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Therefore, our aim was to address this knowledge gap with data on the accumulation ability, as well as the cytogenetic and biochemical effects in turbot (Scophthalmus maximus). To this end, we exposed turbot for seven days to a mixture of remediated metals (Rem treatments: Cd, Hg, and Pb), with and without the presence of nanoparticles (NP), and compared them with the maximum allowable concentrations (MAC treatment) for effluent discharges. We determined the metal accumulation in the blood and kidney and evaluated haematological changes (red blood cell count, haemoglobin, and mean cell haemoglobin (MCH)) and genotoxicity (erythrocytic nuclear abnormalities assay) in the blood. The results showed that remediation with non-living macroalgae significantly reduced the metallic blood and kidney burdens in the Rem treatments. Furthermore, no genotoxic potential occurred in the Rem and MAC treatments in parallel with the reduction in MCH levels in the Rem treatments, which would reflect hematopoietic disturbances in the MAC. Our results validate biosorption remediation as we achieved a considerable reduction in metal loads while maintaining the health status of fish, highlighting the importance of testing water remediation methods in the biota.<\/jats:p>","DOI":"10.3390\/w15081564","type":"journal-article","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T04:45:32Z","timestamp":1681706732000},"page":"1564","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Integrated Use of Bioaccumulation, Genotoxic, and Haematological Endpoints to Assess the Effect of Water Remediation Strategies on Fish Health: A Complementary Study"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5539-2406","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Mieiro","sequence":"first","affiliation":[{"name":"CESAM\u2014Centre for Environmental and Marine Studies & Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"},{"name":"Centre for Functional Ecology (CFE), TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"given":"Jo\u00e3o","family":"Coelho","sequence":"additional","affiliation":[{"name":"ECOMARE-Laboratory for Innovation and Sustainability of Marine Biological Resources, CESAM-Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, Estrada do Porto de Pesca Costeira, 3830-565 Gafanha da Nazar\u00e9, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7150-997X","authenticated-orcid":false,"given":"Ana","family":"Reis","sequence":"additional","affiliation":[{"name":"EPIUnit-Instituto de Sa\u00fade P\u00fablica, Universidade do Porto, Rua das Taipas, 135, 4050-600 Porto, Portugal"},{"name":"Department of Environmental Health, Portuguese National Institute of Health, Rua Alexandre Herculano, 321, 4000-055 Porto, Portugal"}]},{"given":"Diana","family":"Castro","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Paula","family":"Figueira","sequence":"additional","affiliation":[{"name":"Department of Chemistry & LAQV, REQUIMTE, University of Aveiro, 3810-193 Aveiro, Portugal"},{"name":"CIIMAR\u2013Interdisciplinary Centre of Marine and Environmental Research, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8358-8329","authenticated-orcid":false,"given":"Filipe","family":"Martinho","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE), TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"given":"Miguel","family":"Pardal","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE), TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"given":"Eduarda","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Chemistry & LAQV, REQUIMTE, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0552-9643","authenticated-orcid":false,"given":"M\u00e1rio","family":"Pacheco","sequence":"additional","affiliation":[{"name":"CESAM\u2014Centre for Environmental and Marine Studies & Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Cl\u00e1udia","family":"Lopes","sequence":"additional","affiliation":[{"name":"Department of Chemistry & LAQV, REQUIMTE, University of Aveiro, 3810-193 Aveiro, Portugal"},{"name":"Department of Chemistry & CICECO\u2014Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"(2023). 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