{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:24:31Z","timestamp":1790727871248,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T00:00:00Z","timestamp":1382572800000},"content-version":"vor","delay-in-days":296,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>In 2001, it was postulated that tumour\u2010derived exosomes could be a potent source of tumour\u2010associated antigens (TAA). Since then, much knowledge is gained on their role in tumorigenesis but only very recently tumour\u2010derived exosomes were used in dendritic cell (DC)\u2010based immunotherapy. For this, DCs were cultured <jats:italic>ex\u2010vivo<\/jats:italic> and loaded with exosomes derived from immunogenic tumours such as melanoma or glioma and re\u2010administrated to induce anti\u2010tumour responses in primary and metastatic tumour mouse models. In contrast, malignant mesothelioma (MM) is a non\u2010immunogenic tumour and because only a few mesothelioma\u2010specific TAA are known to date, we investigated whether mesothelioma\u2010derived exosomes could be used as antigen source in DC\u2010based immunotherapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mouse MM AB1 cells were used to generate tumour lysate and tumour\u2010derived exosomes. Tumour lysate was generated by 5 cycles of freeze\u2013thawing followed by sonication of AB1 cells. Tumour exosomes were collected from the AB1 cell culture supernatant and followed a stepwise ultracentrifugation. Protein quantification and electron microscopy were performed to determine the protein amount and to characterise their morphology. To test whether MM derived exosomes are immunogenic and able to stimulate an anti\u2010tumoral response, BALB\/c mice were injected with a lethal dose of AB1 tumour cells at day 0, followed by intraperitoneal injection of a single dose of DCs loaded with tumour exosomes, DCs loaded with tumour lysate, or phosphate buffered saline (PBS), at day 7.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mice which received tumour exosome\u2010loaded DC immunotherapy had an increased median and overall survival compared to mice which received tumour lysate\u2010loaded DC or PBS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this study, we showed that DC immunotherapy loaded with tumour exosomes derived from non\u2010immunogenic tumours improved survival of tumour bearing mice.<\/jats:p><\/jats:sec>","DOI":"10.3402\/jev.v2i0.22492","type":"journal-article","created":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T03:50:13Z","timestamp":1382586613000},"source":"Crossref","is-referenced-by-count":107,"title":["Tumour\u2010derived exosomes as antigen delivery carriers in dendritic cell\u2010based immunotherapy for malignant mesothelioma"],"prefix":"10.1002","volume":"2","author":[{"given":"Niken M.","family":"Mahaweni","sequence":"first","affiliation":[{"name":"Department of Pulmonary Medicine Erasmus MC Cancer Institute Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaretha E.H.","family":"Kaijen\u2010Lambers","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Erasmus MC Cancer Institute Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Dekkers","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Erasmus MC Cancer Institute Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim G.J.V.","family":"Aerts","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Erasmus MC Cancer Institute Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost P.J.J.","family":"Hegmans","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Erasmus MC Cancer Institute Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,10,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-119131"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/842849"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvj128"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00734.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2010.12.031"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/85438"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00384-5"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3747\/co.v16i3.367"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033330"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2015.2993"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-011-0537-1"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)09552-1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200501-057OC"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200909-1465OC"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2003-0238OC"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63739-X"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3456"},{"key":"e_1_2_7_19_1","first-page":"1","article-title":"Isolation and characterization of exosomes from cell culture supernatants and biological fluids","author":"Th\u00e9ry C","year":"2006","journal-title":"Curr Protoc Cell Biol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00204-X"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00005a004"}],"container-title":["Journal of Extracellular Vesicles"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.3402\/jev.v2i0.22492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T18:13:36Z","timestamp":1760638416000},"score":1,"resource":{"primary":{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.3402\/jev.v2i0.22492"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.3402\/jev.v2i0.22492"],"URL":"https:\/\/doi.org\/10.3402\/jev.v2i0.22492","archive":["Portico"],"relation":{},"ISSN":["2001-3078","2001-3078"],"issn-type":[{"value":"2001-3078","type":"print"},{"value":"2001-3078","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1]]},"article-number":"22492"}}