{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T20:31:55Z","timestamp":1786480315233,"version":"build-2736575974"},"reference-count":39,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002997","name":"Nierstichting","doi-asserted-by":"crossref","award":["DKF15OP09"],"award-info":[{"award-number":["DKF15OP09"]}],"id":[{"id":"10.13039\/501100002997","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002997","name":"Nierstichting","doi-asserted-by":"crossref","award":["DKF15OP09"],"award-info":[{"award-number":["DKF15OP09"]}],"id":[{"id":"10.13039\/501100002997","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Kidney360"],"published-print":{"date-parts":[[2026,7]]},"abstract":"\n Key Points<\/jats:title>\n \n \n \n Stable kidney transplant recipients with early stage 1 lymphoid aggregates displayed gene signatures indicative of a tolerogenic immune environment.<\/jats:p>\n <\/jats:list-item>\n \n Participants with early stage 1 lymphoid aggregates demonstrated a trend toward better kidney function trajectories and lower risk of graft failure.<\/jats:p>\n <\/jats:list-item>\n \n Early stage 1 lymphoid aggregates may represent beneficial immune surveillance mechanisms promoting long-term graft stability in low-risk transplant recipients.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Background<\/jats:title>\n The presence of lymphoid tissues and their effect on long-term kidney graft outcome remains unclear. This study investigates the occurrence, molecular and phenotypic characteristics of lymphoid aggregates (LA) in protocol biopsies of clinically stable kidney transplant recipients, and their correlation with long-term clinical outcome.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We retrospectively analyzed 414 protocol biopsies from a multicenter cohort of 278 stable kidney transplant recipients taken at 6, 12, or 24 months post-transplant. On a subset of 16 biopsies at 6 months post-transplant, mRNA gene expression analysis was performed using Nanostring Banff Human Organ Transplant panel alongside phenotypic analysis with immunostaining for developmental staging of LA. Finally, the presence of LA was correlated to various clinical kidney allograft outcomes.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Stage 1 LA were characterized by upregulation of genes linked to immune regulation (\n LAG3, CTLA4, FOXP3<\/jats:italic>\n ),\n T<\/jats:italic>\n -cell signaling, and chemotaxis pathways, with enrichment of regulatory\n T<\/jats:italic>\n cells, memory\n T<\/jats:italic>\n cells,\n T<\/jats:italic>\n follicular helper cells, and dendritic cells. Immunohistochemistry confirmed higher numbers of FOXP3+ regulatory\n T<\/jats:italic>\n cells and dendritic cells in stage 1 LA, while high endothelial venules were absent. Over follow-up, participants with stage 1 LA showed slightly higher eGFR values and a trend toward better graft survival compared with those without LA or with stage 2 LA, although most differences did not reach statistical significance.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n In stable kidney transplant recipients, stage 1 LA displayed molecular and phenotypic features consistent with a regulated immune environment. The molecular and immunohistochemical analysis of stage 1 LA suggests a more regulatory molecular phenotype and composition, which may contribute to better long-term graft survival observed in this cohort of functionally stable transplant recipients. Observed trends toward more favorable outcomes suggest that early LA may not be deleterious and could represent local immune regulation, although larger prospective studies are needed to confirm these findings.<\/jats:p>\n <\/jats:sec>","DOI":"10.34067\/kid.0000001188","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T02:00:07Z","timestamp":1773972007000},"page":"1633-1647","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Early Lymphoid Aggregates in Protocol Kidney Allograft Biopsies"],"prefix":"10.34067","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9679-1182","authenticated-orcid":false,"given":"Romy","family":"du Long","sequence":"first","affiliation":[{"name":"Department of Pathology, Amsterdam UMC, Location Amsterdam Medical Center, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nike","family":"Claessen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Amsterdam UMC, Location Amsterdam Medical Center, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4454-6270","authenticated-orcid":false,"given":"Frederike J.","family":"Bemelman","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Section of Nephrology, Amsterdam UMC, Location Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2637-4046","authenticated-orcid":false,"given":"Joost C.","family":"van den Born","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, University Medical Center Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7694-8381","authenticated-orcid":false,"given":"Soufian","family":"Meziyerh","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands"},{"name":"Leiden Transplant Center, Leiden University Medical Center (LUMC), Leiden University, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1846-1193","authenticated-orcid":false,"given":"Dave L.","family":"Roelen","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands"},{"name":"Department of Immunology, HLA Laboratory, Leiden University Medical Center (LUMC), Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0904-3969","authenticated-orcid":false,"given":"Jan-Stephan F.","family":"Sanders","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, University Medical Center Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9284-3595","authenticated-orcid":false,"given":"Aiko P.J.","family":"de Vries","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands"},{"name":"Leiden Transplant Center, Leiden University Medical Center (LUMC), Leiden University, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9676-1722","authenticated-orcid":false,"given":"Sandrine","family":"Florquin","sequence":"additional","affiliation":[{"name":"Department of Pathology, Amsterdam UMC, Location Amsterdam Medical Center, University of Amsterdam, Amsterdam, The Netherlands"},{"name":"Amsterdam Institute for Infection and Immunity, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper","family":"Kers","sequence":"additional","affiliation":[{"name":"Department of Pathology, Amsterdam UMC, Location Amsterdam Medical Center, University of Amsterdam, Amsterdam, The Netherlands"},{"name":"Division of Nephrology, Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands"},{"name":"Department of Pathology, Systems Pathology Research Group, Leiden Transplant Center, Leiden University Medical Center, Leiden, The Netherlands"},{"name":"Department of Pathology, Erasmus MC Transplant Institute, Erasmus Medical Center, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,3,19]]},"reference":[{"issue":"10","key":"B1-20260731","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/s41581-019-0185-9","article-title":"Immunology of the ageing kidney","volume":"15","author":"Sato","year":"2019","journal-title":"Nat Rev Nephrol."},{"issue":"7","key":"B2-20260731","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1038\/nri3700","article-title":"Ectopic lymphoid-like structures in infection, cancer and autoimmunity","volume":"14","author":"Pitzalis","year":"2014","journal-title":"Nat Rev Immunol."},{"issue":"8","key":"B3-20260731","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/s41581-023-00706-z","article-title":"The roles of tertiary lymphoid structures in chronic 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