{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T13:48:22Z","timestamp":1768830502719,"version":"3.49.0"},"reference-count":63,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T00:00:00Z","timestamp":1609372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04423\/2020 and UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020 and UIDP\/04423\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013284","name":"Executive Agency for Small and Medium-sized Enterprises","doi-asserted-by":"publisher","award":["EASME\/EMFF\/2016\/1.2.1.4\/010"],"award-info":[{"award-number":["EASME\/EMFF\/2016\/1.2.1.4\/010"]}],"id":[{"id":"10.13039\/100013284","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NORTE 2020","award":["POCI-01-0145-FEDER-24055"],"award-info":[{"award-number":["POCI-01-0145-FEDER-24055"]}]},{"DOI":"10.13039\/501100002924","name":"FEDER","doi-asserted-by":"publisher","award":["PTDC\/BTA-GES\/32186\/2017 and POCI-01-0145-FEDER-032186"],"award-info":[{"award-number":["PTDC\/BTA-GES\/32186\/2017 and POCI-01-0145-FEDER-032186"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Water"],"abstract":"<jats:p>Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera Pseudomonas, Rhodococcus and Acinetobacter. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA\/P) and bioaugmentation with inoculum pre-grown in acetate (BA\/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA\/P and BA\/A but not in NA. Acetate was the best substrate for consortium growth. BA\/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills.<\/jats:p>","DOI":"10.3390\/w13010066","type":"journal-article","created":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T14:31:49Z","timestamp":1609425109000},"page":"66","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9569-804X","authenticated-orcid":false,"given":"Rafaela","family":"Perdig\u00e3o","sequence":"first","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edif\u00edcio do Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6836-0331","authenticated-orcid":false,"given":"C. Marisa R.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edif\u00edcio do Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Filipa","family":"Santos","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edif\u00edcio do Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7181-0540","authenticated-orcid":false,"given":"Maria F.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edif\u00edcio do Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Ana P.","family":"Mucha","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edif\u00edcio do Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"Faculty of Sciences, University of Porto, Rua do Campo Alegre 790, 4150-171 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103655","DOI":"10.1016\/j.marpol.2019.103655","article-title":"Oil tanker risks on the marine environment: An empirical study and policy implications","volume":"108","author":"Jin","year":"2019","journal-title":"Mar. Policy"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1098\/rsif.2009.0495","article-title":"The complex network of global cargo ship movements","volume":"7","author":"Kaluza","year":"2010","journal-title":"J. R. Soc. Interface"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1039\/C5EM00070J","article-title":"Oil spill problems and sustainable response strategies through new technologies","volume":"17","author":"Ivshina","year":"2015","journal-title":"Environ. Sci. Process. Impacts"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1007\/s00284-015-0825-7","article-title":"Marine Oil-Degrading Microorganisms and Biodegradation Process of Petroleum Hydrocarbon in Marine Environments: A Review","volume":"71","author":"Xue","year":"2015","journal-title":"Curr. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"941810","DOI":"10.4061\/2011\/941810","article-title":"Microbial degradation of petroleum hydrocarbon contaminants: An overview","volume":"2011","author":"Das","year":"2011","journal-title":"Biotechnol. Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1128\/mr.54.3.305-315.1990","article-title":"Microbial degradation of hydrocarbons in the environment","volume":"54","author":"Leahy","year":"1990","journal-title":"Microbiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Chamy, R., and Rosenkranz, F. (2013). Crude oil biodegradation in the marine environments. Biodegradation-Engineering and Technology, InTech.","DOI":"10.5772\/50829"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.marpolbul.2013.12.024","article-title":"A review of oil, dispersed oil and sediment interactions in the aquatic environment: Influence on the fate, transport and remediation of oil spills","volume":"79","author":"Gong","year":"2014","journal-title":"Mar. Poll. Pollut."