{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T08:06:34Z","timestamp":1776326794722,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,9,15]],"date-time":"2019-09-15T00:00:00Z","timestamp":1568505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["IF\/00314\/2015"],"award-info":[{"award-number":["IF\/00314\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/77912\/2011"],"award-info":[{"award-number":["SFRH\/BPD\/77912\/2011"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/AMB\/50017\/2019"],"award-info":[{"award-number":["UID\/AMB\/50017\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003176","name":"Ministerio de Educaci\u00f3n, Cultura y Deporte","doi-asserted-by":"publisher","award":["EST15\/00405"],"award-info":[{"award-number":["EST15\/00405"]}],"id":[{"id":"10.13039\/501100003176","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013276","name":"Interreg","doi-asserted-by":"publisher","award":["NOR-WATER (0725)"],"award-info":[{"award-number":["NOR-WATER (0725)"]}],"id":[{"id":"10.13039\/100013276","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003381","name":"Minist\u00e9rio da Educa\u00e7\u00e3o e Ci\u00eancia","doi-asserted-by":"publisher","award":["UID\/AMB\/50017\/2019"],"award-info":[{"award-number":["UID\/AMB\/50017\/2019"]}],"id":[{"id":"10.13039\/501100003381","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["UID\/AMB\/50017\/2019"],"award-info":[{"award-number":["UID\/AMB\/50017\/2019"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Water"],"abstract":"<jats:p>In this work, zebrafish embryo bioassays were performed to assess the efficiency of microalgae in the removal of acetaminophen from water. Chlorella sorokiniana (CS), Chlorella vulgaris (CV) and Scenedesmus obliquus (SO) were the strains used for water treatment. Toxic effects on zebrafish embryo caused by effluents from microalgae treatment were compared with those observed under exposure to experimental solutions with known concentrations of acetaminophen. The three microalgae strains allowed for the reduction of acetaminophen concentration and its toxic effects, but CS was the most efficient one. At the end of the batch culture, a 67% removal was provided by CS with a reduction of 62% in the total abnormalities on the exposed zebrafish embryo. On the other hand, toxic effects observed under exposure to effluents treated by microalgae were alike to those determined for acetaminophen experimental solutions with equivalent concentration. Thus, it may be inferred that microalgae biodegradation of acetaminophen did not involve an increased toxicity for zebrafish embryo.<\/jats:p>","DOI":"10.3390\/w11091929","type":"journal-article","created":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T03:17:57Z","timestamp":1568603877000},"page":"1929","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Acetaminophen Removal from Water by Microalgae and Effluent Toxicity Assessment by the Zebrafish Embryo Bioassay"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0378-7518","authenticated-orcid":false,"given":"Carla","family":"Escapa","sequence":"first","affiliation":[{"name":"IMARENABIO-Institute of Environment, Natural Resources and Biodiversity, Department of Applied Chemistry and Physics, Universidad de Le\u00f3n, Av. Portugal s\/n, 24071 Le\u00f3n, Spain"},{"name":"CIMAR\/CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, Endocrine Disruptors and Emerging Contaminants Group, University of Porto, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]},{"given":"Ricardo N.","family":"Coimbra","sequence":"additional","affiliation":[{"name":"Department of Environment and Planning, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3314-0184","authenticated-orcid":false,"given":"Teresa","family":"Neuparth","sequence":"additional","affiliation":[{"name":"CIMAR\/CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, Endocrine Disruptors and Emerging Contaminants Group, University of Porto, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]},{"given":"Tiago","family":"Torres","sequence":"additional","affiliation":[{"name":"CIMAR\/CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, Endocrine Disruptors and Emerging Contaminants Group, University of Porto, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]},{"given":"Miguel M.","family":"Santos","sequence":"additional","affiliation":[{"name":"CIMAR\/CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, Endocrine Disruptors and Emerging Contaminants Group, University of Porto, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"FCUP-Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5218-0726","authenticated-orcid":false,"given":"Marta","family":"Otero","sequence":"additional","affiliation":[{"name":"Department of Environment and Planning, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"},{"name":"CESAM-Centre for Environmental and Marine Studies, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1016\/j.chemosphere.2019.02.009","article-title":"Surface water pollution by pharmaceuticals and an alternative of removal by low-cost adsorbents: A review","volume":"222","author":"Quesada","year":"2019","journal-title":"Chemosphere"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.jbiotec.2004.03.033","article-title":"How to avoid pharmaceuticals in the aquatic environment","volume":"113","author":"Larsen","year":"2004","journal-title":"J. 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