{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T06:20:51Z","timestamp":1773037251971,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["project ReDEFine (POCI-01-0145-FEDER-029368) and the strategic program UIDB\/04423\/2020 and UIDP\/04423\/2020"],"award-info":[{"award-number":["project ReDEFine (POCI-01-0145-FEDER-029368) and the strategic program UIDB\/04423\/2020 and UIDP\/04423\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Water"],"abstract":"<jats:p>This work intended to assess the adaptability of bioassay with Raphidocelis subcapitata to be used as a complement to the water quality assessment parameters of reservoirs imposed by the European Water Framework Directive (WFD). Thus, water samples of Portuguese reservoirs (Miranda, Pocinho, Aguieira, and Alqueva) were analyzed in three sampling periods (spring and autumn 2019, and spring 2020). A physical and chemical report of waters was also performed. R. subcapitata assay proved to be sensitive, indicating the presence of a potential perturbation that was not always associated with chemical analysis performed. In general, in the spring samplings, the water samples showed more disturbances to R. subcapitata, which in some situations may be associated with the higher content of nutrients and metals. Microalgae assay can be an effective complementary tool to indicate the ecotoxicological potential since they responded quickly to all sample components of water samples, in a wide-ranging variety of water conditions (different sites in several reservoirs). High similarities between the final ecotoxicological and the ecological potentials, according to the WFD parameters, were detected. The ecotoxicological approach based on our results allowed to confirm that bioassays with R. subcapitata are suitable and sensible to detect perturbations.<\/jats:p>","DOI":"10.3390\/w13192605","type":"journal-article","created":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T22:50:48Z","timestamp":1632351048000},"page":"2605","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Microalgae Growth Inhibition-Based Reservoirs Water Quality Assessment to Identify Ecotoxicological Risks"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9093-6464","authenticated-orcid":false,"given":"Sara","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, 4169-007 Porto, Portugal"},{"name":"Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental (CIIMAR\/CIMAR), Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos S\/N, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6454-079X","authenticated-orcid":false,"given":"Ivo","family":"Pinto","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, 4169-007 Porto, Portugal"},{"name":"Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental (CIIMAR\/CIMAR), Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Nuno","family":"Formigo","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6686-9919","authenticated-orcid":false,"given":"Sara C.","family":"Antunes","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, 4169-007 Porto, Portugal"},{"name":"Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental (CIIMAR\/CIMAR), Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos S\/N, 4450-208 Matosinhos, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,22]]},"reference":[{"key":"ref_1","unstructured":"European Community Commission (ECC) (2000). 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