart.00252.2018","article-title":"Identification of a novel microRNA profile in pediatric patients with cancer treated with anthracycline chemotherapy","volume":"315","author":"Oatmen","year":"2018","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_90","first-page":"3600","article-title":"MiR-378 modulates energy imbalance and apoptosis of mitochondria induced by doxorubicin","volume":"10","author":"Wang","year":"2018","journal-title":"Am. J. Transl. Res."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.redox.2017.12.013","article-title":"MicroRNA-140-5p aggravates doxorubicin-induced cardiotoxicity by promoting myocardial oxidative stress via targeting Nrf2 and Sirt2","volume":"15","author":"Zhao","year":"2018","journal-title":"Redox Biol."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Pellegrini, L., Sileno, S., D\u2019agostino, M., Foglio, E., Florio, M.C., Guzzanti, V., Russo, M.A., Limana, F., and Magenta, A. (2020). MicroRNAs in cancer treatment-induced cardiotoxicity. Cancers, 12.","DOI":"10.3390\/cancers12030704"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1038\/embor.2010.117","article-title":"The ZEB\/miR-200 feedback loop\u2014A motor of cellular plasticity in development and cancer?","volume":"11","author":"Brabletz","year":"2010","journal-title":"EMBO Rep."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"e3020","DOI":"10.1038\/cddis.2017.409","article-title":"Doxorubicin upregulates CXCR4 via miR-200c\/ZEB1-dependent mechanism in human cardiac mesenchymal progenitor cells","volume":"8","author":"Beji","year":"2017","journal-title":"Cell Death Dis."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1038\/cdd.2011.42","article-title":"miR-200c is upregulated by oxidative stress and induces endothelial cell apoptosis and senescence via ZEB1 inhibition","volume":"18","author":"Magenta","year":"2011","journal-title":"Cell Death Differ."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"11879","DOI":"10.1038\/s41598-017-12192-y","article-title":"Activation of miR-34a-5p\/Sirt1\/p66shc pathway contributes to doxorubicin-induced cardiotoxicity","volume":"7","author":"Zhu","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"e004653","DOI":"10.1161\/JAHA.116.004653","article-title":"Circulating microRNAs: Potential Markers of Cardiotoxicity in Children and Young Adults Treated With Anthracycline Chemotherapy","volume":"6","author":"Leger","year":"2017","journal-title":"J. Am. Heart Assoc."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"13027","DOI":"10.1073\/pnas.0805038105","article-title":"Dysregulation of microRNAs after myocardial infarction reveals a role of miR-29 in cardiac fibrosis","volume":"105","author":"Sutherland","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"e1754","DOI":"10.1038\/cddis.2015.89","article-title":"Myocardial MiR-30 downregulation triggered by doxorubicin drives alterations in \u03b2-adrenergic signaling and enhances apoptosis","volume":"6","author":"Castellano","year":"2015","journal-title":"Cell Death Dis."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1097\/HCO.0000000000000615","article-title":"Noncoding RNAs in cardiovascular diseases","volume":"34","author":"Gurha","year":"2019","journal-title":"Curr. Opin. Cardiol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1093\/eurheartj\/ehy339","article-title":"2018 ESC\/ESH Guidelines for the management of arterial hypertension: The Task Force for the management of arterial hypertension of the European Society of Cardiology (ESC) and the European Society of Hypertension (ESH)","volume":"39","author":"Williams","year":"2018","journal-title":"Eur. Heart J."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1111\/jvim.15331","article-title":"ACVIM consensus statement: Guidelines for the identification, evaluation, and management of systemic hypertension in dogs and cats","volume":"32","author":"Acierno","year":"2018","journal-title":"J. Vet. Intern. Med."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.biopha.2017.07.091","article-title":"Renin-angiotensin-aldosterone (RAAS): The ubiquitous system for homeostasis and pathologies","volume":"94","author":"Patel","year":"2017","journal-title":"Biomed. Pharm."},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Improta-Caria, A.C., Aras, M.G., Nascimento, L., De Sousa, R.A.L., Aras-J\u00fanior, R., and Souza, B.S.F. (2021). MicroRNAs Regulating Renin-Angiotensin-Aldosterone System, Sympathetic Nervous System and Left Ventricular Hypertrophy in Systemic Arterial Hypertension. Biomolecules, 11.","DOI":"10.3390\/biom11121771"},{"key":"ref_105","first-page":"11761","article-title":"Correlation between serum microRNA-136 levels and RAAS biochemical markers in patients with essential hypertension","volume":"24","author":"Chu","year":"2020","journal-title":"Eur. Rev. Med. Pharm. Sci."