{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T17:00:38Z","timestamp":1784912438580,"version":"3.55.0"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Kidney360"],"abstract":"\n Background:<\/jats:title>\n \n Pressure-induced constriction (PIC) of renal arterial vasculature has been proposed to protect delicate glomerular capillaries from barotrauma. Previous studies from our laboratory have shown that degenerin proteins, including the beta subunit of the Epithelial Na\n +<\/jats:sup>\n Channel (\u03b2ENaC), are important mediators of the PIC response.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n We generated a smooth muscle \u03b2ENaC knockout mouse model (SMC-\u03b2ENaC\n -\/-<\/jats:sup>\n ) to determine the importance of smooth muscle \u03b2ENaC to the phenotype seen in \u03b2ENaC hypomorph mice. We assessed renal interlobar vascular reactivity using myography, renal inflammation and injury using histology, blotting, ELISA and flow cytometry, and blood pressure using plethysmography.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n We found that vasoconstrictor agonist (KCl, phenylephrine)-induced responses were similar in SMC-\u03b2ENaC\n -\/-<\/jats:sup>\n and control mice. However, PIC responses in the renal interlobar artery were abolished in SMC-\u03b2ENaC\n -\/-<\/jats:sup>\n mice. SBP was measured by tail-cuff plethysmography from 6 \u2013 26 weeks of age, SBP was greater in SMC-\u03b2ENaC\n -\/-<\/jats:sup>\n male (139\u00b12 vs 120.3\u00b11 mm Hg) and female (134\u00b10.8 vs 117.3\u00b13) mice compared to control littermates. We also found mild glomerular injury in SMC-\u03b2ENaC\n -\/-<\/jats:sup>\n mice at 26 weeks. By 16 weeks of age, we found elevated levels of leukocytes, including M1\/M2 macrophages, neutrophils, T-cells, and B-cells in renal arterial tissue in SMC-\u03b2ENaC\n -\/-<\/jats:sup>\n mice.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n Our findings provide additional evidence that vascular smooth muscle cell (VSMC) \u03b2ENaC contributes to the transduction of the PIC response in renal vessels. Moreover, our findings provide evidence of the protective nature of PIC against vascular renal injury.<\/jats:p>\n <\/jats:sec>","DOI":"10.34067\/kid.0000001306","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T16:01:50Z","timestamp":1784908910000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Smooth Muscle \u03b2ENaC Deletion Induces Moderate Hypertension and Renal Injury"],"prefix":"10.34067","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0023-0511","authenticated-orcid":false,"given":"Tawhida","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3973-0329","authenticated-orcid":false,"given":"Zoltan","family":"Nemeth","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth","family":"Lirette","sequence":"additional","affiliation":[{"name":"Department of Data Sciences, University of Mississippi Medical Center, Jackson, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0122-0833","authenticated-orcid":false,"given":"Erin B.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3785-853X","authenticated-orcid":false,"given":"Shujie","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Medicine: Renal-Electrolyte, University of Pittsburgh, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2413-5415","authenticated-orcid":false,"given":"Thomas R.","family":"Kleyman","sequence":"additional","affiliation":[{"name":"Department of Medicine: Renal-Electrolyte, University of Pittsburgh, USA"},{"name":"Department of Cell Biology, University of Pittsburgh, USA"},{"name":"Department of Pharmacology and Chemical Biology, University of Pittsburgh, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7171-804X","authenticated-orcid":false,"given":"Heather A.","family":"Drummond","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,7,24]]},"container-title":["Kidney360"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/10.34067\/KID.0000001306","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T16:01:50Z","timestamp":1784908910000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/10.34067\/KID.0000001306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,24]]},"references-count":0,"URL":"https:\/\/doi.org\/10.34067\/kid.0000001306","relation":{},"ISSN":["2641-7650"],"issn-type":[{"value":"2641-7650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,24]]},"assertion":[{"value":"2026-03-16","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-08","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}