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However, few studies are available on how this measure affects the negotiation of ramped weirs by fish. This study aimed to assess the performance of an experimental ramped weir (3.00 m long with 10% slope; 0.30 m head-drop) to enhance the passage of a potamodromous cyprinid species, the Iberian barbel (Luciobarbus bocagei). Attention was given to testing the effects of the addition of a substrate, in this case cobbles, to the ramp (Nature) vs. a smooth bottom (Control), and discharge (Q; 55 L\u00b7s\u22121 and 110 L\u00b7s\u22121 (or specific discharge per unit width, q = 92 L\u00b7s\u22121\u00b7m\u22121 and 183 L\u00b7s\u22121\u00b7m\u22121)) on fish passage performance. Fish physiological responses to stress and fatigue, measured by glucose and lactate concentrations in blood samples, were also analysed. Results showed that the Nature design generally increased fish movements and successful upstream passages, and enhanced fish passage performance by enabling faster negotiations. Fish movements were also affected by increasing discharge, registering reductions with 110 L\u00b7s\u22121. Results of the physiological parameters indicate that both glucose and lactate concentrations were also influenced by discharge. The outcomes from this study present important information about fish passage performance across low-head ramped weirs and could provide data needed to help biologists and engineers to develop more effective structures to alleviate small instream obstacles.<\/jats:p>","DOI":"10.3390\/w13060765","type":"journal-article","created":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T20:17:40Z","timestamp":1615493860000},"page":"765","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of Low-Head Ramped Weirs for a Potamodromous Cyprinid: Effects of Substrate Addition and Discharge on Fish Passage Performance, Stress and Fatigue"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6573-9154","authenticated-orcid":false,"given":"Susana","family":"Amaral","sequence":"first","affiliation":[{"name":"Forest Research Centre, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2502-5533","authenticated-orcid":false,"given":"Paulo","family":"Branco","sequence":"additional","affiliation":[{"name":"Forest Research Centre, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3811-775X","authenticated-orcid":false,"given":"Filipe","family":"Rom\u00e3o","sequence":"additional","affiliation":[{"name":"CERIS\u2014Civil Engineering for Research and Innovation for Sustainability, T\u00e9cnico, University of Lisbon, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3900-1460","authenticated-orcid":false,"given":"Maria","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Forest Research Centre, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1423-5391","authenticated-orcid":false,"given":"Ant\u00f3nio","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"CERIS\u2014Civil Engineering for Research and Innovation for Sustainability, T\u00e9cnico, University of Lisbon, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1232-2560","authenticated-orcid":false,"given":"Jos\u00e9","family":"Santos","sequence":"additional","affiliation":[{"name":"Forest Research Centre, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1002\/aqc.2946","article-title":"Moving beyond fitting fish into equations: Progressing the fish passage debate in the Anthropocene","volume":"29","author":"Franklin","year":"2019","journal-title":"Aquatic Conserv. 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