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"BSR20200378","DOI":"10.1042\/BSR20200378","article-title":"Blood microRNA 202-3p associates with the risk of essential hypertension by targeting soluble ST2","volume":"40","author":"Li","year":"2020","journal-title":"Biosci. Rep."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1161\/HYPERTENSIONAHA.111.180729","article-title":"Gene expression profiling reveals renin mRNA overexpression in human hypertensive kidneys and a role for microRNAs","volume":"58","author":"Marques","year":"2011","journal-title":"Hypertension"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.yexcr.2018.02.029","article-title":"Alteration in microRNA-25 expression regulate cardiac function via renin secretion","volume":"365","author":"Li","year":"2018","journal-title":"Exp. Cell Res."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"11190","DOI":"10.3390\/ijms140611190","article-title":"Angiotensin II regulates microRNA-132\/-212 in hypertensive rats and humans","volume":"14","author":"Eskildsen","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"703860","DOI":"10.3389\/fgene.2021.703860","article-title":"Non-Coding RNA Networks in Pulmonary Hypertension","volume":"12","author":"Zang","year":"2021","journal-title":"Front. Genet."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1164\/rccm.201205-0839OC","article-title":"Reduced microRNA-150 is associated with poor survival in pulmonary arterial hypertension","volume":"187","author":"Rhodes","year":"2013","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1164\/rccm.201308-1403LE","article-title":"miR-26a linked to pulmonary hypertension by global assessment of circulating extracellular microRNAs","volume":"188","author":"Schlosser","year":"2013","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_113","doi-asserted-by":"crossref","unstructured":"Wei, C., Henderson, H., Spradley, C., Li, L., Kim, I.K., Kumar, S., Hong, N., Arroliga, A.C., and Gupta, S. (2013). Circulating miRNAs as potential marker for pulmonary hypertension. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0064396"},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Xu, J., Linneman, J., Zhong, Y., Yin, H., Xia, Q., Kang, K., and Gou, D. (2022). MicroRNAs in Pulmonary Hypertension, from Pathogenesis to Diagnosis and Treatment. Biomolecules, 12.","DOI":"10.3390\/biom12040496"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/nrd.2016.246","article-title":"MicroRNA therapeutics: Towards a new era for the management of cancer and other diseases","volume":"16","author":"Rupaimoole","year":"2017","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/s11883-022-01007-9","article-title":"RNA Therapeutics: The Next Generation of Drugs for Cardiovascular Diseases","volume":"24","author":"Bejar","year":"2022","journal-title":"Curr. Atheroscler Rep."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1093\/eurheartj\/ehaa898","article-title":"Novel antisense therapy targeting microRNA-132 in patients with heart failure: Results of a first-in-human Phase 1b randomized, double-blind, placebo-controlled study","volume":"42","author":"Hauke","year":"2021","journal-title":"Eur. Heart J."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1093\/eurheartj\/ehaa791","article-title":"CDR132L improves systolic and diastolic function in a large animal model of chronic heart failure","volume":"42","author":"Batkai","year":"2021","journal-title":"Eur. Heart J."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s10238-020-00627-2","article-title":"The current state of MiRNAs as biomarkers and therapeutic tools","volume":"20","author":"Gareev","year":"2020","journal-title":"Clin. Exp. Med."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/9\/10\/533\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:41:07Z","timestamp":1760143267000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/9\/10\/533"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,28]]},"references-count":119,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["vetsci9100533"],"URL":"https:\/\/doi.org\/10.3390\/vetsci9100533","relation":{},"ISSN":["2306-7381"],"issn-type":[{"type":"electronic","value":"2306-7381"}],"subject":[],"published":{"date-parts":[[2022,9,28]]}}}