{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T23:13:01Z","timestamp":1788649981279,"version":"build-2803163510"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["JP18K07857"],"award-info":[{"award-number":["JP18K07857"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["JP18K07830"],"award-info":[{"award-number":["JP18K07830"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["JP18K07029"],"award-info":[{"award-number":["JP18K07029"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Kidney360"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The development of glomerulosclerosis in FSGS is associated with a reduction in podocyte number in the glomerular capillary tufts. Although it has been reported that the number of urinary podocytes in FSGS exceeds that of minimal-change nephrotic syndrome, the nature of events that promote podocyte detachment in FSGS remains elusive.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, we provide detailed, morphologic analysis of the urinary podocytes found in FSGS by examining the size of the urinary podocytes from patients with FSGS, minimal-change nephrotic syndrome, and GN. In addition, in urinary podocytes from patients with FSGS and minimal-change nephrotic syndrome, we analyzed podocyte hypertrophy and mitotic catastrophe using immunostaining of p21 and phospho-ribosomal protein S6.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The size of the urinary podocytes was strikingly larger in samples obtained from patients with FSGS compared with those with minimal-change nephrotic syndrome and GN (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.008). Urinary podocytes from patients with FSGS had a higher frequency of positive immunostaining for p21 (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt;0.001) and phospho-ribosomal protein S6 (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.02) than those from patients with minimal-change nephrotic syndrome. Characteristic features of mitotic catastrophe were more commonly observed in FSGS than in minimal-change nephrotic syndrome urinary samples (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We posit that the significant increase in the size of urinary podocytes in FSGS, compared with those in minimal-change nephrotic syndrome, may be explained by hypertrophy and mitotic catastrophe.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.34067\/kid.0005612020","type":"journal-article","created":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T18:45:12Z","timestamp":1606848312000},"page":"477-486","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":8,"title":["Morphologic Analysis of Urinary Podocytes in Focal Segmental Glomerulosclerosis"],"prefix":"10.34067","volume":"2","author":[{"given":"Yoko","family":"Shirai","sequence":"first","affiliation":[{"name":"Department of Pediatric Nephrology, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenichiro","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Central Clinical Laboratory, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Horita","sequence":"additional","affiliation":[{"name":"Department of Pathology, Kidney Center, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Pathology, Kidney Center, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Seino","sequence":"additional","affiliation":[{"name":"Department of Pathology, Kidney Center, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Ando","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsutoshi","family":"Shiratori","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoo","family":"Yabuuchi","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoto","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Ishiwa","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyonobu","family":"Ishizuka","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Hara","sequence":"additional","affiliation":[{"name":"Iwamuro Health Promotion Center, Niigata, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoshi","family":"Hattori","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Tokyo Women\u2019s Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2021,3,25]]},"reference":[{"key":"B1-20250825","doi-asserted-by":"crossref","first-page":"3162","DOI":"10.1681\/ASN.2014080752","article-title":"Glomerular aging and focal global glomerulosclerosis: A podometric perspective","volume":"26","author":"Hodgin","year":"2015","journal-title":"J Am Soc Nephrol"},{"key":"B2-20250825","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1159\/000013302","article-title":"Urinary excretion of podocytes reflects disease 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