{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T21:26:28Z","timestamp":1794259588904,"version":"4.9.0"},"reference-count":53,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:00Z","timestamp":1700092800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Kidney360"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>The renal Na-K-2Cl and Na-Cl cotransporters are the major salt reabsorption pathways in the thick ascending limb of Henle loop and the distal convoluted tubule, respectively. These transporters are the target of the loop and thiazide type diuretics extensively used in the world for the treatment of edematous states and arterial hypertension. The diuretics appeared in the market many years before the salt transport systems were discovered. The evolving of the knowledge and the cloning of the genes encoding the Na-K-2Cl and Na-Cl cotransporters were possible thanks to the study of marine species. This work presents the history of how we came to know the mechanisms for the loop and thiazide type diuretics actions, the use of marine species in the cloning process of these cotransporters and therefore in the whole solute carrier cotransproters 12 (SLC12) family of electroneutral cation chloride cotransporters, and the disease associated with each member of the family.<\/jats:p>","DOI":"10.34067\/kid.0000000000000307","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:48Z","timestamp":1700092848000},"page":"133-141","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":6,"title":["From Fish Physiology to Human Disease: The Discovery of the NCC, NKCC2, and the Cation-Coupled Chloride Cotransporters"],"prefix":"10.34067","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4378-9043","authenticated-orcid":false,"given":"Gerardo","family":"Gamba","sequence":"first","affiliation":[{"name":"Molecular Physiology Unit, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico and Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Tlalpan, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,11,16]]},"reference":[{"issue":"2","key":"B1-20261109","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1152\/physrev.00011.2004","article-title":"Molecular physiology and pathophysiology of electroneutral cation-chloride cotransporters","volume":"85","author":"Gamba","year":"2005","journal-title":"Physiol Rev."},{"issue":"8","key":"B2-20261109","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.1021\/ja01565a079","article-title":"Benzothiadiazine dioxides as novel diuretics","volume":"79","author":"Novello","year":"1957","journal-title":"J Am Chem Soc."},{"issue":"5005","key":"B3-20261109","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1038\/208080b0","article-title":"Effect of \u201cFurosemid\u201d on chloride and water excretion in single nephrons of the kidney of the rat","volume":"208","author":"Malnic","year":"1965","journal-title":"Nature."},{"issue":"1","key":"B4-20261109","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/BF00582708","article-title":"Chloride reabsorption in the rabbit cortical thick ascending limb of the loop of Henle. 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