{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T20:32:06Z","timestamp":1786480326281,"version":"build-2736575974"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Kidney360"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:sec>\n                    <jats:title>Key Points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Using bulk and spatial transcriptomics in rat model, we defined early responses to hemorrhagic shock, rhabdomyolysis, and their combination.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Rhabdomyolysis was the primary driver of transcriptional changes, while hemorrhagic shock, rhabdomyolysis, and their combination produced synergistic and lethal response with distinct regional and molecular signatures.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>We identified central mechanisms linking trauma to tubular injury and AKI progression.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Trauma is a leading global cause of death, and AKI significantly worsens outcomes. Hemorrhagic shock (HS) and rhabdomyolysis (RM) are major contributors, yet their individual and combined effects on the kidney remain poorly defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using a clinically relevant rat model that closely mimics human trauma, we performed bulk and spatial transcriptomics to characterize early renal responses to HS, RM, and their combination (RM-HS). Commercial mouse spatial transcriptomics probes were successfully applied to rat kidney tissue, enabling cost-effective and region-specific gene expression profiling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>RM emerged as the dominant driver of transcriptional changes, while RM-HS triggered a synergistic, mortality-associated response. Comparative analyses revealed distinct regional and molecular signatures; HS suppressed metabolic activity, whereas RM induced widespread upregulation of inflammatory and stress-response pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      We propose a mechanistic framework linking these traumatic insults to tubular cell injury and death, with mitochondrial dysfunction, dysregulated lipid metabolism,\n                      <jats:italic toggle=\"yes\">Perilipin 2<\/jats:italic>\n                      expression, and ferroptosis as central components. This integrative model advances our understanding of trauma-induced renal injury and may enable the identification of novel biomarkers and therapeutic strategies to mitigate AKI severity in patients with trauma.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.34067\/kid.0000001045","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T17:00:36Z","timestamp":1765818036000},"page":"711-727","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["The Spatially Resolved Kidney Transcriptome Signatures in Rat Models of Trauma-Induced Acute Kidney Injury"],"prefix":"10.34067","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1880-7596","authenticated-orcid":false,"given":"Pierre","family":"Isnard","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM U1151, CNRS UMR8253, Institut Necker Enfants Malades (INEM), Paris, France"},{"name":"Laboratoire d\u2019Anatomie et Cytologie Pathologiques, APHP, H\u00f4pital Necker-Enfants Malades, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Laemmel","sequence":"additional","affiliation":[{"name":"DYNAMIC Team, UMRS999, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5477-6021","authenticated-orcid":false,"given":"Thibault","family":"Martinez","sequence":"additional","affiliation":[{"name":"DYNAMIC Team, UMRS999, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"},{"name":"Department of Anesthesiology and Critical Care, Percy Military Hospital, Clamart, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1454-2061","authenticated-orcid":false,"given":"Benjamin","family":"Bergis","sequence":"additional","affiliation":[{"name":"DYNAMIC Team, UMRS999, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"},{"name":"Service d'Anesth\u00e9sie-R\u00e9animation Chirurgicale, H\u00f4pital Bic\u00eatre, Universit\u00e9 Paris-Saclay, DMU 12, Assistance Publique, H\u00f4pitaux de Paris, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5098-4656","authenticated-orcid":false,"given":"Anatole","family":"Harrois","sequence":"additional","affiliation":[{"name":"DYNAMIC Team, UMRS999, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"},{"name":"Service d'Anesth\u00e9sie-R\u00e9animation Chirurgicale, H\u00f4pital Bic\u00eatre, Universit\u00e9 Paris-Saclay, DMU 12, Assistance Publique, H\u00f4pitaux de Paris, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8545-747X","authenticated-orcid":false,"given":"Alice","family":"Huertas","sequence":"additional","affiliation":[{"name":"DYNAMIC Team, UMRS999, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5374-7314","authenticated-orcid":false,"given":"Dian","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Washington University in St. Louis, St. Louis, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6420-8703","authenticated-orcid":false,"given":"Benjamin D.","family":"Humphreys","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Washington University in St. Louis, St. Louis, Missouri"},{"name":"Department of Developmental Biology, Washington University in St. Louis, St. Louis, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2085-8031","authenticated-orcid":false,"given":"Nicolas","family":"Libert","sequence":"additional","affiliation":[{"name":"DYNAMIC Team, UMRS999, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"},{"name":"Department of Anesthesiology and Critical Care, Percy Military Hospital, Clamart, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,12,15]]},"reference":[{"issue":"10159","key":"B1-20260430","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1016\/S0140-6736(18)32203-7","article-title":"Global, regional, and national age-sex-specific mortality for 282 causes of death in 195 countries and territories, 1980-2017: a systematic analysis for the global Burden of Disease Study 2017","volume":"392","author":"Abate","year":"2018","journal-title":"Lancet."},{"issue":"3","key":"B3-20260430","first-page":"620","article-title":"Trauma mortality in mature trauma systems: are we doing better? 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