{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T20:31:35Z","timestamp":1786480295174,"version":"build-2736575974"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Scientist Development","award":["1K01HL135461"],"award-info":[{"award-number":["1K01HL135461"]}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Kidney360"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Key Points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Sox6 controls the increased renin expression that is induced during renal artery stenosis, and thus has a novel function in renovascular hypertension.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Sox6 knockout in Ren1d+ cells inhibited the renovascular hypertension and kidney injury induced by renal artery stenosis.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>The results presented in this manuscript point to a new transcriptional regulatory network in renal artery stenosis which is controlled by Sox6.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Renal artery stenosis (RAStenosis) or renal artery occlusion is an intractable problem affecting about 6% of people &gt;65 and up to 40% of people with coronary or peripheral vascular disease in the Unites States. The renal renin-angiotensin-aldosterone system plays a key role in RAStenosis, with renin (which is mainly produced in the kidney) being recognized as the driver of the disease. In this study, we will determine a new function for the transcription factor Sox6 in the control of renal renin during RAStenosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We hypothesize that knocking out Sox6 in Ren1d-positive cells will protect mice against renovascular hypertension and kidney injury. To test our hypothesis, we used a new transgenic mouse model, Ren1d\n                      <jats:sup>cre<\/jats:sup>\n                      \/Sox6\n                      <jats:sup>fl\/fl<\/jats:sup>\n                      (Sox6 KO), in which Sox6 is knocked out in renin-expressing cells. We used a modified two-kidney, one-clip (2K1C) Goldblatt mouse model to induce RAStenosis and renovascular hypertension. BP was measured using the tail-cuff method. Renin, prorenin, Sox6, and NGAL expressions levels were measured with Western blot,\n                      <jats:italic toggle=\"yes\">in situ<\/jats:italic>\n                      hybridization, and immunohistochemistry. Creatinine levels were measured using the colorimetric assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Systolic BP was significantly lower in Sox6 KO 2 weeks after RAStenosis compared with Sox6 WT (Ren1d\n                      <jats:sup>cre<\/jats:sup>\n                      \/Sox6\n                      <jats:sup>wt\/wt<\/jats:sup>\n                      ). Renin, prorenin, and NGAL expression levels in the stenosed kidney were lower in Sox6 KO compared with Sox6 WT mice. Furthermore, creatinine clearance was preserved in Sox6 KO compared with Sox6 WT mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our data indicate that Sox6 controls renal renin and prorenin expression and, as such, has a function in renovascular hypertension induced by RAStenosis. These results point to a novel transcriptional regulatory network controlled by Sox6.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.34067\/kid.0002792020","type":"journal-article","created":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T17:50:34Z","timestamp":1616781034000},"page":"842-856","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":13,"title":["The Transcription Factor Sox6 Controls Renin Expression during Renal Artery Stenosis"],"prefix":"10.34067","volume":"2","author":[{"given":"Mohammad","family":"Saleem","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz","family":"Saavedra-S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierina","family":"Barturen-Larrea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8148-8308","authenticated-orcid":false,"given":"Jose A.","family":"Gomez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2021,5,27]]},"reference":[{"key":"B1-20250825","first-page":"46N","article-title":"Prevalence of atherosclerotic renal artery stenosis in patients with atherosclerosis elsewhere","volume":"88","author":"Olin","year":"1990","journal-title":"Am J Med"},{"key":"B2-20250825","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1161\/CIRCULATIONAHA.117.032582","article-title":"Potential US population impact of the 2017 ACC\/AHA high blood pressure guideline","volume":"137","author":"Muntner","year":"2018","journal-title":"Circulation"},{"key":"B3-20250825","doi-asserted-by":"crossref","first-page":"2866","DOI":"10.1161\/01.CIR.98.25.2866","article-title":"Prospective study of atherosclerotic disease progression in the renal artery","volume":"98","author":"Caps","year":"1998","journal-title":"Circulation"},{"key":"B4-20250825","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/S0002-8703(98)70138-3","article-title":"Progression of renal artery stenosis in patients undergoing cardiac catheterization","volume":"136","author":"Crowley","year":"1998","journal-title":"Am Heart J"},{"key":"B5-20250825","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1084\/jem.59.3.347","article-title":"Studies on experimental hypertension: I. 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