{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:46:08Z","timestamp":1760150768484,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T00:00:00Z","timestamp":1703808000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"REWATER","award":["ERA-NET Cofund WaterWorks 2015","DivRestore\/0004\/2020","UIDB\/04423\/2020","UIDP\/04423\/2020"],"award-info":[{"award-number":["ERA-NET Cofund WaterWorks 2015","DivRestore\/0004\/2020","UIDB\/04423\/2020","UIDP\/04423\/2020"]}]},{"name":"BioReset","award":["ERA-NET Cofund WaterWorks 2015","DivRestore\/0004\/2020","UIDB\/04423\/2020","UIDP\/04423\/2020"],"award-info":[{"award-number":["ERA-NET Cofund WaterWorks 2015","DivRestore\/0004\/2020","UIDB\/04423\/2020","UIDP\/04423\/2020"]}]},{"name":"BiodivRestore ERA-NET COFUND Action","award":["ERA-NET Cofund WaterWorks 2015","DivRestore\/0004\/2020","UIDB\/04423\/2020","UIDP\/04423\/2020"],"award-info":[{"award-number":["ERA-NET Cofund WaterWorks 2015","DivRestore\/0004\/2020","UIDB\/04423\/2020","UIDP\/04423\/2020"]}]},{"name":"FCT","award":["ERA-NET Cofund WaterWorks 2015","DivRestore\/0004\/2020","UIDB\/04423\/2020","UIDP\/04423\/2020"],"award-info":[{"award-number":["ERA-NET Cofund WaterWorks 2015","DivRestore\/0004\/2020","UIDB\/04423\/2020","UIDP\/04423\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Water"],"abstract":"<jats:p>Triops longicaudatus is a crustacean typically inhabiting temporary freshwater bodies in regions with a Mediterranean climate. These crustaceans are easily maintained in the laboratory and show a set of biological features that make them good candidates for diagnosing environmental quality and health. However, information about their responses to environmental contamination is scarce. This study characterised the locomotor responses of juvenile and adult\/mature T. longicaudatus to low concentrations of five model toxicants upon a very short 1.5 h exposure: tributyltin, mercury, lindane, sodium hypochlorite and formaldehyde. A video-tracking system was used to record the locomotor behaviour. The data were analysed with an artificial neural network to identify distinct behaviours, followed by Chi-square and Correspondence analysis to characterise the response to each toxicant. The results showed that T. longicaudatus is sensitive to aquatic contamination, particularly sodium hypochlorite. Six behaviour types were defined, which allowed for the characterisation and discrimination of the test toxicants. The results support the need for more investigation into this species and its behaviour types as an alternative to animal testing and the more apical and often invasive endpoints commonly recommended in standard guidelines.<\/jats:p>","DOI":"10.3390\/w16010126","type":"journal-article","created":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T03:28:41Z","timestamp":1703820521000},"page":"126","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Sensitivity of Triops longicaudatus Locomotor Behaviour to Detect Short Low-Level Exposure to Pollutants"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3360-3783","authenticated-orcid":false,"given":"Laura","family":"Guimar\u00e3es","sequence":"first","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1663-939X","authenticated-orcid":false,"given":"Ant\u00f3nio Paulo","family":"Carvalho","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]},{"given":"Pedro","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3395-273X","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Teixeira","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]},{"given":"Nuno","family":"Silva","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6733-5425","authenticated-orcid":false,"given":"Andr\u00e9","family":"Pereira","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9402-8327","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Amorim","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2584-1482","authenticated-orcid":false,"given":"Lu\u00eds","family":"Oliva-Teles","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1023\/A:1003168416080","article-title":"Reproductive isolation and genetic differentiation in North American species of Triops (Crustacea: Branchiopoda: 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