{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T14:18:06Z","timestamp":1768745886407,"version":"3.49.0"},"reference-count":71,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,11]],"date-time":"2020-10-11T00:00:00Z","timestamp":1602374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation for Science and Technology (FCT)","award":["PDE\/BDE\/114582\/2016"],"award-info":[{"award-number":["PDE\/BDE\/114582\/2016"]}]},{"name":"Foundation for Science and Technology (FCT)","award":["UIDP\/50017\/2020+UIDB\/50017\/2020"],"award-info":[{"award-number":["UIDP\/50017\/2020+UIDB\/50017\/2020"]}]},{"name":"Foundation for Science and Technology (FCT)","award":["UIDB\/04295\/2020"],"award-info":[{"award-number":["UIDB\/04295\/2020"]}]},{"name":"Empresa Portuguesa das \u00c1guas Livres (EPAL)","award":["PDE\/BDE\/114582\/2016"],"award-info":[{"award-number":["PDE\/BDE\/114582\/2016"]}]},{"DOI":"10.13039\/501100005765","name":"Universidade de Lisboa","doi-asserted-by":"publisher","award":["502662875"],"award-info":[{"award-number":["502662875"]}],"id":[{"id":"10.13039\/501100005765","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Water"],"abstract":"<jats:p>Monitoring the quality of water is a requisite to prevent outbreaks related to waterborne diseases, predominantly caused by pathogens like enteric viruses, usually transmitted via the fecal-oral route. This study aimed to survey a group of enteric viruses (Enterovirus, Norovirus genogroups I and II, and hepatitis A virus) in two surface water sources of drinking water, also intending to evaluate the extent of their elimination in the two water treatment plants (WTPs) involved in drinking water production. Correlations between these viruses and fecal indicator bacteria (FIB) were also evaluated. Positive samples for viral RNA were recurrently found by reverse transcription quantitative PCR (RT-qPCR) and quantified, in genomic copies per liter (gc\/L) of sampled water. Viral RNAs were detected in 14 out of 27 samples of surface water, and 21 out of 36 samples of drinking water, NoV II having been the most frequently detected in both (0\u201378.6 gc\/L and 0\u201312.5 gc\/L, respectively). Both WTPs showed variable efficacies in the elimination of viral RNA. Only one correlation was found with FIB, between NoV II and intestinal enterococci. These results recommend the monitoring of enteric viruses over time and their inclusion in the mandatory analysis of water quality.<\/jats:p>","DOI":"10.3390\/w12102824","type":"journal-article","created":{"date-parts":[[2020,10,17]],"date-time":"2020-10-17T07:23:22Z","timestamp":1602919402000},"page":"2824","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Monitoring Waterborne Pathogens in Surface and Drinking Waters. Are Water Treatment Plants (WTPs) Simultaneously Efficient in the Elimination of Enteric Viruses and Fecal Indicator Bacteria (FIB)?"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2278-9877","authenticated-orcid":false,"given":"Daniel","family":"Salvador","sequence":"first","affiliation":[{"name":"Instituto de Sa\u00fade Ambiental, Faculdade de Medicina da Universidade de Lisboa, Avenida Prof. Egas Moniz, Edif\u00edcio Egas Moniz, Piso 0, Ala C, 1649-028 Lisboa, Portugal"},{"name":"Centro de Estudos do Ambiente e do Mar (CESAM), Departamento de Biologia Vegetal, Faculdade de Ci\u00eancias da Universidade de Lisboa, Edif\u00edcio C2-Piso 4, Campo Grande, 1749-016 Lisboa, Portugal"},{"name":"Dire\u00e7\u00e3o de Laborat\u00f3rios e Controlo da Qualidade da \u00c1gua (LAB) da Empresa Portuguesa das \u00c1guas Livres (EPAL), Avenida de Berlim, 15, 1800-031 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0559-6344","authenticated-orcid":false,"given":"Maria Filomena","family":"Caeiro","sequence":"additional","affiliation":[{"name":"Centro de Estudos do Ambiente e do Mar (CESAM), Departamento de Biologia Vegetal, Faculdade de Ci\u00eancias da Universidade de Lisboa, Edif\u00edcio C2-Piso 4, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"given":"F\u00e1tima","family":"Serejo","sequence":"additional","affiliation":[{"name":"Instituto de Sa\u00fade Ambiental, Faculdade de Medicina da Universidade de Lisboa, Avenida Prof. Egas Moniz, Edif\u00edcio Egas Moniz, Piso 0, Ala C, 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8316-5035","authenticated-orcid":false,"given":"Paulo","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Instituto de Sa\u00fade Ambiental, Faculdade de Medicina da Universidade de Lisboa, Avenida Prof. Egas Moniz, Edif\u00edcio Egas Moniz, Piso 0, Ala C, 1649-028 Lisboa, Portugal"}]},{"given":"Rui Neves","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Dire\u00e7\u00e3o de Laborat\u00f3rios e Controlo da Qualidade da \u00c1gua (LAB) da Empresa Portuguesa das \u00c1guas Livres (EPAL), Avenida de Berlim, 15, 1800-031 Lisboa, Portugal"}]},{"given":"C\u00e9lia","family":"Neto","sequence":"additional","affiliation":[{"name":"Dire\u00e7\u00e3o de Laborat\u00f3rios e Controlo da Qualidade da \u00c1gua (LAB) da Empresa Portuguesa das \u00c1guas Livres (EPAL), Avenida de Berlim, 15, 1800-031 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,11]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2020, May 23). 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