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A degradable scaffold with regenerative capacity could overcome this limitation.\n                    <jats:bold>Methods:<\/jats:bold>\n                    A porous brushite framework was combined with decellularized periosteal matrix and decellularized bone matrix to mimic periosteal, cortical, and medullary niches. In\u00a0vitro bone marrow mesenchymal stem cell (BMSC) assays and an in\u00a0vivo rat femoral critical-sized defect model assessed osteogenesis, angiogenesis, and biomechanics.\n                    <jats:bold>Results:<\/jats:bold>\n                    The scaffold enhanced BMSC adhesion, osteogenic, and angiogenic activities in\u00a0vitro. In\u00a0vivo, it promoted complete defect bridging, vascularized bone formation, direct integration, and biomechanical restoration. Histology revealed progressive scaffold replacement via accelerated endochondral ossification.\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    This biomimetic scaffold effectively coordinates vascularization and bone regeneration, achieving functional single-stage repair of load-bearing segmental defects.\n                  <\/jats:p>","DOI":"10.34133\/bmef.0321","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:13:11Z","timestamp":1790154791000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark_01","source":"Crossref","is-referenced-by-count":0,"title":["A Biomimetic Scaffold for Single-Stage Reconstruction of Load-Bearing Segmental Bone Defects"],"prefix":"10.34133","volume":"7","author":[{"given":"Jianzhong","family":"Bai","sequence":"first","affiliation":[{"name":"Medical 3D Printing Center, Orthopedic Institute, Department of Orthopedic Surgery, The First Affiliated Hospital, School of Basic Medical 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