{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T07:25:04Z","timestamp":1787815504178,"version":"build-2784847793"},"reference-count":47,"publisher":"American Association for the Advancement of Science (AAAS)","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32100450 and 32471370"],"award-info":[{"award-number":["32100450 and 32471370"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12372302"],"award-info":[{"award-number":["12372302"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016691","name":"Guangdong Provincial Pearl River Talents Program","doi-asserted-by":"publisher","award":["2021QN02Y781"],"award-info":[{"award-number":["2021QN02Y781"]}],"id":[{"id":"10.13039\/100016691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012669","name":"Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission","doi-asserted-by":"publisher","award":["CSTB2023NSCQ-MSX0918"],"award-info":[{"award-number":["CSTB2023NSCQ-MSX0918"]}],"id":[{"id":"10.13039\/501100012669","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["spj.science.org"],"crossmark-restriction":true},"short-container-title":["Biomater Res"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n            The interplay between nuclear architecture and extracellular matrix stiffness orchestrates cell fate decisions, yet the molecular mechanisms remain poorly defined. Here, we investigate the role of Lamin A (\n            <jats:italic toggle=\"yes\">LMNA<\/jats:italic>\n            ), a nuclear structural protein whose expression correlates with tissue stiffness, in regulating cellular differentiation and fate decision. Using myoblasts and fibroblasts as models, it was observed that cells with low\n            <jats:italic toggle=\"yes\">LMNA<\/jats:italic>\n            expression showed that higher cell deformation elevated expression of neurological genes and exhibited potential for differentiation into a neural-like fate. CUT&amp;Tag sequencing of\n            <jats:italic toggle=\"yes\">LMNA<\/jats:italic>\n            -knockdown cells revealed a reduction in the size of Lamin B1-associated domains, with enhanced Lamin B1 binding at muscle-related genes (\n            <jats:italic toggle=\"yes\">Myf5<\/jats:italic>\n            and\n            <jats:italic toggle=\"yes\">Myf6<\/jats:italic>\n            ) and diminished binding at the neural gene\n            <jats:italic toggle=\"yes\">Nes<\/jats:italic>\n            , suggesting that changes in gene expression are associated with alterations in chromatin structure. Further analysis identified the dissolution of H3K9me2\/3-labeled heterochromatin regions and their redistribution in the nucleoplasm following\n            <jats:italic toggle=\"yes\">LMNA<\/jats:italic>\n            inhibition. Soft substrates (0.2 kPa) amplify the neural differentiation capacity in\n            <jats:italic toggle=\"yes\">LMNA<\/jats:italic>\n            -knockout cells. Additionally, retinoic acid was shown to enhance the expression of neurologically related genes by suppressing\n            <jats:italic toggle=\"yes\">LMNA<\/jats:italic>\n            expression. These findings reveal a novel substrate stiffness-induced mechanism by which Lamin A regulates cell fate transitions and provide a new approach for neural cell generation.\n          <\/jats:p>","DOI":"10.34133\/bmr.0256","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T16:53:49Z","timestamp":1756400029000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark_01","source":"Crossref","is-referenced-by-count":7,"title":["Decreasing Lamin A Triggers Cell Fate Transitions through Heterochromatin-Nuclear Periphery Detethering"],"prefix":"10.34133","volume":"29","author":[{"given":"Lijuan","family":"Sun","sequence":"first","affiliation":[{"name":"Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, \rChongqing University, Chongqing 400030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafan","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, \rChongqing University, Chongqing 400030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyan","family":"Zuo","sequence":"additional","affiliation":[{"name":"Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, \rChongqing University, Chongqing 400030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen 518132, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, \rChongqing University, Chongqing 400030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iqra","family":"Ali","sequence":"additional","affiliation":[{"name":"Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, \rChongqing University, Chongqing 400030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Peng","sequence":"additional","affiliation":[{"name":"Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen 518132, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juhui","family":"Qiu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, \rChongqing University, Chongqing 400030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","published-online":{"date-parts":[[2025,9,18]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.044"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00644-5"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202104682"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-022-00493-6"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00480-z"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38770-5"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-54577-4"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9051306"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2121816119"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200419670"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1240104"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2020.12.016"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e17-06-0393"},{"issue":"15","key":"e_1_3_4_15_2","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202308253","article-title":"Targeting nuclear mechanics mitigates the fibroblast invasiveness in pathological dermal scars induced by matrix stiffening","volume":"11","author":"Fu X","year":"2024","unstructured":"Fu X, Taghizadeh A, Taghizadeh M, Li CJ, Lim NK, Lee JH, Kim HS, Kim HW. 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