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.aqpro.2015.02.058","article-title":"Weathering of Oil Spill: Modeling and Analysis","volume":"4","author":"Mishra","year":"2015","journal-title":"Aquat. Procedia"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"15200","DOI":"10.1007\/s11356-015-4458-y","article-title":"The effect of oil spills on the bacterial diversity and catabolic function in coastal sediments: A case study on the Prestige oil spill","volume":"22","author":"Wittich","year":"2015","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.marpolbul.2016.02.035","article-title":"Potential biodegradation of crude petroleum oil by newly isolated halotolerant microbial strains from polluted Red Sea area","volume":"111","author":"Shetaia","year":"2016","journal-title":"Mar. Pollut. Bull."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.scitotenv.2016.10.064","article-title":"The culturable mycobiota of a Mediterranean marine site after an oil spill: Isolation, identification and potential application in bioremediation","volume":"576","author":"Bovio","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1007\/s12010-015-1603-5","article-title":"A Comprehensive Review of Aliphatic Hydrocarbon Biodegradation by Bacteria","volume":"176","author":"Abbasian","year":"2015","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_14","first-page":"725","article-title":"Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids","volume":"6","author":"Qin","year":"2005","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.envint.2008.07.018","article-title":"Biodegradation potential of the genus Rhodococcus","volume":"35","author":"Martinkova","year":"2009","journal-title":"Environ. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1007\/s00253-005-1932-3","article-title":"The remarkable Rhodococcus erythropolis","volume":"67","year":"2005","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3219","DOI":"10.1007\/s11270-012-1103-y","article-title":"Characterisation of Oil-Degrading Bacteria Isolated from Bilge Water","volume":"223","author":"Cappello","year":"2012","journal-title":"Water Air Soil Pollut."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.copbio.2007.04.006","article-title":"Obligate oil-degrading marine bacteria","volume":"18","author":"Yakimov","year":"2007","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e01594","DOI":"10.1016\/j.heliyon.2019.e01594","article-title":"Culturable hydrocarbonoclastic marine bacterial isolates from Indonesian seawater in the Lombok Strait and Indian Ocean","volume":"5","author":"Syakti","year":"2019","journal-title":"Heliyon"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.jenvman.2012.07.034","article-title":"A sustainable approach to controlling oil spills","volume":"113","author":"Adebayo","year":"2012","journal-title":"J. Environ. Manag."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.marpolbul.2016.03.017","article-title":"Bioremediation technologies for polluted seawater sampled after an oil-spill in Taranto Gulf (Italy): A comparison of biostimulation, bioaugmentation and use of a washing agent in microcosm studies","volume":"106","author":"Crisafi","year":"2016","journal-title":"Mar. Pollut. Bull."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.marpolbul.2016.02.073","article-title":"Biodegradation of marine crude oil pollution using a salt-tolerant bacterial consortium isolated from Bohai Bay, China","volume":"105","author":"Li","year":"2016","journal-title":"Mar. Pollut. Bull."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"22099","DOI":"10.1080\/19443994.2015.1134351","article-title":"Isolation and characterization ofCandida vishwanathiistrain TERI MS1 for degradation of petroleum hydrocarbons in marine environment","volume":"57","author":"Priya","year":"2016","journal-title":"Desalination Water Treat."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1007\/s11356-012-1240-2","article-title":"Potential of the microbial community present in an unimpacted beach sediment to remediate petroleum hydrocarbons","volume":"20","author":"Almeida","year":"2013","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"463","DOI":"10.2516\/ogst:2003029","article-title":"Bioremediation of marine oil spills","volume":"58","author":"Prince","year":"2003","journal-title":"Oil Gas Sci. Technol."},{"key":"ref_26","first-page":"802","article-title":"Biostimulation strategies for fresh and chronically polluted marine environments with petroleum hydrocarbons","volume":"84","author":"Nikolopoulou","year":"2009","journal-title":"J. Chem. Technol. Biotechnol. Int. Res. Process Environ. Clean Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.scitotenv.2019.02.153","article-title":"Microbial bioremediation of oil contaminated seawater: A survey of patent deposits and the characterization of the top genera applied","volume":"666","author":"Villela","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1007\/s11274-009-0044-0","article-title":"Autochthonous bioaugmentation and its possible application to oil spills","volume":"25","author":"Hosokawa","year":"2009","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.jhazmat.2015.01.038","article-title":"Allochthonous bioaugmentation in ex situ treatment of crude oil-polluted sediments in the presence of an effective degrading indigenous microbiome","volume":"287","author":"Fodelianakis","year":"2015","journal-title":"J. Hazard. Mater"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.biortech.2016.10.073","article-title":"Biodegradation of crude oil by a defined co-culture of indigenous bacterial consortium and exogenous Bacillus subtilis","volume":"224","author":"Tao","year":"2017","journal-title":"Bioresour. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Mapelli, F., Scoma, A., Michoud, G., Aulenta, F., Boon, N., Borin, S., Kalogerakis, N., and Daffonchio, D. (2017). Biotechnologies for Marine Oil Spill Cleanup: Indissoluble Ties with Microorganisms. Trends Biotechnol.","DOI":"10.1016\/j.tibtech.2017.04.003"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/j.nbt.2013.06.005","article-title":"Microcosm evaluation of autochthonous bioaugmentation to combat marine oil spills","volume":"30","author":"Nikolopoulou","year":"2013","journal-title":"New Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.ibiod.2012.04.022","article-title":"Bacterial community dynamics during the preferential degradation of aromatic hydrocarbons by a microbial consortium","volume":"74","author":"Bacosa","year":"2012","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"ref_34","first-page":"349","article-title":"Microbial community structure of a heavy fuel oil-degrading marine consortium: Linking microbial dynamics with polycyclic aromatic hydrocarbon utilization","volume":"73","author":"Vila","year":"2010","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.marpolbul.2013.08.029","article-title":"Potential of bioremediation for buried oil removal in beaches after an oil spill","volume":"76","author":"Pontes","year":"2013","journal-title":"Mar. Pollut. Bull."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3634","DOI":"10.1007\/s11356-013-2365-7","article-title":"Bioremediation potential of microorganisms from a sandy beach affected by a major oil spill","volume":"21","author":"Reis","year":"2014","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.marpolbul.2018.04.063","article-title":"Indigenous microbial communities along the NW Portuguese Coast: Potential for hydrocarbons degradation and relation with sediment contamination","volume":"131","author":"Gouveia","year":"2018","journal-title":"Mar. Pollut. Bull."},{"key":"ref_38","unstructured":"Elisabeth Helmke, B.D., Birte Gerdes, B.D.J., Juergens, B.D.K., and Reuter, L.D. (2013). Bioremediation Method for Accelerated Biological Decomposition of Petroleum Hydrocarbons in Sea-Iced Covered Polar Regions, and Bacteria and Enzyme Mixtures as Agents for Carrying Out Said Method. (8,444,962 B2), U.S. Patent."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101877","DOI":"10.1016\/j.resourpol.2020.101877","article-title":"Environmental knowledge spillovers and productivity: A patent analysis for large international firms in the energy, water and land resources fields","volume":"69","author":"Aldieri","year":"2020","journal-title":"Resour. Policy"},{"key":"ref_40","unstructured":"Gouveia, V. (2015). Bioremediation of Oil Along the Nw Portuguese Coast-the Role of Autochthonous Microorganisms. [Master\u2019s Thesis, University of Porto]. Available online: https:\/\/repositorio-aberto.up.pt\/handle\/10216\/87315."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1139\/m96-037","article-title":"Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most-probable-number procedure","volume":"42","author":"Wrenn","year":"1996","journal-title":"Can. J. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/s00253-010-2589-0","article-title":"Microbial biosurfactants production, applications and future potential","volume":"87","author":"Banat","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0958-1669(02)00316-6","article-title":"Biosurfactants and oil bioremediation","volume":"13","author":"Ron","year":"2002","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Domingues, P.M., Oliveira, V., Serafim, L.S., Gomes, N., and Cunha, \u00c2. (2020). Biosurfactant Production in Sub-Oxic Conditions Detected in Hydrocarbon-Degrading Isolates from Marine and Estuarine Sediments. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17051746"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1111\/j.1365-2672.2006.03267.x","article-title":"An oil-degrading bacterium: Rhodococcus erythropolis strain 3C-9 and its biosurfactants","volume":"102","author":"Peng","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1007\/s11274-017-2401-8","article-title":"Characterization of biosurfactants produced by the oil-degrading bacterium Rhodococcus erythropolis S67 at low temperature","volume":"34","author":"Luong","year":"2018","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2057","DOI":"10.1007\/s11274-012-1008-3","article-title":"Characterization of novel diesel-degrading strains Acinetobacter haemolyticus MJ01 and Acinetobacter johnsonii MJ4 isolated from oil-contaminated soil","volume":"28","author":"Lee","year":"2012","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s10661-017-6137-6","article-title":"PAHs in water and surface sediments from Douro River estuary and Porto Atlantic coast (Portugal)-impacts on human health","volume":"189","author":"Rocha","year":"2017","journal-title":"Environ. Monit. Assess"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.envpol.2012.03.043","article-title":"High bacterial biodiversity increases degradation performance of hydrocarbons during bioremediation of contaminated harbor marine sediments","volume":"167","author":"Beolchini","year":"2012","journal-title":"Environ. Pollut."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"569","DOI":"10.18520\/cs\/v112\/i03\/569-576","article-title":"Biodegradation of Crude Oil Using Marine Bacillus species from Vadinar Coast, Gujarat, India","volume":"112","author":"Mulani","year":"2017","journal-title":"Curr. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.scitotenv.2016.12.141","article-title":"Biodegradation of the veterinary antibiotics enrofloxacin and ceftiofur and associated microbial community dynamics","volume":"581","author":"Alexandrino","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.nbt.2017.08.005","article-title":"Biodegradation of mono-, di- and trifluoroacetate by microbial cultures with different origins","volume":"43","author":"Alexandrino","year":"2018","journal-title":"New Biotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/j.scitotenv.2018.11.285","article-title":"Bioremediation of bezafibrate and paroxetine by microorganisms from estuarine sediment and activated sludge of an associated wastewater treatment plant","volume":"655","author":"Duarte","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1016\/j.jenvman.2018.11.022","article-title":"Biodegradation of enrofloxacin by microbial consortia obtained from rhizosediments of two estuarine plants","volume":"231","author":"Santos","year":"2019","journal-title":"J. Environ. Manag."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.jhazmat.2018.05.062","article-title":"16S metagenomic analysis reveals adaptability of a mixed-PAH-degrading consortium isolated from crude oil-contaminated seawater to changing environmental conditions","volume":"357","author":"Muangchinda","year":"2018","journal-title":"J. Hazard. Mater."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s11270-016-2999-4","article-title":"Biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments under different strategies: Natural attenuation, biostimulation, and bioaugmentation with Rhodococcus erythropolis T902. 1","volume":"227","author":"Lang","year":"2016","journal-title":"Water Air Soil Pollut."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.ecoenv.2018.07.065","article-title":"Co-biodegradation of pyrene and other PAHs by the bacterium Acinetobacter johnsonii","volume":"163","author":"Jiang","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/2191-0855-1-5","article-title":"Advances in utilization of renewable substrates for biosurfactant production","volume":"1","author":"Makkar","year":"2011","journal-title":"AMB Express"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1590\/S1517-838246120131276","article-title":"Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium","volume":"46","author":"Santisi","year":"2015","journal-title":"Braz. J. Microbiol."},{"key":"ref_60","first-page":"650","article-title":"Degradation of petroleum hydrocarbons by oil field isolated bacterial consortium","volume":"11","author":"Malik","year":"2012","journal-title":"Afr. J. Biotechnol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/j.marpolbul.2012.03.020","article-title":"Biodegradation of crude oil using an efficient microbial consortium in a simulated marine environment","volume":"64","author":"Bao","year":"2012","journal-title":"Mar. Pollut. Bull."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5927","DOI":"10.1128\/AEM.02251-13","article-title":"Bacterial Community Response to Petroleum Hydrocarbon Amendments in Freshwater, Marine, and Hypersaline Water-Containing Microcosms","volume":"79","author":"Jurelevicius","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_63","unstructured":"European Commission (COM\/2020\/329 Final) (2020, September 28). Communication from the Commission to the European Parliament, the Council, the European Economic and Social Committee and the Committee of the Regions, An updated Action Plan for a Sustainable, Resilient and Competitive Blue Economy in the European Union Atlantic Area. Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/?uri=COM%3A2020%3A329%3AFIN."}],"container-title":["Water"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4441\/13\/1\/66\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:48:17Z","timestamp":1760179697000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4441\/13\/1\/66"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,31]]},"references-count":63,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,1]]}},"alternative-id":["w13010066"],"URL":"https:\/\/doi.org\/10.3390\/w13010066","relation":{},"ISSN":["2073-4441"],"issn-type":[{"value":"2073-4441","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,31]]}}}